Isolation and properties of Aspergillus niger IBT-90 xylanase for bakery

Romanowska, Irena; Polak, Jacek; Bielecki, Stanisław

doi:10.1007/s00253-005-0011-0

Cited by 40 publications

(24 citation statements)

References 21 publications

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“…12 On the other hand, xylan acts as a major ingredient of hemicellulose in nature, so there have been a surge of interest in microbial xylanase (EC 3.2.1.8), having significance in a variety of well-documented industrial applications (especially in paper and food industries). 13 The generation of clean, sustainable energy sources from renewable carbon sources (such as lignocellulosic biomass) for the substitution of fossil fuels goes worldwide interest because the process minimizes the negative environmental and social impacts as well as the lignocellulosic resources do not compete directly with food production or with land that may be needed for the food production. 7 Among these energy alternatives, hydrogen is considered as a clean, sustainable, and renewable energy carrier, not contributing to the greenhouse effect with high energy yield of 122 MJ/ Kg.…”

Section: Introductionmentioning

confidence: 99%

Multicomponent cellulase production by Cellulomonas biazotea NCIM‐2550 and its applications for cellulosic biohydrogen production

Saratale

et al. 2009

Biotechnology Progress

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Among four cellulolytic microorganisms examined, Cellulomonas biazotea NCIM-2550 can grow on various cellulosic substrates and produce reducing sugar. The activity of cellulases (endoglucanase, exoglucanase, and cellobiase), xylanase, amylase, and lignin class of enzymes produced by C. biazotea was mainly present extracellularly and the enzyme production was dependent on cellulosic substrates (carboxymethyl cellulose [CMC], sugarcane bagasse [SCB], and xylan) used for growth. Effects of physicochemical conditions on cellulolytic enzyme production were systematically investigated. Using MnCl(2) as a metal additive significantly induces the cellulase enzyme system, resulting in more reducing sugar production. The efficiency of fermentative conversion of the hydrolyzed SCB and xylan into clean H(2) energy was examined with seven H(2)-producing pure bacterial isolates. Only Clostridiumbutyricum CGS5 exhibited efficient H(2) production performance with the hydrolysate of SCB and xylan. The cumulative H(2) production and H(2) yield from using bagasse hydrolysate (initial reducing sugar concentration = 1.545 g/L) were approximately 72.61 mL/L and 2.13 mmol H(2)/g reducing sugar (or 1.91 mmol H(2)/g cellulose), respectively. Using xylan hydrolysate (initial reducing sugar concentration = 0.345 g/L) as substrate could also attain a cumulative H(2) production and H(2) yield of 87.02 mL/L and 5.03 mmol H(2)/g reducing sugar (or 4.01 mmol H(2)/g cellulose), respectively.

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Section: Introductionmentioning

confidence: 99%

Multicomponent cellulase production by Cellulomonas biazotea NCIM‐2550 and its applications for cellulosic biohydrogen production

Saratale

et al. 2009

Biotechnology Progress

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“…The spore suspension was passed through a 0.5 mm sieve to eliminate mycelia and media components. The spore numbers were estimated by Romanowska et al (2006). Ten percent (v/v) of spore suspension (1×10 8 spore/ml) was used as inoculum for SSF process.…”

Section: Spore Propagation and Inoculum Preparationmentioning

confidence: 99%

Supplementation of Fruit Processing Waste for Endoxylanase Production by Trichoderma Koeningi Isolate and Its Optimization Using Central Composite Design: Application of Produced Endoxylanase in Mango Juice Clarification

Bandikari¹,

Mannepula²,

Poludasu³

et al. 2015

J microb biotech food sci

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The aim of this study was to optimize the supplementation of custard apple, pomegranate peel along with KH2PO4, (NH4)2SO4 and MgSO4 for maximum endoxylanase production by Trichoderma koeningi isolate on pretreated corn cobs using CCD. Predicted endoxylanase yield was highest (3242.43 U/g) with the actual yield of (3107.58U/g) from 2% NaOH pretreated corn cobs. The model predicted R2 value of 0.9147% indicating that it was appropriate to predict the levels of variables to achieve maximum endoxylanase yield. The produced endoxylanase clarified mango juice of Baneshan and Totapuri varieties at 40°C and 56 h. The endoxylanase treatment enhanced the clarity of Baneshan juice by 51.7% with a release of 121.1 mg/ml of reducing sugars at an optimized enzyme dose of 9.6 U that led to maximum flow rate of 8 ml/min. In the case of Totapuri juice, there was 43.4% increase in clarity and release of 102.6 mg/ml reducing sugars at an optimized enzyme dose of 12.8 U that lead to a maximum juice flow rate of 6 ml/min. The HPLC analysis showed that the hydrolysed products of mango juice were xylose and xylo-oligosacharides. An optimized xylanase production was achieved from low cost agro residues, which results in the reduction of enzyme cost.

