Isolation and Purification of Sesquiterpene Lactones from <i>Ixeris sonchifolia</i> (Bunge) Hance by High-Speed Counter- Current Chromatography and Semi-Preparative High Performance Liquid Chromatography

Abstract

Purpose: To isolate and purify sesquiterpene lactones from Ixeris sonchifolia (Bunge) Hance by highspeed counter-current chromatography (HSCCC).Methods: I. sonchifolia was extracted with water and then loaded on a glass column (10 × 1500 cm containing 3000g D101 macroporous resin) where various concentrations of aqueous ethanol (0, 10, 30, 50, and 95 %) were used to elute the column successively. The 50 % ethanol fraction was purified by HSCCC using a solvent system comprised of ethyl acetate : n-butanol: methanol: water (4: 6: 1: 20, v/v) , and semi-preparative high performance liquid chromatography (HPLC). The chemical structures of the components obtained were further confirmed by high-resolution mass spectroscopy (MS) and nuclear magnetic resonance spectroscopy (NMR).Results: Three compounds, including ixerin Z1 (0.7 mg), ixerin Z (11.4 mg),and 11, with purity of 96.2, 98.2, and 98.4 %, respectively, were

Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3‐L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro

“…[22] [23] Considering the advantages of macroporous resin columns and semipreparative RPLC, these two techniques were combined in this study to isolate the target compound. However, efficient preparative separation and purification methods for natural compounds are needed.…”

“…Semi-preparative reversed-phase liquid chromatography (semi-preparative RPLC) is gradually becoming a powerful tool by virtue of its high efficiency and resolution. [22] [23] Considering the advantages of macroporous resin columns and semipreparative RPLC, these two techniques were combined in this study to isolate the target compound. Macroporous resin columns were mainly used to enrich the effective fraction and semi-preparative RPLC was used to separate the target compounds.…”