Isolation and optimization of plumbagin production in root callus of Plumbago zeylanica L. augmented with chitosan and yeast extract

Singh, Tikkam; Sharma, Upasana; Agrawal, Veena

doi:10.1016/j.indcrop.2020.112446

Cited by 34 publications

(13 citation statements)

References 59 publications

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“…Yeast extract is one of the most common natural elicitors used in in vitro culture to induce secondary metabolites production (Cheng et al 2013;Karalija et al 2020). Yeast extract has been used successfully in various studies to improve the production of secondary metabolites such as rosmarinic acid (Gonçalves et al 2019), sanguinarine (Guízar-González et al 2016), xanthone (Krstić-Milošević et al 2017) iso avonoid (Rani et al 2020), plumbagin (Singh et al 2020) and astragalosides (Park et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

Farjaminezhad

Garoosi

2021

Preprint

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Neem is the medicinal plant used as antimalarial, antibacterial, antiviral, insecticide, and antimicrobial. This study aimed to investigate and prediction the effect of yeast extract and sampling time on cell growth, secondary metabolites synthesis, SQS1 and MOF1 genes expression by response surface methodology. The highest fresh and dry cell weights were 580.25 g/L and 21.01 g/L obtained 6 days after using 100 mg/L yeast extract. The highest azadirachtin accumulation and production were 16.08 mg/g DW and 219.78 mg/L obtained 2 and 4 days after using 25 mg/L yeast extract. Maximum mevalonic acid accumulation and production were 1.75 mg/g DW and 23.77 mg/L observed 2 days after application of 50 mg/L yeast extract. The highest amount of squalene accumulation (0.22 mg/g DW) and production (4.53 mg/L) were achieved 4 days after using 50 mg/L yeast extract. Prediction results showed that the highest azadirachtin accumulation (13.61 mg/g DW) and production (190.50 mg/L), mevalonic acid accumulation (0.50 mg/g DW) and production (5.57 mg/L), and squalene accumulation (0.30 mg/g DW) obtained by using 245 mg/L yeast extract for 2 days, 71 mg/L yeast extract for 2 days, 200 mg/L yeast extract for 4.96 days, without yeast extract for 6.54 days and 4 days, respectively. Also, predicted that the highest squalene production is achieved by long-term exposure to high concentrations of yeast extract. The qRT-PCR analysis showed the maximum relative gene expression of SQS1 and MOF1 obtained by using 150 mg/L yeast extract for 4 days and 25 mg/L yeast extract for 2 days, respectively.

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Section: Introductionmentioning

confidence: 99%

Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

Farjaminezhad

Garoosi

2021

Preprint

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“…Polyethylene glycol (PEG), NaCl and heavy metal ions are examples of such stress-inducing compounds. Adding cadmium chloride (CdCl 2 ) to the culture medium increased alliin content in in vitro cultures of garlic (Allium sativum) (Malik et al 2020) and plumbagin in the callus culture of chitrak (Plumbago zeylanica L.) (Singh et al 2020). The accumulation of secondary metabolites of shikimic acid, caffeic acid, p-coumaric acid and rosmarinic acid was increased signi cantly in the cell suspension culture of Salvia miltiorrhiza by adding 50 mmol/L of NaCl to the culture medium (Yu et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Niazian