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“…The disulphide bond reducing agents DTT and β-mercaptoethanol showed also an activation of other xylanases from A. giganteus 8 , A. sydowii SBS 45 11 , A. cf. niger BCC14405 9 , and A. niger IBT-90 3 . But EDTA showed a confused effect, no change in the activity of the xylanase from A. niger IBT-90 3 and the xylanase II from A. giganteus 8 , but a decrease in the activity of the xylanase from A. sydowii SBS 45 11 and the xylanase I from A. giganteus 8 .…”

Section: Effects Of Inhibitorsmentioning

confidence: 99%

“…Endoxylanases are used for pulp industry to support the bleaching process, reducing the toxic chemicals (chlorine) used to bleach lignin contained in the paper 2 . Other important applications of xylanases are to make the bread fine and soft and extend the storage time 3 ; to purify fruit juice, wine and beer 4 ; and to form xylitol glucose used in confectionery industry. In the breeding, xylanase expedites the digestion process of food containing xylan and at the same time helps to reduce the viscosity in the digestive system followed by many positive effects such as improved food absorption, improved microorganism populations of the intestine in the advantageous direction, reduced digestion disorder, and drier excrement 5,6 .…”

Section: Introductionmentioning

confidence: 99%

“…Among filamentous fungi, Trichoderma and Aspergillus have been widely studied for the xylanase production, and the genus Aspergillus is the most efficient producers of xylanolytic enzymes [7][8][9][10][11] . Thus for different applications, purification and biochemical characterization of xylanases from various Aspergillus strains have been carried out, including xylanases from A. awamori 12 , A. ficuum 10 , A. giganteus 8 , A. nidulans 7 , A. niger 3,9,[13][14][15] , and A. sydowii 11 . In the previous study, cultural conditions for xy-lanase production by an A. awamori strain VTCC-F312 were optimized in shake flask cultures 16 and we developed a nutrient medium containing 6% (w/v) fish powder and 7% (w/v) of corn cobs for optimal xylanase production.…”

Section: Introductionmentioning

confidence: 99%

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Purification and characterization of an acid-stable and organic solvent-tolerant xylanase from Aspergillus awamori VTCC-F312

Tuyen¹,

Quyen²,

Dam³

2012

ScienceAsia

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An acid-stable and organic solvent-tolerant extracellular xylanase was isolated and purified from Aspergillus awamori VTCC-F312 and its properties were investigated. The xylanase was purified by Sephadex G-100 gel filtration chromatography and DEAE-Sephadex A-50 ion exchange chromatography to homogeneity. The enzyme had a molecular mass of 32 kDa and a specific activity of 217 U/mg protein, with optimum temperature of 50-55°C and optimum pH of 5. This enzyme was stable at up to 45°C and no loss of enzyme activity was observed after incubation for 6 h at 40°C. The xylanase was stable within the pH range of 4-8 with no loss of enzyme activity after incubation at 30°C for 1-4 h, even the enzyme activity was found to increase by 60% after incubation at pH 4 for 4 h. The Km and Vmax values were 12.6 mg oat spelt xylan per ml and 1000 U/mg protein, respectively. Dithiothreitol, β-mercaptoethanol, and EDTA were found to increase the xylanase activity by 19%, 46%, and 138%, respectively. Except for Fe 3+ , the presence of 5 mM tested metal ions increased the enzyme activity by 18-52%. The enzyme showed high resistance to organic solvents and retained 63-86% and 44-61% of its activity in the presence of tested organic solvents at 30% and 80% (v/v), respectively. Triton X-100 and Tween 80 activated the xylanase to 128% and 116% of its activity, whereas SDS at the concentration of 5% completely inhibited the enzyme. These results suggested that the xylanase from A. awamori VTCC-F312 could potentially be used as a feed additive for monogastric animals.KEYWORDS: detergent-resistant, no disulphide bond, non-metal enzyme, free of cellulase and mannanase activity, corn cob, natural substrate

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Isolation and properties of Aspergillus niger IBT-90 xylanase for bakery

Cited by 40 publications

References 21 publications

Multicomponent cellulase production by Cellulomonas biazotea NCIM‐2550 and its applications for cellulosic biohydrogen production

Multicomponent cellulase production by Cellulomonas biazotea NCIM‐2550 and its applications for cellulosic biohydrogen production

Supplementation of Fruit Processing Waste for Endoxylanase Production by Trichoderma Koeningi Isolate and Its Optimization Using Central Composite Design: Application of Produced Endoxylanase in Mango Juice Clarification

Purification and characterization of an acid-stable and organic solvent-tolerant xylanase from Aspergillus awamori VTCC-F312

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