Isolation and propagation of classical swine fever virus in porcine Wharton’s Jelly mesenchymal stem cells

Tomar, Neelam; Bhat, Irfan Ahmad; Bharti, Mukesh Kumar; John, Jeny K.; Sharma, Veena; Chandra, Vikash; Sharma, G. Taru; Saikumar, G.

doi:10.1080/10495398.2020.1813151

Cited by 2 publications

(1 citation statement)

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“…Mesenchymal stem cells (MSCs) are multipotent and differentiate into various mesodermal lineages such as adipose, bone, cartilage, and connective tissues. Therefore, MSCs reside in a variety of mesodermal tissues and can be derived from the adipose tissue, umbilical cord, bone marrow, and other mesodermal tissues [ 1 , 2 , 3 ]. MSCs exhibit a consistent spindle-shaped morphology and share common surface marker profiles, regardless of their origin, as defined by the International Society for Cell Therapy (ISCT) [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Porcine Adipose- and Wharton’s Jelly-Derived Mesenchymal Stem Cells

Kim,

Choi,

Park

et al. 2023

Animals

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Mesenchymal stem cells (MSCs) are promising candidates for tissue regeneration, cell therapy, and cultured meat research owing to their ability to differentiate into various lineages including adipocytes, chondrocytes, and osteocytes. As MSCs display different characteristics depending on the tissue of origin, the appropriate cells need to be selected according to the purpose of the research. However, little is known of the unique properties of MSCs in pigs. In this study, we compared two types of porcine mesenchymal stem cells (MSCs) isolated from the dorsal subcutaneous adipose tissue (adipose-derived stem cells (ADSCs)) and Wharton’s jelly of the umbilical cord (Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs)) of 1-day-old piglets. The ADSCs displayed a higher proliferation rate and more efficient differentiation potential into adipogenic and chondrogenic lineages than that of WJ-MSCs; conversely, WJ-MSCs showed superior differentiation capacity towards osteogenic lineages. In early passages, ADSCs displayed higher proliferation rates and mitochondrial energy metabolism (measured based on the oxygen consumption rate) compared with that of WJ-MSCs, although these distinctions diminished in late passages. This study broadens our understanding of porcine MSCs and provides insights into their potential applications in animal clinics and cultured meat science.

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Section: Introductionmentioning

confidence: 99%