Isolation and Purification of Protein That Is Immunologically Similar to Human Lactoferrin

Abstract

The study aimed at isolating a substance that is immunologically similar to human lactoferrin, hereinafter — microbial-derived lactoferrin (MdLF) isolated from K.pneumoniae liquid culture. Protein extraction started with isolation of ballast proteins in 2M ammonium sulphate (p.a.). Ion chromatography with cation-exchange agents was the basic method used for isolating MLF. Proteins from the isolated protein fractions were identified with AGID method. The isolated MLF is glucoprotein with the molecular mass of about 84,000, the agarose diffusion coefficient of 3.1 × 10–7 cm2 sec–1 and the relative electrophoretic mobility of 0.42. MLF is characterized by low hydrophobicity and elutes from phenylSepharose with 0.4 ammonium sulphate. Its isoelectric point equals to 9.2.

Role of Microbial Factor in Male Infertility Pathogenesis. Literature Review

“…Riegel et al isolated a new species of pathogenic corynebacteria, C.seminale sp. nov, from the semen of infertile men [21,22,23,24]. Therefore, it is obvious that representatives of human autoflora are the main pathogens of male urogenital infections that result in pathospermia development.…”