Isolation and pathogenicity of Serratia marcescens from adult house flies infected with Entomophthora muscae

Benoit, Thomas G.; Wilson, Gregory D.; Pryor, Nan W.; Bull, D. L.

doi:10.1016/0022-2011(90)90047-a

Cited by 9 publications

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“…Heated and unheated supernatant from cells grown in Table 4. Enzyme activity of cell extracts and supernatants of three species of bacteria reared on egg yolk (EY), fly egg (FE), and nutrient broth (NB) media yeast and peptone solution, cell-free spent broth and heat-killed cells had no effect on tsetse, house ßy, or blow ßy adults (Poinar et al 1979, Benoit et al 1990, OÕCallaghan et al 1996. Our results indicated that cells alone and supernatant alone did not cause mortality, but live cells mixed with supernatant from cells grown in egg yolk medium did cause mortality.…”

Section: Discussionmentioning

confidence: 97%

“…Serratia has also been shown to cause mortality in nonbloodfeeding Diptera. House ßies showed 50% mortality 72 h after feeding on sugar treated with Serratia applied to sugar cubes (Benoit et al 1990), and Lucilia serricata had 21Ð 45% mortality 7 d after being fed Serratia (OÕCallaghan et al 1996).…”

Section: Discussionmentioning

confidence: 98%

“…Several species of Diptera are infected by S. marcescens, including stable ßies (Watson and Petersen 1991), house ßies, Musca domestica (L.) (Benoit et al 1990) blow ßies, Lucilia sericata (Meigen), (OÕCallaghan et al 1996), and various species of tsetse (Poinar et al 1979, Onoviran et al 1985, Kaaya and Daarji 1989. Aeromonas punctata is a pathogen of the mosquito, Anopheles annulipes Walker (Kalucy and Daniel 1972).…”

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Comparsion of Selected Growth Media for CulturingSerratia marcescens, Aeromonassp., andPseudomonas aeruginosaas Pathogens of AdultStomoxys calcitrans(Diptera: Muscidae)

2002

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Stable flies, Stomoxys calcitrans (L.), were orally infected with Aeromonas sp., Pseudomonas aeruginosa (Schroeter), and Serratia marcescens Bizio that were cultured on egg-yolk media, nutrient broth, and fly egg media. Aeromonas and Serratia caused mortality when the bacteria were originally grown on egg-yolk medium. Pseudomonas was equally lethal regardless of the media on which it was cultured. A wild isolate of Aeromonas caused greater death than an isolate that had been passed through host flies and had been reisolated from killed flies. Mortality increased with bacterial dose for all species. Aeromonas and Serratia caused mortality within several days after ingestion, whereas Pseudomonas caused a gradual increase in mortality 3-7 d after ingestion. The pathologic activity of Aeromonas and Serratia required extracellular products produced when cells were grown in egg yolk medium. Aeromonas required both supernatant and cells from egg yolk medium, wereas Serratia required supernatant from egg yolk medium and cells from either nutrient broth or egg yolk medium. Mortality due to ingestion of Aeromonas was correlated with the presence of enzymes that cause alpha- and beta-hemolysis, while mortality following ingestion of Serratia was associated with alpha-hemolysins, elastases, and chitinases.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 98%

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Comparsion of Selected Growth Media for CulturingSerratia marcescens, Aeromonassp., andPseudomonas aeruginosaas Pathogens of AdultStomoxys calcitrans(Diptera: Muscidae)

2002

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“…On the other hand, S. marcescens is reported to be a nosocomial pathogen (Carrero et al, 1995) and a facultative anaerobe that multiplies quickly in the gut of many insect species, causing septicaemia and death. It is often isolated from diseased and dead insects (Benoit et al, 1990;Rodríguez, 1995;Escobar et al, 2001;Prabakaran et al, 2002;Green et al, 2005). Other species, such as S. entomophila, induce pathologies in pest insects when the bacterium is ingested (Hurst and Jackson, 2002).…”

Section: Discussionmentioning

confidence: 99%

In vitro pathogenicity of Northern Peru native bacteria on Phyllocnistis citrella Stainton (Gracillariidae: Phyllocnistinae), on predator insects (Hippodamia convergens and Chrysoperla externa), on Citrus aurantiifolia Swingle and white rats

Meca

Sepúlveda

Ogoña

et al. 2009

Span. j. agric. res.

