Isolation and phylogenetic classification of culturable psychrophilic prokaryotes from the Collins glacier in the Antarctica

Abstract

Culturable psychrophilic prokaryotes were obtained of samples of glacier sediment, seaside mud, glacier melted ice, and Deschampsia antarctica rhizosphere from Collins glacier, Antarctica. The taxonomic classification was done by a culture-dependent molecular approach involving the Amplified Ribosomal DNA Restriction Analysis. Two hundred sixty colonies were successfully isolated and sub-cultivated under laboratory conditions. The analysis showed a bacterial profile dominated by Beta-proteobacteria (35.2%) followed by Gamma-proteobacteria (18.5%), Alpha-proteobacteria (16.6%), Gram-positive with high GC content (13%), Cytophaga-Flavobacterium-Bacteroides (13%) and Gram-positive with low GC content (3.7%). Eleven of the isolates have been reported previously and the others microorganisms remain uncharacterized. The isolated microorganisms here could be a potential source for biotechnological products, such as cold-active enzymes and secondary metabolites.

Surprising abundance of Gallionella-related iron oxidizers in creek sediments at pH 4.4 or at high heavy metal concentrations

“…19(Eriksson et al, 2005; Allgaier and Grossart, 2006; Matsuzawa et al, 2010; Nishio et al, 2010; Garcia-Echauri et al, 2011; Reiche et al, 2011);…”

Isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold Arctic fjord sediments (Svalbard, Norway)

“…Bacteria from the genus Herminiimonas have been previously reported to be psychrophilic; isolates were obtained from an Antarctic glacier (Garcia‐Echauri et al ., ), a deep (3042 m) Greenland glacial ice core (Loveland‐Curtze et al ., ), and Greenland sea ice brine (Møller et al ., ). At least two other isolates of Herminiimonas have been shown to reduce nitrate, and the Herminiimonas arsenicoxydans genome contains the nirK gene (Muller et al ., ; Lang et al ., ).…”

Biohydrogen production using psychrophilic bacteria isolated from Antarctica

“…Classification of microorganisms was done according to the identity percentage of 16S rRNA gene sequence using the criteria of Drancourt as described elsewhere [16,17]. From our collection of 54 psychrophilic strains [16], only those that were cultivable at 25 C were selected for biohydrogen production screening (Table 1).…”

“…Classification of microorganisms was done according to the identity percentage of 16S rRNA gene sequence using the criteria of Drancourt as described elsewhere [16,17]. From our collection of 54 psychrophilic strains [16], only those that were cultivable at 25 C were selected for biohydrogen production screening (Table 1). Table 1 e Taxonomic classification and biohydrogen production parameters for the psychrophilic bacteria cultured with 20 g/l of glucose, pH 6.5 and 25 C. i n t e r n a t i o n a l j o u r n a l o f h y d r o g e n e n e r g y x x x ( 2 0 1 4 ) 1 e7…”