Isolation and purification of proteoglycans

Fedarko, Neal S.

doi:10.1007/978-3-0348-7545-5_3

Cited by 9 publications

(7 citation statements)

References 140 publications

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“…As a result, the PLA must resort to foreign currency so that it can purchase western technology and equipment, such as American gas turbine engines that may be used to extend the range and payload capacity of the Silkworm anti-ship cruise missile. 42 With the ballistic missile becoming the delivery vehicle of choice in the third world, the PLA is likely to compensate for its shortage of funds through increased sales of missile technology to almost any customer with little or no regard for regional stability.…”

Section: The Pla and Defence Modernizationmentioning

confidence: 99%

The dynamics of Chinese missile proliferation

Mullins

1995

The Pacific Review

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The missile nonproliferation regime has been criticized for its ineffectiveness in constraining states such as China from contributing to the proliferation of ballistic missile systems and related technologies to states in other regions. This regime is predicated on the assumption that the creation of social norms and acceptable patterns of behaviour will ultimately require China and others to conform to a universal perpective on the sources of and remedies for missile proliferation. The purpose of this article is to demonstrate that China's willingness to supply sensitive missile technology is a function of a weapons-export policy that is incompatible with current initiatives in missile nonproliferation. Such a policy is the product of certain foreign policy considerations and domestic factors that serve as motivational elements within the internal political system.

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Section: The Pla and Defence Modernizationmentioning

confidence: 99%

The dynamics of Chinese missile proliferation

Mullins

1995

The Pacific Review

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“…The columns are generally equilibrated in urea-containing buffers (e.g. 7M urea, 0.05 M sodium acetate, pH 6-7, 0.5% detergent) and eluted with NaCl gradients [49]. Strong anion-exchange chromatographic supports containing quaternary ammonium groups have also been used (Mono Q [47], Q-Sepharose [39,50]).…”

Section: Purificationmentioning

confidence: 99%

“…Electrostatic interactions occur between the cationic sites of the mineral (calcium) and the anionic groups of the analyte (carboxyl and sulfate). For chromatography, most columns are equilibrated by extensive washing with phosphate buffer at pH 6.8 [49], which confers a negative net charge to the resin's surface. Carboxyl groups on the protein core or GAGs are repelled electrostatically from the negatively charged phosphate groups of the column and bind by complexing to calcium.…”

Section: Purificationmentioning

confidence: 99%

Recent Developments in Proteoglycan Purification and Analysis

Didraga¹,

Barroso²,

Bischoff³

2006

CPA

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Proteoglycans are ubiquitous biomolecules in the body located in the extracellular matrix, on the cell surface and also within the cells. They contain at least one glycosaminoglycan (GAG) chain covalently attached to a core protein and may also present N-or O-linked glycans. The high structural diversity and distribution relate to the various biological functions of proteoglycans. In recent years, new members have enlarged the proteoglycan family and advances in molecular biology and glycobiology contributed to elucidate more of the biological functions of proteoglycans. In order to study the structure of a proteoglycan molecule and relate it to its function (or dysfunction), its isolation and purification from cell culture or tissue extracts is necessary. Next to the widely used anion exchange chromatographic methods, techniques based on lectin affinity chromatography have created new possibilities to increase the degree of purity. Introduction of electrospray ionization (ESI) and matrix-assisted laser desorption-ionization (MALDI) sources, together with tandem mass spectrometry (MS/MS or MS n ), mark a further important step towards the structural analysis of glycosaminoglycans. The aim of this review is to present the most recent advances in proteoglycan purification and analysis.

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“…The preparations obtained by SEC were characterized by a combination of ESI-MS, MALDI-TOF-MS, and fluorophore-assisted carbohydrate electrophoresis. Isolation and purification of proteoglycans by SEC was the subject of a review by Fedarko [350]. It was demonstrated that formation of the multiply charged oligomers that bind up to five sodium cations permits the rapid analysis of polysaccharides with molecular masses in excess of 9 kDa.…”

Section: Carbohydratesmentioning

confidence: 99%