Isolation and Properties of a Chromosome-dependent<i>KHR</i>Killer Toxin in<i>Saccharomyces cerevisiae</i>

Goto, Kuniyasu; Iwase, Tosinori; Kichise, Kouji; Kitano, Kazuyosi; Totuka, Akira; Obata, Takaji; Hara, Shodo

doi:10.1080/00021369.1990.10869979

Cited by 9 publications

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“…Orthologs of 102 out of 218 of the non-degenerate set of these ‘non-S288c’ ORFs have been identified previously in S. cerevisiae strains, mainly through whole-genome sequencing of AWRI1631, EC1118 and RM11-1a and YJM789 [8], [9], [13] (Table S2). These include genes encoding proteins such as the Khr1 killer toxin [24] which is found in YJM789, EC1118, Vin13, VL3, FostersB and FostersO and orthologs of the MPR1 stress-resistance gene (which was originally identified in the Sigma 1278b strain[23]) in RM11-1a, EC1118, AWRI1631, JAY291, QA23 and VL3.…”

Section: Resultsmentioning

confidence: 99%

Whole-Genome Comparison Reveals Novel Genetic Elements That Characterize the Genome of Industrial Strains of Saccharomyces cerevisiae

et al. 2011

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Human intervention has subjected the yeast Saccharomyces cerevisiae to multiple rounds of independent domestication and thousands of generations of artificial selection. As a result, this species comprises a genetically diverse collection of natural isolates as well as domesticated strains that are used in specific industrial applications. However the scope of genetic diversity that was captured during the domesticated evolution of the industrial representatives of this important organism remains to be determined. To begin to address this, we have produced whole-genome assemblies of six commercial strains of S. cerevisiae (four wine and two brewing strains). These represent the first genome assemblies produced from S. cerevisiae strains in their industrially-used forms and the first high-quality assemblies for S. cerevisiae strains used in brewing. By comparing these sequences to six existing high-coverage S. cerevisiae genome assemblies, clear signatures were found that defined each industrial class of yeast. This genetic variation was comprised of both single nucleotide polymorphisms and large-scale insertions and deletions, with the latter often being associated with ORF heterogeneity between strains. This included the discovery of more than twenty probable genes that had not been identified previously in the S. cerevisiae genome. Comparison of this large number of S. cerevisiae strains also enabled the characterization of a cluster of five ORFs that have integrated into the genomes of the wine and bioethanol strains on multiple occasions and at diverse genomic locations via what appears to involve the resolution of a circular DNA intermediate. This work suggests that, despite the scrutiny that has been directed at the yeast genome, there remains a significant reservoir of ORFs and novel modes of genetic transmission that may have significant phenotypic impact in this important model and industrial species.

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Section: Resultsmentioning

confidence: 99%

Whole-Genome Comparison Reveals Novel Genetic Elements That Characterize the Genome of Industrial Strains of Saccharomyces cerevisiae

et al. 2011

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“…12 ) MID for C. glabrate, S. cerevisiae KL88, and Kluyveromyces drosophilarum NCYC 575, which were killed by strain No. 115 or the single-copy strain, ranged from 1 to 4 units, but the MID for S. cerevisiae DBY 746, NCYC 761, and Debaryomyces vanriji NCYC 577, which were only killed by the multi-copy transformant, ranged from 5 to 10 units.…”

Section: Expression Of the Khs Gene In Yeastmentioning

confidence: 99%

Cloning and Nucleotide Sequence of theKHSKiller Gene ofSaccharomyces cerevisiae

Goto

Fukuda

Kichise

et al. 1991

Agricultural and Biological Chemistry

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Kinetic studies of killer toxin K1 binding to yeast cells indicate two receptor populations

Kurzweilová

Sigler

1994

Arch Microbiol

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A recently described new method for determination of killer toxin activity was used for kinetic measurements of K1 toxin binding. The cells of the killer sensitive strain Saccharomyces cerevisiae S6 were shown to carry two classes of toxin binding sites differing widely in their half-saturation constants and maximum binding rates. The low-affinity and high-velocity binding component (KT1 = 2.6 x 10(9) L.U./ml, Vmax1 = 0.19 s-1) probably reflects diffusion-limited binding to cell wall receptors; the high-affinity and low-velocity component (KT2 = 3.2 x 10(7) L.U./ml, Vmax2 = 0.03 s-1) presumably indicates the binding of the toxin to plasma membrane receptors. Adsorption of most of the killer toxin K1 to the surface of sensitive cells occurred within 1 min and was virtually complete within 5 min. The amount of toxin that saturated practically all cell receptors was about 600 lethal units (L.U.) per cell of S. cerevisiae S6.

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Isolation and Properties of a Chromosome-dependentKHRKiller Toxin inSaccharomyces cerevisiae

Cited by 9 publications

References 3 publications

Whole-Genome Comparison Reveals Novel Genetic Elements That Characterize the Genome of Industrial Strains of Saccharomyces cerevisiae

Whole-Genome Comparison Reveals Novel Genetic Elements That Characterize the Genome of Industrial Strains of Saccharomyces cerevisiae

Cloning and Nucleotide Sequence of theKHSKiller Gene ofSaccharomyces cerevisiae

Kinetic studies of killer toxin K1 binding to yeast cells indicate two receptor populations

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