Isolation and partial characterization of a protease enzyme from Thaumatococcus daniellii waste

Raimi,; Ojo, O,G,; Elemo,; B, O; Fatai,; A, A; Bankole, Paul Olusegun; H, A; Kazeem, None; M, I; Banjoko,; A, O

doi:10.5897/ajb10.2065

Cited by 11 publications

(8 citation statements)

References 12 publications

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“…The inclusion of the wastes in the optimization process (in this study) was due to the dearth of information in its use for pectinase production, with respect to the approach in this study, despite its relative abundance in parts of West Africa. [35][36][37] The acidic conditions for which accounted for the optima production of the pectinases (Figures 1 to 9) were indications of acidic pectinases. The pH conditions and relatively mild temperature conditions for optima pectinase activities indicated the suitable environment for practical application of the enzyme, as in extraction of fruit juices.…”

Section: Resultsmentioning

confidence: 99%

Comparison of Optimal Fungal Pectinase Activities Using the Box-Behnken Design

2021

TJNPR

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Pectinases are enzymes that catalyze pectin by hydrolysis and de-esterification to its simpler units. 1,2 Pectinase is reported to make up about 25 % of the global food market for enzymes, hence its importance. 3,4 The importance of this class of enzymes is mainly due to its benefits in the food industry 1 , in textile, treatment of industrial wastes, oil extraction, among other areas. 3,5,6 The significance of pectinase is in its degradation of agro wastes containing pectin, a component of the plant cell wall agricultural wastes; as pectinase and cellulase have been used singly and in combination for tomato waste treatment. 7,8 Various microorganisms such as bacteria and fungi have been reported to produce pectinase. 9,10,11 Also, many substrates have been employed for pectinase production, with variations in the activities of the enzyme produced. 10,12,13 Pectinolytic fungi in controlled environments are easy to grow; hence, their application in large-scale production of the enzyme. 14 Pectinase optimizations have been undertaken in many studies by using one factor at a time (OFAT) and the Response Surface Methodology (RSM) methods. 10,15 Pectinase optimization processes are undertaken to improve the activity of the enzyme 1,10,16

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Section: Resultsmentioning

confidence: 99%

Comparison of Optimal Fungal Pectinase Activities Using the Box-Behnken Design

2021

TJNPR

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“…2-Acetoxy trimethyl-6,9-epidioxydecalin has also been reported to shave antidiabetic potentials in insulin-responsive cells and may hence proof useful for the treatment of type 2 diabetes. Bicyclo [3.1.1] heptan-3-ol, 2,6,6 (1α,2β,3α,5α)-found in Thaumatococcus danielli leaf has been reported to exhibit antioxidant properties [12]. [1] also described flavonoids as potent antioxidants and free radical scavengers capable of protecting cell membranes from damage.…”

Section: Thaumatococus Daniellimentioning

confidence: 99%

Nutritional Composition and GC-MS Phytochemical Analysis of Thaumatococcus daniellii Leaves

Iwueke¹,

Ejekwumadu

Chukwu

et al. 2020

EJNFS

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Aims: There is a high risk associated with using artificial materials like polyethylene or foil for wrapping foods before cooking. The present study investigated the phytochemical and nutrient composition of Thaumatococus daniellii leaf, a local food wrapper. Place and Duration of Study: Biochemistry Unit, Department of Science Laboratory Technology, Imo State Polytechnic Umuagwo, between April and May 2018. Methodology: The phytochemical compounds were analyzed using gas chromatography coupled with mass spectrometry (GC-MS) while proximate and vitamin composition was determined using standard analytical methods. Results: The GC-MS phytochemical analysis revealed the presence of eight (8) bioactive compounds 1,2,3,4-Butanetetrol, d-Glycero-d-idoheptose, Cyclopentane,1-ethyl-1-methyl-, 6-Octyl-1-ol,3,7-dimethyl-, 2-Octenal, E- Octane,1-chloro-,2-Acetoxy-1,1,10-trimethyl-6,9-epidioxydecalin and Bicyclo[3.1.1]heptan-3-ol,2,6,6-trimethyl-,(1α,2β,3α,5α)- while vitamin assay results showed that Thaumatococcus daniellii leaf contained 3.1mg/100g of vitamin A, 1.07 mg/100 g vitamin B1, 1.32 mg/100 g vitamin B3, 1.11 mg/100 g of vitamin B5, 16.34 mg/100 g of vitamin B6, 11.86 mg/100 g of vitamin B12 and 25.19 mg/100 g of vitamin C. Result of proximate analysis indicates that Thaumatococcus daniellii leaf contains 10.15% ash, 9.67% moisture, 20.41% protein, 11.42% lipids, 13.78% fibre and 34.57% carbohydrate. Conclusion: In summary, our findings indicate that Thaumatococcus daniellii leaves contain bioactive compounds, an appreciable number of vitamins and high nutritional constituents. Hence Thaumatococcus daniellii leaves can be incorporated into animal feed, taking into cognizance the high protein, fat and vitamin contents. These suggest that it may play critical medicinal and nutritional roles and probably be a safer alternative to polyethylene paper and aluminum foil wraps; hence justifying its local usage as food wrapper. The leaves may also serve as a lead for the production of a healthier material for wrapping local foods.

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“…Enzymes are the catalysts that regulate many chemical reactions occurring in the living body and are called biocatalysts (Raimi et al 2011). Proteases are enzymes that promote the cleavage of peptide bonds through hydrolysis (Franco et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Production, purification, and characterization of alkaline protease from Aspergillus flavus and its compatibility with commercial detergents

Wajeeha

Asad

Mahmood

et al. 2020

BioRes

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Aspergillus flavus was used to produce alkaline protease. Solid state fermentation (SSF) strategy was adopted to explore the most favorable physical and nutritional conditions for enzyme production. Maximum production was achieved at pH 6.0 and a temperature of 30 °C after 84 h of growth period. For the optimization of the chemical parameters, different carbon and nitrogen sources were used including glucose, fructose, sucrose, ammonium sulphate, and urea. Maximum production was observed with 0.3% concentration of all the compounds. Ammonium sulphate salting out and gel chromatography was used to purify the enzyme. The enzyme was completely precipitated out at 80% saturation. The value of Vmax was 3.9 U/mL, while the value of Km was 1.9 mg/mL. The enzyme was tested for its compatibility with a few famous detergents available on the market. With the alkaline protease under study, the enzyme displayed a maximum retention of its activity i.e. 80.8% in the presence of commercial detergent Surf excel. The activity dropped down to 61.5% when the enzyme was allowed to work in the presence of another locally used detergent, i.e., Bonus. Protease production from A. flavus was carried out on rice bran and wheat bran and the wheat bran gave better results.

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Isolation and partial characterization of a protease enzyme from Thaumatococcus daniellii waste

Cited by 11 publications

References 12 publications

Comparison of Optimal Fungal Pectinase Activities Using the Box-Behnken Design

Comparison of Optimal Fungal Pectinase Activities Using the Box-Behnken Design

Nutritional Composition and GC-MS Phytochemical Analysis of Thaumatococcus daniellii Leaves

Production, purification, and characterization of alkaline protease from Aspergillus flavus and its compatibility with commercial detergents

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