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In Peru, the leaf miner Phyllocnistis citrella attacks citrus crops, including the economically important species Citrus aurantifolia, adversely affecting production. The objective of this work was to determine the in vitro pathogenic ability of enterobacteria isolated from within P. citrella. In addition, the pathogenic effects of these enterobacterias were tested on the predator insects Hippodamia convergens and Chrisoperna externa, on the host plant C. aurantifolia and on rats. The insects were captured in plantations of C. aurantifolia in the Piura Region. Phyllocnistys citrella was the most frequently occurring pest (98%), among other identified pests. From diseased larvae of P. citrella, the bacteria Serratia sp., Pseudomonas sp., and Enterobacter aerogenes were isolated. The three bacterial species had a similar pathogenic effect on P. citrella after 48 h (74.1% average mortality). Serratia sp. caused the highest mortality after 24 h in H. convergens (40%) and C. externa (30%), whereas the Lowest mortality rates were induced at 72 h by E. aerogenes on C. externa (3%) and by Pseudomonas sp. on H. convergens (10%). The bacteria did not affect neither C. aurantifolia or the rats, which gained the same weight as control animals.Additional key words: Enterobacter, entomopathogenic bacteria, Piura, plant health and crop protection, Pseudomonas, Serratia. ResumenPatogenicidad in vitro de bacterias nativas del norte del Perú sobre Phyllocnistis citrella Stainton (Gracillariidae: Phyllocnistinae), sobre insectos predadores (Hippodamia convergens y Chrisoperna externa), Citrus aurantifolia y ratas blancas En Perú el minador de los cítricos Phyllocnistis citrella ataca a cultivos de Citrus aurantifolia, afectando negativamente a su producción. El objetivo de este trabajo fue determinar la capacidad patogénica in vitro de enterobacterias, aisladas de P. citrella, sobre esta plaga, comparándola con el efecto de estas bacterias sobre los insectos predadores Hippodamia convergens y Chrisoperna externa, sobre la planta hospedera C. aurantifolia y sobre ratas blancas. Los insectos fueron capturados en plantaciones de la Región Piura. Phyllocnistys citrella fue la especie mas frecuente (98%) entre otras plagas identificadas. A partir de larvas enfermas de P. citrella se aislaron las bacterias Serratia sp., Pseudomonas sp. y Enterobacter aerogenes. Se determinó su actividad patogénica contra P. citrella, los insectos controladores Chrysoperla externa e Hippodamia convergens, sobre el hospedero C. aurantifolia y ratas blancas. Las tres bacterias tuvieron un efecto bacteriano similar (74.1% mortalidad promedio), desde las 48 h de inoculación, contra P. citrella. Serratia sp. indujo la mortalidad mas alta, desde las 24 h, sobre H. convergens (40%) y C. externa (30%). La mortalidad más baja fue inducida a las 72 h por E. aerogenes sobre Ch. externa (3%) y por Pseudomonas sp. sobre H. convergens (10%). Las bacterias no afectaron a C. aurantifolia ni a las ratas, las cuales aumentaron de peso igual que el control.Palabras...

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“…marcescens has been reported as a pathogen of several economically important insect pests such as the boll weevil, Anthonomus grandis Boheman (Ourth & Smalley, 1980), the tobacco hornworm, Manduca sexta (Linnaeus) (Dunn & Drake, 1983), and the house fly, Musca domestica Linnaeus (Benoit et al, 1990), as well as beneficial arthropods such as the predatory mite Metaseiulus occidentalis (Nesbitt) , and the honey bee, Apis mellifera Linnaeus (El Sanousi et al, 1987). S. marcescens also has been reported as an insect pathogen of moderate virulence that causes a fatal septicemia after penetration through the insect's gut wall and subsequent invasion ofthe hemocoel (Lysenko, 1985;Krieg, 1987).…”

Section: Discussionmentioning

confidence: 99%

Serratia marcescens as a bacterial pathogen of Rhagoletis pomonella flies (Diptera: Tephritidae)

Lauzon¹,

Bussert²,

Sjogren³

et al. 2003

Eur. J. Entomol.

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Abstract. A nonpigmenting strain of Serratia marcescens Bizio isolated from dead and apparently diseased wild apple maggot flies, Rhagoletis pomonella (Walsh), was shown to he pathogenic to healthy apple maggot flies upon ingestion. The microorganism was detected in live adult alimentary canal organs four days post ingestion hut produced death in some flies within 24 h when flies fed on a cell concentration of 4.7 x 104 cfu/ml and within 8 h when flies fed on filter-sterilized culture medium that previously contained a 21 h culture of S. marcescens. Increasing the cell concentration 10,000 fold did not lead to an increased rate of kill. Young flies (7-10 days old) were more susceptihle to infection leading to death than were older flies (21-28 days old). The potential use of S. marcescens cells as control agents against apple maggot flies is negated hy their pathogenicity to vertehrates; however, the potential use of toxic compounds produced hy this strain of S. marcescens is discussed.

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Isolation and pathogenicity of Serratia marcescens from adult house flies infected with Entomophthora muscae

Cited by 9 publications

References 0 publications

Comparsion of Selected Growth Media for CulturingSerratia marcescens, Aeromonassp., andPseudomonas aeruginosaas Pathogens of AdultStomoxys calcitrans(Diptera: Muscidae)

Comparsion of Selected Growth Media for CulturingSerratia marcescens, Aeromonassp., andPseudomonas aeruginosaas Pathogens of AdultStomoxys calcitrans(Diptera: Muscidae)

In vitro pathogenicity of Northern Peru native bacteria on Phyllocnistis citrella Stainton (Gracillariidae: Phyllocnistinae), on predator insects (Hippodamia convergens and Chrysoperla externa), on Citrus aurantiifolia Swingle and white rats

Serratia marcescens as a bacterial pathogen of Rhagoletis pomonella flies (Diptera: Tephritidae)

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