Isolation and purification of apigenin, quercetin and apigenin 7-O-glycoside from Apium graveolens L., Petroselinum crispum (Mill.) Fuss, Allium cepa L., respectively

Tavakoli, Sina; Khalighi-Sigaroodi, Farahnaz; Dehaghi, Nafiseh Khosravi; Yaghoobi, Mahdi; Hajiaghaee, Reza; Gholami, Ahmad; Ghafarzadegan, R

doi:10.52547/jmp.21.83.72

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…A yellow powder, compound C4 (also known as quercetin) was obtained in a quantity of 25 mg. Its behavior in various solvent systems was analyzed through chromatography on PC, which showed that it is an aglycone (with R f values of 0.69 in BAW and 0.21 in 15% acetic acid). The spectra measured for the compounds were consistent with those reported for quercetin in a previous study conducted by [ 27 ].…”

Section: Resultssupporting

confidence: 88%

“…1 H-NMR analysis showed peaks at δ ppm 7.92 (2H,d, J = 8.2 Hz H-2,6), 6.90 (2H, d, J = 8.15 Hz, H-3', H-5'), 6.82 (1H, d, J=2.2 Hz, H-8), 6.43(1H, d, J= 2.2 Hz, H-6), 5.44 (1H, d, J=7.4 Hz, H-1"), and 3.17-3.47 (5H, m, H-3"–H-6"). The data matched the characteristics reported in the literature [ 27 ]. The compound C8, also known as kaempferol-3,7- O -dirhamnoside, is a yellow amorphous powder with a weight of 16 mg and a yield of 25 mg.…”

Section: Resultssupporting

confidence: 81%

“…A yellow shapeless compound C3, was found and its R f values were measured in two solvents, 15% acetic acid and BAW (3:1:1), showing that it is an aglycone. The data gathered from UV, MS, and 1 H-NMR analyses were identical to the data reported for apigenin in a previous study by [ 27 ].…”

Section: Resultssupporting

confidence: 75%

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Phytochemical analysis and evaluation of its antioxidant, antimicrobial, and cytotoxic activities for different extracts of Casuarina equisetifolia

Abdallah,

Shams,

El-Shamy

2024

BMC Complement Med Ther

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Background Casuarina equisetifolia belongs to the Casuarina species with the most extensive natural distribution, which contain various phytochemicals with potential health benefits. This study aimed to investigate the chemical composition and biological activities of different extracts of Casuarina equisetifolia. Methods The n-hexane extract was analyzed for its unsaponifiable and fatty acid methyl esters fractions, while chloroform, ethyl acetate, and butanol extracts were studied for their phenolic components. Six different extracts of C. equisetifolia needles were evaluated for their total phenolic content, total flavonoid content, and their antioxidant, antimicrobial, and cytotoxic activities. Results The n-hexane extract contained mainly hydrocarbons and fatty acid methyl esters, while ten phenolic compounds were isolated and identified in the chloroform, ethyl acetate, and butanol extracts. The methanolic extract exhibited the highest total phenolic and flavonoid content, highest antioxidant activity, and most potent cytotoxic activity against HepG-2 and HCT-116 cancer cell lines. The ethyl acetate extract showed the most significant inhibition zone against Staphylococcus aureus and Bacillus subtilis. Conclusion Casuarina equisetifolia extracts showed promising antioxidant, antimicrobial, and cytotoxic activities. Overall, Casuarina equisetifolia is a versatile tree with a variety of uses, and its plant material can be used for many different purposes.

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Section: Resultssupporting

confidence: 88%

Section: Resultssupporting

confidence: 81%

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Phytochemical analysis and evaluation of its antioxidant, antimicrobial, and cytotoxic activities for different extracts of Casuarina equisetifolia

Abdallah,

Shams,

El-Shamy

2024

BMC Complement Med Ther

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Bioefficacy, chromatographic profiling and drug-likeness analysis of flavonoids and terpenoids as potential inhibitors of H1N1 influenza viral proteins

Libin,

Debnath,

Sisodiya

et al. 2024

International Journal of Biological Macromolecules

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Synergistic effect of phenylpropanoids and flavonoids with antibiotics against Gram-positive and Gram-negative bacterial strains

Kincses,

Ghazal,

Hohmann

2024

Pharmaceutical Biology

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Context The increase in bacterial resistance to currently available medications, which increases mortality rates, treatment costs is a global problem, and highlights the need for novel classes of antibacterial agents or new molecules that interact synergistically with antimicrobials. Objective The current work explores the potential synergistic effects of certain natural phenylpropanoids and flavonoids on ciprofloxacin (CIP), ampicillin (AMP), gentamicin (GEN), and tetracycline (TET). Materials and methods The adjuvant role of cinnamic acid, p -coumaric acid, caffeic acid, ferulic acid, ferulic acid methyl ester, sinapic acid, apigenin, and luteolin was evaluated by determining the MIC (minimal inhibitory concentration) values of antibiotics in the presence of subinhibitory concentrations (200, 100, and/or 50 µM) of the compounds in Gram-positive and Gram-negative bacterial strains using a 2-fold broth microdilution method. The 96-well plates were incubated at 37 °C for 18 h, and dimethyl sulfoxide was used as a solvent control. Results The combination of luteolin with CIP, reduced the MIC values of the antibiotic from 0.625 to 0.3125 µM and to 0.078 µM in 100 and 200 µM concentration, respectively, in sensitive Staphylococcus aureus . Sinapic acid decreased the MIC value of CIP from 0.625 to 0.3125 µM in S. aureus , from 1.56 to 0.78 µM in Klebsiella pneumoniae , and the MIC of GEN from 0.39 to 0.095 µM in Pseudomonas aeruginosa strains. Discussion and conclusions These findings are useful in delaying the development of resistance, as the required antibacterial effect can be achieved with the use of lower concentrations of antibiotics.

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Isolation and purification of apigenin, quercetin and apigenin 7-O-glycoside from Apium graveolens L., Petroselinum crispum (Mill.) Fuss, Allium cepa L., respectively

Cited by 4 publications

References 18 publications

Phytochemical analysis and evaluation of its antioxidant, antimicrobial, and cytotoxic activities for different extracts of Casuarina equisetifolia

Phytochemical analysis and evaluation of its antioxidant, antimicrobial, and cytotoxic activities for different extracts of Casuarina equisetifolia

Bioefficacy, chromatographic profiling and drug-likeness analysis of flavonoids and terpenoids as potential inhibitors of H1N1 influenza viral proteins

Synergistic effect of phenylpropanoids and flavonoids with antibiotics against Gram-positive and Gram-negative bacterial strains

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