Isolation and recovery of glycomacropeptide from milk whey by means of thermal treatment

Abstract

During enzymatic process of cheese manufacturing, rennin cleaves κ-casein releasing two fractions: para-κ-casein and glycomacropeptide (GMP), which remains soluble in milk whey. GMP is a peptide with structural particularities such as chain carbohydrates linked to specific threonine residues, to which a great variety of biological activities is attributed. Worldwide cheese production has increased generating high volumes of milk whey that could be efficiently used as an alternative source of high quality peptide or protein in foodstuff formulations. In order to evaluate isolation and recovery on whey GMP by means of thermal treatment (90 °C), 18 samples (2 L each) of sweet whey, resuspended commercial whey (positive control) and acid whey (negative control) were processed. Indirect presence of GMP was verified using chemical tests and PAGE-SDS 15%. At 90 °C treated sweet whey, 14, 20 and 41 kDa bands were observed. These bands may correspond to olygomers of GMP. Peptide recovery showed an average of 1.5 g/L (34.08%). The results indicate that industrial scale GMP production is feasible; however, further research must be carried out for the biological and nutritional evaluation of GMP's incorporation to foodstuff as a supplement.

Enhancing the activity of biocatalysts supported on calcium phosphate by inducing mesoporosity with phosphopeptides

“…Polymeric CGMP (Poly-CGMP) was then prepared from this monomer by enzymatic crosslinking with microbial transglutaminase (mTG), following a procedure previously reported (see SI1) [14][15][16][17][18]. The enzyme (Enz), a lactase (Trade name Ha-Lactase, which activity is of 5200 NLU•g -1 of solution, namely 100k NLU•g -1 of protein), was provided by Chr.…”

Molecular Insights Into O-Linked Glycan Utilization by Gut Microbes

“…The molecular weight of GMP ranges from 6.7 to 8 kDa (Córdova-Dávalos et al, 2019). GMP is available in large quantities in dairy streams (Rojas and Torres, 2013).…”

Glycomacropeptide Bioactivity and Health: A Review Highlighting Action Mechanisms and Signaling Pathways

“…For this reason, its use as a source of protein in phenylketonuria (PKU) patients was proposed [9], although further studies are necessary to prove its efficacy [10]. Due to the absence of aromatic amino acids, it is only detected at a wavelength of 205 to 217 nm [11,12]. Furthermore, GMP consists of only one residue of methionine and is rich in branched-chain amino acids (leucine, isoleucine, valine) [3,8].…”