Isolation and Properties of Enterobacter sp. LX3 Capable of Producing Indoleacetic Acid

Chi, Qingshan; Tang, Wei; Liu, Lu; Meng, Jun; Dong, Xiaoli; Chen, Wenfu; Li, Xianzhen

doi:10.3390/app8112108

Cited by 14 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this work, both strains of E. casseliflavus showed no gelatin hydrolysis. Similarly, the results of no gelatin hydrolysis of Enterobacter spp., P. pentosaceus, certain A. pasteurianus, M. guilliermondii TC15, and S. cerevisiae TC6 in this work were in accordance with the previous reports showing the same species with no gelatinase activity (Fakruddin et al 2017;Chi et al 2018;Zajc et al 2019). This indicated greater potential use as probiotics since these strains are unlikely to express gelatinase, a metalloendopeptidase, which is able to degrade hemoglobin, insulin, collagen, casein, fibrinogen and gelatin (Kanemitsu et al 2001) and thus may harm the hosts.…”

Section: Discussionsupporting

confidence: 92%

Probiotic Attributes, GABA-Producing Capacity and Cytotoxic Effects of Microbes Isolated from Thai Fermented Foods

Luang-In¹

2021

IJAB

View full text Add to dashboard Cite

Mental illnesses and cancer deaths have been alarmingly increased in Thailand over the past decade. There is a search for an alternative remedy from natural sources. This work revealed the probiotic attributes of eighteen microbes from Thai fermented food sources, their neurotransmitter-producing capacities and cytotoxic effects. The probiotic attributes were comprehensively assessed by gelatin hydrolysis, antibiotic susceptibility, auto-aggregation, virulence gene detection, hemolytic activity, hydrophobicity determination, bile and acid tolerance. Neurotransmitters were produced from precursors by microbes over 3 days and analyzed by high-throughput LC-MS/MS. Cytotoxic effects of the selected microbial extracts on MCF-7, HepG2, and HeLa were determined using MTT assay. The results showed that only seven strains; Enterococcus casseliflavus 3.10A1, Enterobacter xiangfangensis 4A-2A3.1, Saccharomyces cerevisiae TC6, Pedicoccus pentosaceus WS11, Lactococcus lactis subspp. lactis TBRC 375, Lactobacillus brevis TBRC 3003 and Bifidobacterium adolescentis TBRC 7154 exhibited promising probiotic potential in every probiotic assessment. Only E. xiangfangensis 4A-2A3.1 and Bacillus spp. PS15 were capable of producing gamma-aminobutyric acid (GABA) from monosodium glutamate (20 mg/mL). GABA production of 4.60 μg/mL over 2 days was found in E. xiangfangensis 4A-2A3.1; however, Bacillus spp. PS15 produced the highest GABA at 5.57 μg/mL on day 1. Moreover, B. adolescentis TBRC 7154 extract showed the lowest IC50 values against MCF-7 (750.02 μg/mL), HepG2 (681.08 μg/mL), and HeLa (425.50 μg/mL) followed by E. xiangfangensis 4A-2A3.1, E. casseliflavus 3.10A1 and P. pentosaceus WS11. These microbes can be used as probiotic supplement with neurodegeneration preventive or chemopreventive potential. © 2021 Friends Science Publishers

show abstract

Section: Discussionsupporting

confidence: 92%

Probiotic Attributes, GABA-Producing Capacity and Cytotoxic Effects of Microbes Isolated from Thai Fermented Foods

Luang-In¹

2021

IJAB

View full text Add to dashboard Cite

show abstract

“…Using the same concentration of tryptophan as the precursor (1 mL/20 mL broth), Ng et al [45] found that Enterobacter gergoviae and Bacillus amyloliquefaciens produced IAA in the of range of 3.56-24 µg mL −1 . IAA act as a phytohormone to regulate plant growth and development, which leads to a persistent search for IAA-producing microbes for a potential application in promoting plant growth [46]. Enterobacter sp.…”

Section: Effect Of Fermentation On the Indole Acetic Acid Productionmentioning

confidence: 99%

“…Enterobacter sp. was found to have produced up to 200 mg L −1 of IAA using 1-tryptophan in NB medium [46]. The IAA production is vital in plant root elongation and root hair development [13].…”

