Isolation and phylogenetic identification of a dark‐septate fungus associated with the alpine plantRanunculus adoneus

Abstract

Summary• Dark-septate endophytic (DSE) fungi are ubiquitous in the roots of Arctic and alpine plants, yet very little is known about their phylogenetic identities or effects on their host plants.• Several such fungi were isolated from the alpine snowbed plant Ranunculus adoneus in the Front Range of Colorado, USA; one isolate was chosen for detailed study. The ability of this isolate to re-colonize plant roots in pot cultures was assessed, and phylogenetic analyses were performed using small-subunit (SSU), 5.8S and internal transcribed spacer (ITS) 2 ribosomal DNA sequences.• This isolate had the ability to produce root endophytic structures in pot cultures similar to those reported from other sources and observed in R. adoneus roots. SSU phylogenetic analyses showed this isolate to be related to a clade within the Euascomycetes containing the Leotiales and Erysiphales. In addition, SSU and 5.8S -ITS2 sequences showed high phylogenetic similarity to a variety of isolates reported from other plants of diverse geographical origins. Although most of these isolates remain unidentified, one closely related isolate was the anamorphic taxon Phialophora gregata .• The results suggest that this DSE isolate might belong to the fairly closely related group of plant endophytes that have varied effects on the plants that they inhabit.

Identity, Diversity, and Molecular Phylogeny of the Endophytic Mycobiota in the Roots of Rare Wild Rice ( Oryza granulate ) from a Nature Reserve in Yunnan, China

“…While the abundance of DSEs in arctic, alpine, and temperate habitats has been investigated widely (31,39,51,52), the role of DSEs in tropical ecosystems is still poorly understood. The melanized hyphae, typical for these fungi, are considered to be of importance for the host to survive stress conditions because cell wall melanin can trap and eliminate oxygen radicals generated during abiotic stress (48).…”

Diversidad, ecología e importancia potencial de los hongos endófitos septados obscuros en México

“…& McNew incrementan las concentraciones de fósforo y nitrógeno en hojas (Saito et al, 2006;Green et al, 2008;Upson et al, 2009a). Sin embargo, los efectos positivos, neutrales o negativos hacia la planta, al parecer dependen del genotipo vegetal y fún-gico que se asocien y de la fertilidad del suelo (Schadt et al, 2001;Kernaghan y Patrinquin, 2011;Mandyam et al, 2012;Mayerhofer et al, 2013). Por otra parte, las interacciones de los DSE con otros microorganismos, como los hongos micorrízicos, han sido poco estudiadas.…”

“…No obstante que se han comprobado algunos efectos benéfi cos en planta por los DSE, en la literatura aún no se reporta el uso de inoculantes a base de éstos, además de que su propagación se ha realizado a escala mínima (Schadt et al, 2001;Zhang et al, 2008). Algunas cepas de DSE (Lepto- Zhang et al, 2012).…”

Cendawan Endofit Nonpatogen Asal Tanaman Cabai dan Potensinya sebagai Agens Pemacu Pertumbuhan

“…, pertumbuhan > kontrol; , pertumbuhan = kontrol; , pertumbuhan < kontrol. Jumlah Isolat (%) 18 16 14 12 10 8 6 4 2 0 Garut Bogor cendawan endofit, terutama kelompok endofit bersepta gelap (Schadt et al 2001). Paul et al (2012) juga melaporkan bahwa cendawan endofit mampu mengolonisasi tanaman cabai baik saat fase pembibitan, pembungaan, dan pembuahan dengan frekuensi isolasi, berturutturut 12, 21, dan 67%.…”