Isolation and Phylogenetic Analysis of Batai Virus, Germany

Jöst, Hanna; Bialonski, Alexandra; Schmetz, Christel; Günther, Stephan; Becker, Norbert; Schmidt‐Chanasit, Jonas

doi:10.4269/ajtmh.2011.10-0483

Cited by 62 publications

(63 citation statements)

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“…Our findings suggest a geographical/temporal association with genetic diversity between strains of Ngari virus. Similar observations have been made for Batai virus in which correlation between geographical and genetic diversity has been suggested [10,14]. Batai virus isolates from Europe, Asia and Africa generally cluster independently within strongly supported groups with country-specific viruses clustering closest [14].…”

Section: Resultssupporting

confidence: 81%

Phylogenetic analysis of Bunyamwera and Ngari viruses (family Bunyaviridae, genus Orthobunyavirus) isolated in Kenya

et al. 2015

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SUMMARYOrthobunyaviruses, tri-segmented, negative-sense RNA viruses, have long been associated with mild to severe human disease in Africa, but not haemorrhagic fever. However, during a Rift Valley fever outbreak in East Africa in 1997–1998, Ngari virus was isolated from two patients and antibody detected in several others with haemorrhagic fever. The isolates were used to identify Ngari virus as a natural Orthobunyavirus reassortant. Despite their potential to reassort and cause severe human disease, characterization of orthobunyaviruses is hampered by paucity of genetic sequences. Our objective was to obtain complete gene sequences of two Bunyamwera virus and three Ngari virus isolates from recent surveys in Kenya and to determine their phylogenetic positioning within the Bunyamwera serogroup. Newly sequenced Kenyan Bunyamwera virus isolates clustered closest to a Bunyamwera virus isolate from the same locality and a Central African Republic isolate indicating that similar strains may be circulating regionally. Recent Kenyan Ngari isolates were closest to the Ngari isolates associated with the 1997–1998 haemorrhagic fever outbreak. We observed a temporal/geographical relationship among Ngari isolates in all three gene segments suggesting a geographical/temporal association with genetic diversity. These sequences in addition to earlier sequences can be used for future analyses of this neglected but potentially deadly group of viruses.

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Section: Resultssupporting

confidence: 81%

Phylogenetic analysis of Bunyamwera and Ngari viruses (family Bunyaviridae, genus Orthobunyavirus) isolated in Kenya

et al. 2015

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“…Realtime RT-PCR was used as described. 12,13 A total of 70,378 female mosquitoes (16,057 in 2009 and 54,321 in 2010) were captured, identified to species, and pooled according to species (25 mosquitoes per pool).…”

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confidence: 99%

“…In conclusion, after detection of several arboviruses that are new to Germany in 2009 12,13 another important arbovirus was demonstrated to circulate in Germany in 2010. Thus, mosquitobased surveillance for arboviruses in Germany is important for public health because it provides data about arbovirus activity and distribution.…”

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confidence: 99%

“…12,13 Extracted RNA was analyzed by using a newly designed USUV-specific real-time reverese transcription-polymerase chain reaction (RT-PCR) with primers USUTU F (5′-CGTTCTCGACTTTGACTA-3′, nucleotide positions 3294-3311; nucleotide positions are given according to numbering in USUV strain Vienna, GenBank accession no. AY453411), USUTU R (5′-GCTAGTAGTAG TTCTTATGGA-3′, nucleotide positions 3384-3364), probe USUTU P (5′-FAM-ACCGTCACAATCACTGAAGCAT-BHQ1-3′, nucleotide positions 3325-3346, FAM, 6-carboxyfluorescein; BHQ-1, black hole quencher 1).…”

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confidence: 99%

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Isolation of Usutu Virus in Germany

Jöst¹,

Bialonski²,

Maus³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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149

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Usutu virus (USUV) is a mosquito-borne flavivirus that emerged 2001 in Austria and caused deaths in wild birds. In Germany, 70,378 female mosquitoes were captured in 2009 and 2010 and assayed for USUV. Virus was isolated in cell culture from one pool of Culex pipiens pipiens mosquitoes trapped exclusively in August 2010 in Weinheim, Germany. Subsequent phylogenetic analysis demonstrated a close relationship between the isolated USUV strain from Germany and a USUV strain from Austria, which was detected in a dead blackbird in 2004.

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“…albopictus [4][5][6]. This, together with the demonstration of several pathogenic viruses in fieldcollected mosquitoes in Germany [7][8][9], prompted the German authorities to initiate nationwide mosquito monitoring activities in 2011.…”

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confidence: 99%

Two invasive mosquito species, Aedes albopictus and Aedes japonicus japonicus, trapped in south-west Germany, July to August 2011

et al. 2012

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Adult females of two invasive species, Aedes albopictus and Aedes japonicus japonicus, were collected for the first time in July and August 2011 in Germany. Previously, only immature stages of these species had been found in the country. Repeated detection of these species reveals the Upper Rhine Valley in south-west Germany to be a particularly sensitive region for the introduction and establishment of exotic mosquito species that needs careful observation.

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Isolation and Phylogenetic Analysis of Batai Virus, Germany

Cited by 62 publications

References 10 publications

Phylogenetic analysis of Bunyamwera and Ngari viruses (family Bunyaviridae, genus Orthobunyavirus) isolated in Kenya

Phylogenetic analysis of Bunyamwera and Ngari viruses (family Bunyaviridae, genus Orthobunyavirus) isolated in Kenya

Isolation of Usutu Virus in Germany

Two invasive mosquito species, Aedes albopictus and Aedes japonicus japonicus, trapped in south-west Germany, July to August 2011

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