Isolation and molecular identification of proteolytic bacteria from vaname shrimp (Lithopenaeus Vannamei) ponds as probiotic agents

Abstract

Vannamei shrimp culture in intensive and traditional ponds is developing rapidly in Indonesia. The development starts with technology, high density, and feeding systems. It results in very high organic material produced in the aquaculture ponds, and this water quality management is necessarily maintained. This study aims to isolate and identify the molecular of proteolytic bacteria from Vannamei shrimp aquaculture ponds using a different system that is as probiotic agents. This study used a survey method and random sampling technique from traditional and intensive shrimp aquaculture. The results showed that the proportion of proteolytic bacteria in traditional ponds was higher than that in intensive ponds. The screening based on colony morphology and highest activity index obtained 4 bacterial isolates of Vibrio sp. and Pseudoalteromonas white an identity value of 98.23-99.80%. The value of this activity was observed at 7.0 cm, subsequently inhibiting Vibrio sp. by 1.5 cm. The bacteria were also found to develop at pH 7 and a temperature of 28°C adequately. Additionally, the maximum proteolytic activity at a 48-hours incubation indicated their potential relevance as a biotechnological probiotic. Further studies on the potential of Pseudoalteromonas ganghwensis strains found that the bacteria have antimicrobial activity and are sensitive to antibiotics that can be used as aquaculture probiotic agents.

Isolation and Screening of Proteolytic Bacteria from Rice-Fish Farming System Sediments

“…These materials serve as a source of protein for proteolytic bacteria [8]. According to previous research, the abundance of protein sources in the pond facilitates the dominance of proteolytic bacteria to carry out their function as protein decomposers [49]. The high protein content provides a suitable environment for their growth and development [50].…”

Isolation and Characteristics of Bioflocculant Producing Bacteria from Vannamei Shrimp Ponds

“…Vannamei shrimp cultivation with an intensive system in Indonesia has experienced a very high increase (Rangka & Gunarto, 2012 ;Amalisa et al, 2021 ;Deriyanti et al, 2021).. Shrimp cultivation with an intensive system is carried out by stocking large amounts of shrimp and providing high feed (Farras et al, 2017 ;Satyantini et al, 2020), so these waters can contain a large amount of organic matter (Arifin et al, 2018 ;Ningrum et al, 2019). Organic materials contained in intensive ponds are generally utilized by various types of degrading bacteria (Masithah et al, 2016 ;Jefri et al, 2020 ;Fitriadi et al, 2023). Degrading bacteria in intensive ponds generally plays a role in improving the environment, one of which is floc-forming bacteria.…”

“…This principle opens up great opportunities for farmers and researchers, especially in the study of water quality and natural feed in aquaculture using bacterial biotechnology. In previous studies, the Pseudoalteromonas ganghwensis strain is a bacterium as a probiotic candidate that has the ability to produce protease enzymes and can suppress the growth of Vibrio parahaemolyticus which is the main pathogen of vannamei shrimp culture (Fitriadi et al, 2023). In the future, floc-forming bacteria can be packaged as probiotic products that can be utilized on a field scale or hatchery scale cultivation which can minimize land ://jazindia.com https e online at: ilabl Ava -196 -and biocontrol pathogens.…”