Section: Effect Of Fermentation On the Indole Acetic Acid Productionmentioning

confidence: 99%

Fermentation of Washed Rice Water Increases Beneficial Plant Bacterial Population and Nutrient Concentrations

et al. 2021

View full text Add to dashboard Cite

Washed rice water (WRW) is said to be a beneficial plant fertilizer because of its nutrient content. However, rigorous scientific studies to ascertain its efficiency are lacking. The purpose of this study was to determine the effect of fermenting WRW on the bacterial population and identification, and to measure how fermentation affects the nutrient composition of WRW. Rice grains were washed in a volumetric water-to-rice ratio of 3:1 and at a constant speed of 80 rpm for all treatments. The treatments were WRW fermented at 0 (unfermented), 3, 6, and 9 days. Bacterial N fixation and P and K solubilization abilities in the fermented WRW were assessed both qualitatively and quantitatively. The isolated bacterial strains and the WRW samples were also tested for catalase and indole acetic acid (IAA) production ability. Significantly greater N fixation, P and K solubilization, and IAA production were recorded after 3 days of fermentation compared with other fermentation periods, with increases of 46.9–83.3%, 48.2–84.1%, 73.7–83.6%, and 13.3–85.5%, respectively, in addition to the highest (2.12 × 108 CFU mL−1) total bacterial population. Twelve bacteria strains were isolated from the fermented WRW, and the gene identification showed the presence of beneficial bacteria Bacillus velezensis, Enterobacter spp., Pantoea agglomerans, Klebsiella pneumoniae and Stenotrophomonas maltophilia at the different fermentation periods. All the identified microbes (except Enterobacter sp. Strain WRW-7) were positive for catalase production. Similarly, all the microbes could produce IAA, with Enterobacter spp. strain WRW-10 recording the highest IAA of up to 73.7% higher than other strains. Generally, with increasing fermentation periods, the nutrients N, S, P, K, Mg, NH4+, and NO3− increased, while pH, C, and Cu decreased. Therefore, fermentation of WRW can potentially increase plant growth and enhance soil health because of WRW’s nutrients and microbial promotional effect, particularly after 3 days of fermentation.

show abstract

“…Genes scrA and scrB genes in Klebsiella pneumoniae, play a role in sucrose hydrolysis (Titgemeyer et al 1996). The genus Enterobacter is a bacterium that is often found in the rhizosphere of plants, and most of them were reported to degrade sucrose (Khalifa et al 2016;Chi et al 2018). According to Wu and Birch (2005), Pantoea dispersa, as a part of ordo Enterobacterales, also produces highly efficient sucrose isomerase that can carry out two catalytic processes, hydrolysis, and isomerization of sucrose, with the final results of glucose and fructose in a ratio of 1: 1.…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification, and analysis of the invertase-producing bacteria abundance in sugarcane rhizosphere soil with different plant productivity levels

2021

View full text Add to dashboard Cite

Abstract. Lase ERK, Giyanto Santosa DA. 2021. Isolation, identification, and analysis of the invertase-producing bacteria abundance in sugarcane rhizosphere soil with different plant productivity levels. Biodiversitas 22: 3156-3162. microbes and enzymes play an important role in maintaining the stability of soil ecosystems. Invertase is one of the soil enzymes produced by microbes to hydrolyze sucrose in the environment. The aims of this study were to investigate the abundance of invertase-producing bacteria and soil invertase activity in sugarcane rhizosphere soil with different plant productivity levels and their correlation with soil chemical and physical properties and to obtain invertase-producing bacteria from the sugarcane rhizosphere. The samples of sugarcane rhizosphere soil were collected using a randomized sampling method and determine their physical-chemical properties, the abundance of invertase-producing bacteria, and soil invertase activity. Twenty invertase-producing bacteria were successfully isolated and tested their abilities to produce invertase qualitatively and quantitatively. The three best isolates (ScT112, ScT124, and ScR301) were molecularly identified using the 16S rRNA gene and phylogenetic tree analysis. The results of this study indicate that there is a significant correlation between invertase bacterial abundance and soil invertase activity. The abundance of invertase-producing bacteria correlates with pH, organic C, total N, and soil sand content. Invertase activity in the soil correlates with pH and organic C. Based on the phylogenetic tree, the isolates of ScT112 had the closest homology with Cupriavidus sp., ScT124 was homologous with Klebsiella variicola, while ScR301 was homologous with Pantoea sp. These three isolates have the potential to be developed in industrial biotechnology to produce invertase. The hydrolysis zones of ScT112, ScT124, and ScR301 were 3.12 cm, 2.76 cm, and 2.55 cm, respectively with the invertase activity of 27.82 U mL-1, 24.56 U mL-1, and 24.08 U mL-1.

show abstract

Isolation and Properties of Enterobacter sp. LX3 Capable of Producing Indoleacetic Acid

Cited by 14 publications

References 24 publications

Probiotic Attributes, GABA-Producing Capacity and Cytotoxic Effects of Microbes Isolated from Thai Fermented Foods

Probiotic Attributes, GABA-Producing Capacity and Cytotoxic Effects of Microbes Isolated from Thai Fermented Foods

Fermentation of Washed Rice Water Increases Beneficial Plant Bacterial Population and Nutrient Concentrations

Isolation, identification, and analysis of the invertase-producing bacteria abundance in sugarcane rhizosphere soil with different plant productivity levels

Contact Info

Product

Resources

About