Isolation and propagation of neural stem cells in caprine (Capra hircus)

Abstract

Neural stem cells (NSCs) can self-renew and give rise to neurons, astrocytes and oligodendrocytes; they are found in the nervous system of mammalian organisms, representing a promising resource for both fundamental research and therapeutics. There have been few investigations on NSCs in the livestock species. Therefore, we have successfully isolated and characterised NSCs from the foetal brain of a small domestic animal, the goat (called GNSCs). These cells from the foetal brain showed self-renewal, rapid proliferation with a population doubling time of 88 h, were morphologically homogeneous and maintained normal chromosome throughout the culture period. The cells expressed NSC-specific markers (Sox2, Pax6 and Mushashi), but were negative for CD34 and CD45. They were capable of multi-differentiation into neurons, astrocytes, oligodendrocytes, as well as adipocytes and osteocytes. The availability of such cells may hold great interest for basic and applied neuroscience.

Isolation and characterization of neural stem cells from ovine foetal cerebral cortex

“…The PDT was significantly prolonged, cell transparency and viability decreased beginning at passage10 (Fig 3). The 'S' shaped curve obtained shows similarity with the proliferation rate observed by Li et al (2017); Kumar et al (2013) and Agarwal et al(2014).…”

“…The cells were developing into multiple neurites extending from the cell body whose projections intertwined into a network after a week (Fig1B) as illustrated by Li et al (2017). Morphologically similar cells isolated from goat foetal brain were maintained for several passages by Agarwal et al (2014). Single long axon-like processes developed and granular structures reminiscent of Nissil substance were also observed (Fig.…”

“…The NSCs isolated from human and mouse CNS have been shown to exhibit all these markers (Osterberg and Roussa 2009). Apart from these markers, NSCs have been reported to express other NSC markers Sox2, Pax6 (Agarwal et al, 2014), GFAP (Kumar et al, 2013), Notch1, MAP (Li et al, 2017). The neural stem cells isolated from embryonic day 98 foetal goat brain transfected with green fluorescent protein gene expressed neuron specific markers (Zheng et al, 2014).…”

“…(2012) in porcine. Agarwal et al (2014) successfully isolated and characterized NSCs from the foetal brain of goat and observed that the isolated NSCs expressed neuron specific markers. Kumar et al (2013) reported that the neural stem cells can be isolated and expanded rapidly from buffalo foetal brain in adherent culture and expressed neuronal markers like β tubulin III and GFAP.…”