Isolation and partial characterization of a mercury-binding nonhistone protein component from rat kidney nuclei

Histaminergic Pathway in Rat Brain Evidenced by Lesions of the Medial Forebrain Bundle

“…Chanda and Cherian have observed a 35to 45-fold incorporation (relative to other fractions) of labeled mercury into a nuclear protein (nonhistone) fraction (6). In their experiment tusing kidney nuclei obtained 5 to 6 hours after a single injection of mercury, no significant amount of metal accumulated in the other fractions.…”

“…These data suggest that both in the test tube and at the cellular level homopolynucleotide complexes, such as poly(A) poly(U), must be equally susceptible to fundamental structural transitions. That poly(I) may cause such alterations at the cellular level is no surprise, since poly(I) has been shown previously to form an active complex with poly(C) after the polymers had been added to cells separately in an interval of one to several hours (6). Although poly(U) reduced the activity of poly(A) poly(U) when mixed with, or applied to, the cells after poly(A) * poly(U), it failed to do so when applied to the cells before poly(A) * poly(U).…”

Heavy metal toxicities

“…A non-histone protein component into which Hg is rapidly incorporated has also been isolated from rat kidney nuclei but its synthesis is not induced by Hg, since it occurs also in non-treated rats (Chanda & Cherian, 1973). As non-histone nuclear proteins are involved in genetic transcription it has been suggested that binding of Hg to this fraction may be responsible for Hg-induced chromosomal abnormalities and genetic defects.…”

Binding and Uptake of Mercuric Chloride in Human Lymphoid Cells

“…Choie and Richter [5], in studies on liver nuclei, have shown that injection of heavy metals in vivo stimulates DNA syn thesis and they also showed the presence of a heavy metal-bound nuclear non-histone protein fraction. With kidney nuclei, Chanda and Cherian [4] found mercuric chloride to be incorporated in a non-histone protein component which was firmly bound to DNA. Synthesis of non-histone proteins and control of non histone protein phosphorylation are considered to be important in the regulation of cell proliferation [for refs see 10].…”