Howyzeh

Sadat-Noori

2021

Plant Cell Tiss Organ Cult

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Bioactive compounds of medicinal plants have a wide range of applications in pharmaceutical, food and other industries. In vitro culture systems have great potential for sustainable production of bioactive compounds of medicinal plants. In the present study, the individual and combined effects of a stress tolerance-inducing (salicylic acid) and a stress-inducing elicitor (polyethylene glycol) were evaluated on regeneration e ciency, antioxidants activity and phytochemical pro le of in vitro shoot cultures of ajowan. Different concentrations of salicylic acid (SA) (0, 10, 20, 40, 80 µM) and polyethylene glycol (PEG 6000) (0, 1, 2, 5%) were added to the shoot regeneration Murashige and Skoog medium containing Kin (1.5 mg/L) and NAA (0.25 mg/L) plant growth regulators. Salicylic acid reduced the adverse effect of PEG treatment on number of regenerated shoots and in vitro rooting. The activities of catalase, superoxide dismutase, and peroxidase enzymatic antioxidants were signi cantly increased in SA + PEG treated plants. The gas chromatography-mass spectrometry (GC-MS)-pro ling revealed quantitative and qualitative phytochemical differences between control and SA + PEG treated plants. The greatest means of p-cymene and thymol bioactive compounds were obtained from in vitro shoots treated with 5% PEG + 40 µM SA. The inter-simple sequence repeats (ISSR) markers proved the genetic stability of in vitro regenerated plants. The presented protocol is useful for large-scale sustainable production of secondary metabolites (SMs) of medicinal plants. The same strategy (stress tolerance-inducing elicitor + stress-inducing elicitor) is applicable to increase valuable SMs in other production systems such as hydroponic, greenhouse and eld conditions.

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“…Tissue culture has been widely used for the large-scale regeneration of valuable medicinal plants including production of pharmaceutically active secondary metabolites through callus, cell suspension, hairy root cultures, etc. ( Singh et al 2020b). This technology has been bene cial in improving the yield of various bioactive molecules such as phenols, avonoids, alkaloids, etc.…”

Section: Introductionmentioning

confidence: 99%

Optimization of micropropagation protocol and elicitation of luteolin and rutin biomolecules using phenylalanine and chitosan in root callus of Rumex hastatus D. Don

Singh

Agrawal

2022

Plant Cell Tiss Organ Cult

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Rumex hastatus D. Don, a traditional medicinal plant known for its, antitumorous, antiangiogenic and antibacterial potential is continuously under threat of extinction due to over exploitation and climatic changes. Phytochemical analysis of different plant parts showed that roots had the maximum quantity of two bioactive molecules, luteolin and rutin and thus was selected for establishment of aseptic culture.In vitro technology has been employed for developing an e cient micropropagation protocols for its conservation, large scale production as well elicitation of the valuable bioactive compounds. Murashige and Skoog's medium supplemented with 5µM TDZ proved best for developing greenish-brown and compact calluses in 98% root cultures within 30 days. Such callus pieces on subculture to same but fresh medium differentiated an average of 10.50 ± 1.29 shoots which were elongated on MS basal medium. Such excised shoots, rooted in MS + 0.1 µM IBA medium with an average of 12.03 ± 2.49 roots per shoot.These plantlets were successfully acclimatized under greenhouse conditions and elds and their genetic delity was evaluated employing SRAP and SCoT markers which revealed their high similarity with mother plant proving their true-to-type nature. Interestingly, the in vitro regenerants showed elevated levels of phenols, avonoids, luteolin and rutin content and higher antioxidant activities over the mother plant as done by phosphomolybdenum and DPPH assays. For elicitation of bioactive compounds, 100 mg/L phenylalanine was best for enhancing optimum luteolin content (407.18 ± 10.58 µg/g d.w.) over control (22.10 ± 0.57 µg/g d.w.) almost, 17.42-fold, whereas for rutin, 25 mg/L chitosan proved most effective enhancing its synthesis up to 1169.07 ± 46.49 µg/g d.w. over control (312.41 ± 12.42 µg/g d.w.), i.e., 2.74-fold. To the best of our knowledge, this is our rst report on micropropagation and elicitation of luteolin and rutin in Rumex hastatus through root culture.

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Isolation and optimization of plumbagin production in root callus of Plumbago zeylanica L. augmented with chitosan and yeast extract

Cited by 34 publications

References 59 publications

Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Optimization of micropropagation protocol and elicitation of luteolin and rutin biomolecules using phenylalanine and chitosan in root callus of Rumex hastatus D. Don

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