Isolation and Purification of metalloprotease produced by Aeromonas hydrophila

Abdallah, Muhannad Moeen; Saleh, Tahreer Hadi

doi:10.26505/djm.14023660924

Cited by 1 publication

(2 citation statements)

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“…This process is characterized by the activation of different types of caspase enzymes and contributes to the activation of pro-inflammatory cytokines, recruits more immune cells, and finally activates an inflammatory cascade in the tissue (14). In the last years, few studies have investigated the expression of several innate immune-related genes against A. hydrophila, A. veronii, and Aeromonas salmonicida in different cell lines or animal models (mice and fish) (12,(21)(22)(23)(24). The majority of the studies has been performed with cell lines obtained from animals, with the exception of two cell lines (T-84 and WLR-68) obtained from the human colon (12,24).…”

Section: Introductionmentioning

confidence: 99%

“…In the last years, few studies have investigated the expression of several innate immune-related genes against A. hydrophila, A. veronii, and Aeromonas salmonicida in different cell lines or animal models (mice and fish) (12,(21)(22)(23)(24). The majority of the studies has been performed with cell lines obtained from animals, with the exception of two cell lines (T-84 and WLR-68) obtained from the human colon (12,24). However, human immune-related cell lines have, so far, not been used as cell models in the Aeromonas studies.…”

Section: Introductionmentioning

confidence: 99%

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Immune Response of the Monocytic Cell Line THP-1 Against Six Aeromonas spp.

Fernández-Bravo

Figueras

2022

Front. Immunol.

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Aeromonas are autochthonous bacteria of aquatic environments that are considered to be emerging pathogens to humans, producing diarrhea, bacteremia, and wound infections. Genetic identification shows that 95.4% of the strains associated with clinical cases correspond to the species Aeromonas caviae (37.26%), Aeromonas dhakensis (23.49%), Aeromonas veronii (21.54%), and Aeromonas hydrophila (13.07%). However, few studies have investigated the human immune response against some Aeromonas spp. such as A. hydrophila, Aeromonas salmonicida, and A. veronii. The present study aimed to increase the knowledge about the innate human immune response against six Aeromonas species, using, for the first time, an in vitro infection model with the monocytic human cell line THP-1, and to evaluate the intracellular survival, the cell damage, and the expression of 11 immune-related genes (TLR4, TNF-α, CCL2, CCL20, JUN, RELA, BAX, TP53, CASP3, NLRP3, and IL-1β). Transcriptional analysis showed an upregulated expression of a variety of the monocytic immune-related genes, with a variable response depending upon the Aeromonas species. The species that produced the highest cell damage, independently of the strain origin, coincidentally induced a higher expression of immune-related genes and corresponded to the more prevalent clinical species A. dhakensis, A. veronii, and A. caviae. Additionally, monocytic cells showed an overexpression of the apoptotic and pyroptotic genes involved in cell death after A. dhakensis, A. caviae, and Aeromonas media infection. However, the apoptosis route seemed to be the only way of producing cell damage and death in the case of the species Aeromonas piscicola and Aeromonas jandaei, while A. veronii apparently only used the pyroptosis route.

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