Isolation and Preliminary Charactrization of Strptomyces Flavopersicus Mutants Blocked in Spectinomycin Biosynthesis

Abstract

Stable mutants that are blocked in the production of the amino glycoside antibiotic spectinomycin were generated by treatment of Streptomyces flavopersicus NRRL 2820 with a combination of N-methyl-N-nitro-N-nitrosoguanidine (NTG) and ultraviolet light. On the basis of their co-synthetic properties in agar-strip and mixed culture methods, the mutants were grouped into five phenotypic groups, and arranged in the most probable linear sequence of metabolic blocks.