2015
DOI: 10.1007/s10561-015-9541-6
|View full text |Cite
|
Sign up to set email alerts
|

Isolation and proliferation of umbilical cord tissue derived mesenchymal stem cells for clinical applications

Abstract: Umbilical cord (UC) is a rich source of rapidly proliferating mesenchymal stem cells (MSCs) that are easily cultured on a large-scale. Clinical applications of UC-MSCs include graft-versus-host disease, and diabetes mellitus types 1 and 2. UC-MSCs should be isolated and proliferated according to good manufacturing practice (GMP) with animal component-free medium, quality assurance, and quality control for their use in clinical applications. This study developed a GMP standard protocol for UC-MSC isolation and … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

2
50
0
2

Year Published

2016
2016
2022
2022

Publication Types

Select...
7
3

Relationship

0
10

Authors

Journals

citations
Cited by 77 publications
(54 citation statements)
references
References 50 publications
2
50
0
2
Order By: Relevance
“…WJ-MSCs compared to ALDHcounterpart. Our results were in accordance with previous studies reporting that these three genes where expressed by WJ-MSCs (Beeravolu et al 2017;Van Pham et al 2016).…”
Section: Discussionsupporting
confidence: 94%
“…WJ-MSCs compared to ALDHcounterpart. Our results were in accordance with previous studies reporting that these three genes where expressed by WJ-MSCs (Beeravolu et al 2017;Van Pham et al 2016).…”
Section: Discussionsupporting
confidence: 94%
“…mUC-MSCs from monkeys were used for this experiment to imitate hUC-MSCs used for human. Van et al [17]. analysized pluripotent cell markers showed UC-MSCs maintained the expression of the oncogenes Nanog and Oct4 after long term culture but failed to transfer tumors in NOD/SCID mice.…”
Section: Discussionmentioning
confidence: 99%
“…In addition, MSC treatment has been the focus of more than 560 clinical trials that supply exogenous or activate endogenous MSCs [10] and has become the most effective source of cell-based therapy [11,12]. Human umbilical cord MSCs (uMSCs) are a kind of MSC derived from umbilical cord through a noninvasive procedure, allowing robust in vitro culture expansion, and its physiological properties could be basically reserved under cryostorage [13][14][15]. They require relatively low cost to harvest and are promising candidates for regenerative medicine in the future.…”
Section: Introductionmentioning
confidence: 99%