Isolation and preliminary characterisation of twenty-five temperature-sensitive mutants of mouse cytomegalovirus

Akel, Hazem; Sweet, C.

doi:10.1111/j.1574-6968.1993.tb06523.x

Cited by 15 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, cytolysis-resistant and drug-resistant mutants were also introduced to study the function of herpesvirus genes [25]. These technologies also contributed to the generation and characterization of mutants from other herpesviruses, such as pseudorabies virus (PRV) [26], VZV [27], and cytomegalovirus [28,29]. The use of ts mutants in MDV research has been limited because of the highly cell associated nature of the virus.…”

Section: Temperature Sensitive (Ts) Mutantmentioning

confidence: 99%

Methods for the Manipulation of Herpesvirus Genome and the Application to Marek’s Disease Virus Research

et al. 2021

View full text Add to dashboard Cite

Herpesviruses are a group of double-strand DNA viruses that infect a wide range of hosts, including humans and animals. In the past decades, numerous methods have been developed to manipulate herpesviruses genomes, from the introduction of random mutations to specific genome editing. The development of genome manipulation methods has largely advanced the study of viral genes function, contributing not only to the understanding of herpesvirus biology and pathogenesis, but also the generation of novel vaccines and therapies to control and treat diseases. In this review, we summarize the major methods of herpesvirus genome manipulation with emphasis in their application to Marek’s disease virus research.

show abstract

Section: Temperature Sensitive (Ts) Mutantmentioning

confidence: 99%

Methods for the Manipulation of Herpesvirus Genome and the Application to Marek’s Disease Virus Research

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Building on this approach, a similar "positive selection" marker strategy was adapted for the generation of cytomegalovirus mutants, using the guanosyl phosphoribosyltransferase gene (Mocarski and . Studies on temperature-sensitive mutants by random screening aimed to answer one of the most fundamental questions to address when studying a viral gene is whether the gene is essential to the viral life cycle in tissue culture as well as tropism for a specific cell type (Sweet et al, 2007;Benyesh-Melnick et al, 1975;Akel and Sweet, 1993).…”

Section: Elucidating Herpesvirus Biology With the Help Of Studying Hsmentioning

confidence: 99%

Recombinant herpesviruses as tools for the study of herpesvirus biology

Kúdelová¹,

Belvončíková²,

Halásová³

et al. 2013

View full text Add to dashboard Cite

Summary. -This article is a brief summary of efforts to generate mutant herpesviruses for investigating and assigning gene functions of herpesviruses in replication and pathogenesis. While a full review of all herpesviruses is beyond the scope of this review, we focused our attention on the prototype of the herpesvirus subfamily -herpes simplex virus and murine gammaherpesvirus that serves as an excellent animal model to study human gammaherpesvirus pathogenesis. Furthermore, our present knowledge of essential, non-essential, and common genes of herpesviruses as well as of accessory genes that are currently being studied with the help of the bacterial artificial chromosome (BAC) system will also be discussed. This system facilitates the analysis of herpesviral genes with potential for use in gene therapy or as anti-cancer therapeutics.Keywords: herpes simplex virus; murine gammaherpesvirus; bacterial artificial chromosome system; recombinant herpesvirus; herpesviral gene function Email: virukude@savba.sk; phone: +4212-59302434. Abbreviations: BAC = bacterial artificial chromosome; EBV = Epstein-Barr virus; FGARAT = N-formylglycinamide ribotide amidotransferase; HCMV = human cytomegalovirus; HHV-1-8 = human herpesviruses 1-8; HHV-6,7 = human herpesviruses 6 and 7; HSV-1,2 = herpes simplex virus 1 and 2; IRF-7 = IFN regulatory factor 7; KSHV = Kaposi΄s sarcoma-associated herpesvirus; MHV-68 = murine gammaherpesvirus 68; VZV = varicella-zoster virus

show abstract

Investigation of murine cytomegalovirus latency and reactivation in mice using viral mutants and the polymerase chain reaction

1996

Self Cite

View full text Add to dashboard Cite

Studies with 6 ts mutants of mouse cytomegalovirus indicated that mutants tsm1, tsm2, tsm3, and tsm6, like wild-type (wt) virus, produced acute infection in mice, became latent, and were reactivated as infectious virus immunosuppression. Using PCR, all five viruses expressed immediate-early (IE)-1, early (E)-1, and late (L, gB) genes during acute infection in all tissues examined (salivary glands, lung, spleen, liver, kidney, and heart). DNA was present in most tissues during latent infection with all five viruses, but transcription was restricted to the IE-1 gene in the salivary glands of wt infected mice only, suggesting true molecular latency rather than low level virus persistence. Similarly, mutant tsm5 expressed all three genes following primary inoculation. Although no detectable virus was produced, tsm5 subsequently entered the latent state as evidenced by DNA detection without RNA transcription indicating that productive infection is not required to initiate latency. This mutant also failed to reactivate from latency, although all three marker genes were expressed in most tissues. In contrast, tsm4 expressed all three marker genes and produced infectious virus during acute infection, then became latent. However, upon immunosuppression to reactivate tsm4, IE-1 and E-1 transcription occurred but neither gB transcription nor infectious virus was detectable in salivary glands, lung, spleen, liver, kidney, heart, or blood. The significance of this with regard to reactivation from latency is discussed.

show abstract

Isolation and preliminary characterisation of twenty-five temperature-sensitive mutants of mouse cytomegalovirus

Cited by 15 publications

References 14 publications

Methods for the Manipulation of Herpesvirus Genome and the Application to Marek’s Disease Virus Research

Methods for the Manipulation of Herpesvirus Genome and the Application to Marek’s Disease Virus Research

Recombinant herpesviruses as tools for the study of herpesvirus biology

Investigation of murine cytomegalovirus latency and reactivation in mice using viral mutants and the polymerase chain reaction

Contact Info

Product

Resources

About