2020
DOI: 10.1016/j.mcp.2020.101650
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Isolation and phylogenetic analysis of Getah virus from a commercial modified live vaccine against porcine reproductive and respiratory syndrome virus

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Cited by 10 publications
(8 citation statements)
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“…1I and 1J), and housed in a cage with their GETV-free littermates. Total RNA was extracted from tissue, including spleen, lung, cerebral cortex, and various lymph nodes using TRIzol reagent after 5 days post inoculation (DPI) (Kumanomido et al, 1988), and subjected to reverse transcriptionpolymerase chain reaction (RT-PCR) for GETV detection using specific primers (GETV-F: 5′-ACCGAAGAAGCCGAAGAA-3′, and GETV-R: 5′-GCACTCRAGGTCATACTTG-3′) (Zhou et al, 2020) (Supplemental Fig. 1G and 1H).…”
Section: Infectiousness Of Mature Getv V1 Strain In Micementioning
confidence: 99%
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“…1I and 1J), and housed in a cage with their GETV-free littermates. Total RNA was extracted from tissue, including spleen, lung, cerebral cortex, and various lymph nodes using TRIzol reagent after 5 days post inoculation (DPI) (Kumanomido et al, 1988), and subjected to reverse transcriptionpolymerase chain reaction (RT-PCR) for GETV detection using specific primers (GETV-F: 5′-ACCGAAGAAGCCGAAGAA-3′, and GETV-R: 5′-GCACTCRAGGTCATACTTG-3′) (Zhou et al, 2020) (Supplemental Fig. 1G and 1H).…”
Section: Infectiousness Of Mature Getv V1 Strain In Micementioning
confidence: 99%
“…In previous studies, we have isolated a new GETV strain, V1 strain, from pregnant sows had abortions, and have demonstrated the V1 strain of GETV had a strong cytopathic effect and higher proliferation titer of vaccine (Zhou et al, 2020). In this study, we utilized mice model to investigate the infectiousness and pathogenicity of mature GETV.…”
Section: Introductionmentioning
confidence: 99%
“…The occurrence of abortion is related to female animals and closely related to the semen quality of male animals ( 13 , 14 ). The reproductive effects of GETV on male animals have not been reported ( 15 , 16 ). Therefore, we explored the effects of the Getah virus on testis, epididymis, and sperm in mice to reveal the reproductive effects of GETV in male mice.…”
Section: Introductionmentioning
confidence: 99%
“…In swine, GETV infection often result in the abortion of piglets(Xing et al 2020). Recently, GETV was detected in a commercial live-attenuated vaccine preparation aimed at preventing infections with Porcine reproductive and respiratory syndrome virus (PRRSV)(Zhou et al 2020). The contamination occurred during the manufacturing and might result in PPRSV vaccination-related GETV infections(Zhou et al 2020).…”
mentioning
confidence: 99%
“…Recently, GETV was detected in a commercial live-attenuated vaccine preparation aimed at preventing infections with Porcine reproductive and respiratory syndrome virus (PRRSV)(Zhou et al 2020). The contamination occurred during the manufacturing and might result in PPRSV vaccination-related GETV infections(Zhou et al 2020). Pigs immunized with these commercial live PPRSV-vaccines might be a source for further GETV spread to other vertebrate species that likely serve as reservoir host for GETV.Thus, there is a great need for a safe and effective GETV vaccine.…”
mentioning
confidence: 99%