Isolation and scale-up of sodium pyruvate from fermentation broth using a repulsive extraction and crystallization method

Ingle, U. M.; Rajagopalan, S.; Padmanabhan, Sasisanker

doi:10.1002/jctb.4539

Cited by 11 publications

(3 citation statements)

References 30 publications

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“…The approach involves selectively reducing the solubility of the desired product. Acetone is the most effective extractant at reducing the solubility of pyruvate from the fermentation broth, and integrating acetone extraction with microfiltration, evaporation (to concentrate the mixture) and crystallization led to 97% pyruvate purity and 75% recovery [102].…”

Section: Downstream Processesmentioning

confidence: 99%

Recent Progress in the Microbial Production of Pyruvic Acid

Maleki

Eiteman

2017

Fermentation

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Pyruvic acid (pyruvate) is a cellular metabolite found at the biochemical junction of glycolysis and the tricarboxylic acid cycle. Pyruvate is used in food, cosmetics, pharmaceutical and agricultural applications. Microbial production of pyruvate from either yeast or bacteria relies on restricting the natural catabolism of pyruvate, while also limiting the accumulation of the numerous potential by-products. In this review we describe research to improve pyruvate formation which has targeted both strain development and process development. Strain development requires an understanding of carbohydrate metabolism and the many competing enzymes which use pyruvate as a substrate, and it often combines classical mutation/isolation approaches with modern metabolic engineering strategies. Process development requires an understanding of operational modes and their differing effects on microbial growth and product formation.

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Section: Downstream Processesmentioning

confidence: 99%

Recent Progress in the Microbial Production of Pyruvic Acid

Maleki

Eiteman

2017

Fermentation

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“…But the operation process is complex. Reactive extraction, was used to separate PYR as reported by many researchers in recent years . Pal et al reported the isolation of PYR with low‐cost diluents and a final recovery yield of 62% .…”

Section: Introductionmentioning

confidence: 99%

“…Reactive extraction, was used to separate PYR as reported by many researchers in recent years. 13,14 Pal et al reported the isolation of PYR with lowcost diluents and a final recovery yield of 62%. 15 However, reactive extraction has not been reported to be used for the separation of KGA and PYR from fermentation broth.…”

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confidence: 99%

Separation of α‐ketoglutaric acid and pyruvic acid from the culture broth of Yarrowia lipolytica WSH‐Z06 by chromatographic methods

Peng

Zeng

et al. 2018

Biotechnology Progress

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Both α‐ketoglutaric acid (KGA) and pyruvic acid (PYR) are important keto acids. Efficient co‐production of KGA and PYR has been achieved in our previous work, and could significantly decrease the cost of fermentation production. KGA and PYR have similar physical and chemical properties. Hence, finding a way to separate the two keto acids efficiently has become a key challenge. In this study, different chromatographic methods have been investigated, including ion‐exchange chromatography, aluminum oxide chromatography and silica gel chromatography. The results show that the two keto acids can be well separated with silica gel chromatography, whereas ion‐exchange chromatography and aluminum oxide chromatography could not separate them. Using the pretreated fermentation broth of Yarrowia lipolytica WSH‐Z06, the purity and yield of KGA/PYR reached 98.7%/99.1% and 86.7%/70.9%, respectively, with an optimized silica gel chromatography‐based procedure. This study provides an efficient method for separating KGA and PYR from fermentation broth, which might be applied on an industrial scale and significantly decrease the cost of biotechnological production of keto acids. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1370–1379, 2018

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Metabolites recovery from fermentation broths via pressure‐driven membrane processes

Díaz‐Montes

Castro‐Muñoz

2019

Asia-Pacific J Chem Eng

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The production of specific metabolites using microorganism has been promoted and enhanced by two different approaches, such as modification of the metabolic pathways of microorganism and optimization of culture medium conditions to synthesize specific compounds. However, research community is today focused on the development of processes and techniques for a suitable recovery of metabolites from different stages of fermentation. For this purpose, the use of organic solvents has been the most common approach for the recovery of specific target compounds according to the solvent affinity. Additionally, these techniques also require the implementation of unit processes to obtain better recovery rates; it means that solvent extraction methods need the implementation of additional steps to recover the solvents used, which may involve the increase of final costs in the production process. For this reason, researchers have started to consider membrane-based technologies (e.g. microfiltration [MF], ultrafiltration [UF], and nanofiltration [NF]), as an alternative for the recovery of metabolites from fermentation broths. Therefore, the objective of this paper is to provide an overview of the current findings of the recovery of metabolites from fermentation broths by means of pressuredriven membrane processes. Particular attention will be paid to relevant data, analyzing and discussing according to the membrane features, metabolite properties, and some other phenomena that influence in the separation.Moreover, a framework of the application of the MF, UF, and NF processes in solutes recovery is addressed. Finally, some fundamentals of these processes are also given.

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Isolation and scale-up of sodium pyruvate from fermentation broth using a repulsive extraction and crystallization method

Cited by 11 publications

References 30 publications

Recent Progress in the Microbial Production of Pyruvic Acid

Recent Progress in the Microbial Production of Pyruvic Acid

Separation of α‐ketoglutaric acid and pyruvic acid from the culture broth of Yarrowia lipolytica WSH‐Z06 by chromatographic methods

Metabolites recovery from fermentation broths via pressure‐driven membrane processes

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