2007
DOI: 10.1016/j.biochi.2007.03.015
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Isolation and primary structure of a cellulase from the Japanese sea urchin Strongylocentrotus nudus

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Cited by 79 publications
(66 citation statements)
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“…SnEG54 cDNAs without a stop codon were amplified from pCR2-SnEG54 cDNA [17] by PCR with KOD (Toyobo, Tokyo, Japan) using the following primers: 5'-GCCATGTTGCCTTTAATCTTG-3' (SnEG54-sense) and 5'-CATGTTGAGTTGCTTGAGGCC-3' (SnEG54-antisense). The amplified fragments were subcloned into pBluescript II SK+ (Stratagene, La Jolla, CA) at the EcoRV site (pBS-SnEG54).…”
Section: Methodsmentioning
confidence: 99%
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“…SnEG54 cDNAs without a stop codon were amplified from pCR2-SnEG54 cDNA [17] by PCR with KOD (Toyobo, Tokyo, Japan) using the following primers: 5'-GCCATGTTGCCTTTAATCTTG-3' (SnEG54-sense) and 5'-CATGTTGAGTTGCTTGAGGCC-3' (SnEG54-antisense). The amplified fragments were subcloned into pBluescript II SK+ (Stratagene, La Jolla, CA) at the EcoRV site (pBS-SnEG54).…”
Section: Methodsmentioning
confidence: 99%
“…Next, we examined cellulase activity of SnEG54 prepared from the cytosol and the culture medium as reported previously [17]. pcDNA3-MycHis-SnEG54 (20 μg) or Mock was transfected into HEK293T cells (3 x 10 6 cells/10 ml culture medium).…”
Section: Cellulase Activity Of Sneg54 Expressed In Mammalian Cellsmentioning
confidence: 99%
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“…Besides, a-amylase and cellulase were purified and characterized from the digestive organ of Strongylocentrotus nudus (Nakatani and Kobayashi, 1996;Nishida et al, 2007). More recently, we purified subtilase from the digestive organ of the short-spined sea urchin (Strongylocentrotus intermedius) by a combination of ion-exchange chromatography and gel filtration.…”
mentioning
confidence: 99%