Isolation and preliminary characterization of temperature-sensitive mutants of vaccinia virus

Condit, Richard C.; Motyczka, Arlene

doi:10.1016/0042-6822(81)90150-1

Cited by 184 publications

(184 citation statements)

References 40 publications

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“…A similar behaviour of several early promoters has been observed in vaccinia virus infections when the early-tolate switch is inhibited by AraC. 36,[42][43][44] The extended period of transgene expression observed in the primary human DC cultures suggests that antigenic stimulation provided by these cells may be long lasting. It will be important to define further the antigen-presenting and immunostimulatory capacities of rFWPV infected primary human DCs.…”

Section: Figure 7 Human DC Infected With Fowlpox Virus Retain Viabilimentioning

confidence: 59%

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Dendritic cells infected with recombinant fowlpox virus vectors are potent and long-acting stimulators of transgene-specific class I restricted T lymphocyte activity

et al. 2000

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The identification of dendritic cells (DC) as the major antigen-presenting cell type of the immune system, combined with the development of procedures for their ex vivo culture, has opened possibilities for tumour immunotherapy based on the transfer of recombinant tumour antigens to DC. It is anticipated that the most effective type of response would be the stimulation of specific, MHC class I restricted cytotoxic T lymphocytes capable of recognising and destroying tumour cells. In order to make this approach possible, methods must be developed for the transfer of recombinant antigen to the DC in such a way that they will initiate an MHC class I restricted response. Here, we demonstrate that murine DC infected with a recombinant fowlpox virus (rFWPV) vector stimulate a powerful, MHC class I restricted response against a recombinant antigen. A rFWPV containing the OVA gene was constructed and used to infect the DC line

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Section: Figure 7 Human DC Infected With Fowlpox Virus Retain Viabilimentioning

confidence: 59%

“…36 The cells were infected at a multiplicity of 10 p.f.u. per cell with rFWPV expressing LacZ under the control of an early (FpEFL2) or late (FpEFL29) promoter.…”

Section: Infection Of Dc24 Dendritic Cells Using Rfwpvmentioning

confidence: 99%

Dendritic cells infected with recombinant fowlpox virus vectors are potent and long-acting stimulators of transgene-specific class I restricted T lymphocyte activity

et al. 2000

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“…* The phenotype of each mutant was described when they were originally characterized (Condit & Motyczka, 1981: Ensinger, 1982Condit et al, 1983). N, normal DNA and protein synthesis at 40 °C; DNA-, low incorporation of [3H]thymidine at 40 °C; DL, apparently normal early protein synthesis and DNA replication, but reduced late protein synthesis at 40 °C.…”

Section: Resultsmentioning

confidence: 99%

“…The mutant viruses employed in this study have been characterized previously (Condit & Motyczka, 1981;Ensinger, 1982;Condit et al, 1983). The ability of a ts mutant virus to replicate at 40 °C (leakiness) has been determined for most of the mutants used, and ranges from 0.1% to 5%.…”

Section: Cells and Virusesmentioning

confidence: 99%

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Intragenic and intergenic recombination between temperature-sensitive mutants of vaccinia virus

Fathi

Dyster

Seto

et al. 1991

Journal of General Virology

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The frequency of recombination for a complete set of two-factor crosses between vaccinia virus mutations separated by distances of between 54 and 10 692 bp was determined. The results show that in intragenic crosses there is a linear relationship between the recombination frequency observed and distances between the mutations of up to 700 bp. However, no length dependence of the recombination frequency in intergenic crosses with a distance between mutations of 328 to greater than 10000 bp is observed. We attribute this lack of dependence to the high rate of viral DNA interchange, which makes some step other than the cross-over event rate-limiting. Furthermore, we believe that the observed difference in recombination frequency between inter-and intragenic recombination is due to complementation between temperature-sensitive mutants at the permissive temperature.

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Inhibition of CD1d1‐mediated antigen presentation by the vaccinia virus B1R and H5R molecules

et al. 2006

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Vaccinia virus (VV) has been most commonly used as the vaccine to protect individuals against the causative agent of smallpox (variola virus), but it also uses a number of strategies meant to evade or blunt the host's antiviral immune response. Natural killer T (NKT) cells are a subset of immunoregulatory CD1d‐restricted T lymphocytes believed to bridge the innate and adaptive immune responses. It is shown here that the VV‐encoded molecules, B1R and H5R, play a role in the ability of VV to inhibit CD1d‐mediated antigen presentation to NKT cells. These are the first poxvirus‐encoded molecules identified that can play such a role in the evasion of an important component of the innate immune response.

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Isolation and preliminary characterization of temperature-sensitive mutants of vaccinia virus

Cited by 184 publications

References 40 publications

Dendritic cells infected with recombinant fowlpox virus vectors are potent and long-acting stimulators of transgene-specific class I restricted T lymphocyte activity

Dendritic cells infected with recombinant fowlpox virus vectors are potent and long-acting stimulators of transgene-specific class I restricted T lymphocyte activity

Intragenic and intergenic recombination between temperature-sensitive mutants of vaccinia virus

Inhibition of CD1d1‐mediated antigen presentation by the vaccinia virus B1R and H5R molecules

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