Isolation and Molecular Identification of Mycoplasma Hominis in Infertile Female and Male Reproductive System

Bahaabadi, Samaneh Jamalizadeh; Moghadam, Naeime Mohseni; Kheirkhah, Babak; Farsinejad, Alireza; Habibzadeh, Victoria

doi:10.5812/numonthly.22390

Cited by 14 publications

(12 citation statements)

References 9 publications

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“…Thirty‐five studies used PCR or culture to isolate specific bacteria, namely Chlamydia trachomatis , Ureaplasma spp. and Mycoplasma spp 9,19,51,52,55,60‐89 . The PCR technique identified C trachomatis in 15 studies with reported prevalence of between 0.3% and 43.3% in infertile men, which is in contrast to the culture technique where this species was not identified in any of the studies (0/20).…”

Section: Evidence Synthesismentioning

confidence: 88%

The semen microbiome and its impact on sperm function and male fertility: A systematic review and meta‐analysis

et al. 2020

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Background Male factor is attributable in up to 50% of cases of infertility. In vitro studies demonstrate that bacteria can negatively impact sperm function. The use of next‐generation sequencing techniques has provided a better understanding of the human microbiome, and dysbiosis has been reported to impact health. Evidence regarding the impact of the semen microbiome on sperm function and fertility remains conflicting. Materials and methods A systematic search was conducted in accordance with the Preferred Reporting Items for Reviews and Meta‐analysis (PRISMA) statement. The databases MEDLINE, OVID and PubMed were searched to identify English language studies related to the identification of bacteria in the semen of infertile and fertile men, between 1992 and 2019. Fifty‐five observational studies were included, with 51 299 subjects. We included studies identifying bacteria using next‐generation sequencing, culture or polymerase chain reaction. Results The semen microbiome was rich and diverse in both fertile and infertile men. Three NGS studies reported clustering of the seminal microbiome with a predominant species. Lactobacillus and Prevotella were dominant in respective clusters. Lactobacillus was associated with improvements in semen parameters. Prevotella appeared to exert a negative effect on sperm quality. Bacteriospermia negatively impacted sperm concentration and progressive motility, and DNA fragmentation index (DFI; MD: 3.518, 95% CI: 0.907 to 6.129, P = .008). There was an increased prevalence of ureaplasma urealyticum in infertile men (OR: 2.25, 95% CI: 1.47‐3.46). Ureaplasma urealyticum negatively impacted concentration and morphology. There was no difference in the prevalence of chlamydia trachomatis between fertile and infertile men and no significant impact on semen parameters. Enterococcus faecalis negatively impacted total motility, and Mycoplasma hominis negatively impacted concentration, PM and morphology. Discussion and conclusions Ureaplasma urealyticum, Enterococcus faecalis, Mycoplasma hominis and Prevotella negatively impact semen parameters, whereas Lactobacillus appears to protect sperm quality. These findings may facilitate the development of novel therapies (eg probiotics), although the evidence regarding the impact of the seminal microbiome on fertility is inconclusive and further studies are needed to investigate this association.

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Section: Evidence Synthesismentioning

confidence: 88%

The semen microbiome and its impact on sperm function and male fertility: A systematic review and meta‐analysis

et al. 2020

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“…In our study, in patient and control groups, 10.09% and 4.58% of women had vaginal infections, respectively. Studies showed that M. genitalium is a common causative agent of STDs in men and women and is a reason for infertility among infected people [16]. In Iran, Amirmozafari et al [17] reported that in 210 genital swabs taken from 210 patients, mycoplasma strains were isolated from 39.5% of patients using a selective mycoplasma isolation media, but using PCR 57.1% of patient samples were found to be positive for mycoplasmas.…”

Section: Discussionmentioning

confidence: 99%

A Case–control Study on the Relationship between Mycoplasma genitalium Infection in Women with Normal Pregnancy and Spontaneous Abortion using Polymerase Chain Reaction

Ramazanzadeh

Khodabandehloo

Farhadifar

et al. 2016

Osong Public Health and Research Perspectives

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ObjectivesMycoplasma genitalium infections are suggested as causes of a number of pathological outcomes in pregnant women. The aim of this study was to evaluate the frequency of M. genitalium infections among pregnant women and its association with spontaneous abortion.MethodsIn this case–control study we included 109 women with spontaneous abortion with a gestational age of 10–20 weeks (patients), and 109 women with normal pregnancy with a gestational age of 20–37 weeks (controls) in Sanandaj, Iran. Using specific primers and extracted DNA from endocervical swabs, a polymerase chain reaction was conducted for the detection of M. genitalium infection in both groups.ResultsThe frequency of M. genitalium infection in patient and control groups was one (0.91%) and three (2.75%), respectively. In both control and patient groups using Fisher test, no association between mycoplasma infection and spontaneous abortion was seen.ConclusionM. genitalium may be positive in the genital tract of some pregnant women but was not associated with spontaneous abortion. Further powerful studies with larger sample sizes are needed for the determination of a possible role of M. genitalium in pregnancy outcomes and spontaneous abortion.

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“…Additionally, U. urealyticum usually causes urogenital tract infection, and it is often accompanied by M. hominis infection (Jamalizadeh et al, 2014;Lee et al, 2016;Moragianni et al, 2019). Therefore, we need to further improve the RPA-Cas12a-LFS detection system to detect M. hominis and U. urealyticum infection, such as developing a multi-detective platform combined with other Cas proteins, which is more meaningful for clinical practice.…”

Section: Discussionmentioning

confidence: 99%

Development of a RPA-CRISPR-Cas12a Assay for Rapid, Simple, and Sensitive Detection of Mycoplasma hominis

et al. 2022

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Graphical AbstractSchematic of Mycoplasma hominis nucleic acid detection based on the CRISPR-Cas12a system. Clinical samples from cervical or urethral swabs were collected and incubated with lysis buffer to release nucleic acid (10 min). Extracted DNA (1 μl) is subjected to the RPA reaction with specific primers at 37°C. After 20 min, RPA product was subjected to the CRISPR-Cas12a reaction for cleavage. The collateral nuclease activity of Cas12a (250 nM) proteins were activated upon specific binding to crRNA (crRNA3, 62.5 nM) and the DNA product; thus, Cas12a cut the quenched fluorescent ssDNA reporter (125 nM) (30 min). The generated fluorescence signal would be measured by a fluorescence plate reader or visualized by lateral flow strips.

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Isolation and Molecular Identification of Mycoplasma Hominis in Infertile Female and Male Reproductive System

Cited by 14 publications

References 9 publications

The semen microbiome and its impact on sperm function and male fertility: A systematic review and meta‐analysis

The semen microbiome and its impact on sperm function and male fertility: A systematic review and meta‐analysis

A Case–control Study on the Relationship between Mycoplasma genitalium Infection in Women with Normal Pregnancy and Spontaneous Abortion using Polymerase Chain Reaction

Development of a RPA-CRISPR-Cas12a Assay for Rapid, Simple, and Sensitive Detection of Mycoplasma hominis

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