Isolation and Screening of Amylase Producing Fungi

Reddy, D. Vishnu Vardhan; Gnanaprakash, D. Sreenivas M.; Harikrishna, Ch.

doi:10.20546/ijcmas.2017.604.098

Cited by 7 publications

(2 citation statements)

References 5 publications

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“…Firstly, most of the proteins in Tables 1 and 2, including CtCel5E were recombinant while proteins in our own extract were not. Secondly, the source of some of the proteins, including CtCel5E was bacterial or prokaryotic whilst that of our own was fungal or eukaryotic, of which fungi are naturally known to be superior producers of lignocellulolytic enzymes (Favaro et al, 2013;Ramanjaneyulu et al, 2015). Lastly and in the event that substrate concentration was a limiting factor in the study, the cellulose content of most lignocellulosic substrates is always higher than that of hemicellulose, e.g., wheat straw, rice straw, switch grass and sugarcane bagasseall have around 35% cellulose and at most 25% hemicellulose content (Chen, 2014;Koshy and Nambisan, 2012;Shawky et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

The potential use of Lentinus edodes to manage and control water hyacinth in Zimbabwe

Sibanda¹,

Ruzvidzo²,

Zvidzai³

et al. 2020

J. Yeast Fungal Res.

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The rapid expansion and reproduction of certain plant species represents one of the biggest problems in aquatic environments, ranging from eutrophication to the limited availability of water for human consumption. Among these plants is water hyacinth (Eichhornia crassipes), a herbaceous hydrophyte often branded the world's worst aquatic weed due to its invasive aggression, negative impact on aquatic environments, and the cost usually associated with its management. Water hyacinth is a biomass, typically rich in lignocellulosic material and making it a potential raw material for the synthesis of products of industrial and domestic interest; e.g. edible fungi. Among the commonly known edible fungi is Lentinus edodes, a commercial mushroom whose versatile nature as a white rot fungus provides basis for the continued exploration of its biochemical processes during solid state fermentation on various lignocellulosic biomass as potential substrates. The fungus naturally feeds on lignocellulose by secreting various extracellular enzymes responsible for breaking down this organic polymer into simple soluble molecules that the hyphae can absorb and develop into mycelia. In this study, L. edodes was assessed for its ability to grow on water hyacinth and possibly utilizing it as a substrate. When cultured onto this noxious biomass followed by assessment by agar plate-based clearing assay and spectrophotometry, the fungus demonstrated its ability to secrete cellulases, xylanases, pectinases, peroxidases and laccases, thus showing its capabilities to physiologically utilize this hydrophyte as a substrate. If properly optimized, this approach can be remarkably used as a sustainable way to control water hyacinth in Zimbabwe.

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Section: Discussionmentioning

confidence: 99%

The potential use of Lentinus edodes to manage and control water hyacinth in Zimbabwe

Sibanda¹,

Ruzvidzo²,

Zvidzai³

et al. 2020

J. Yeast Fungal Res.

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“…amyloglucosidase; Exo-1,4-αglucosidase; lysosomal α-glucosidase; 1,4-α-D-glucan glucohydrolase) hydrolyzes α-1,4 glycosidic bonds from the non-reducing ends of starch, resulting in the production of glucose and to a lesser extent, it also has the ability to hydrolyze α-1,6 linkages, also resulting in glucose as the end-product [4][5][6]. As one of the major stakeholder, amylase constitute a class of industrial enzymes having around 25%-33% of the enzyme share in the market [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Molecular and Biochemical Characterization of a Salt Tolerant α-Amylase Producing Isolate NH-25

Ahmad

Zohra

Qader

2018

J. Basic Appl. Sci.

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Halophiles are considered as important sources of extremozymes that are not only salt tolerant but can catalyze reactions efficiently under harsh conditions of various industrial processes. Among industrially important enzymes alpha amylase is used in many industries such as starch, pharmaceutical, detergent, paper, food, and textile. Due to wide applications there is a continuous need for novel sources of the enzyme to be discovered. One of the new variant from various amylase producing isolates of marine samples of Arabian Sea, an industrial dumping site and a rich natural source, particularly for microbes, a salt tolerant α-amylase producer was studied. This isolate based on cultural characteristics, biochemical tests and molecular characterization inferred from 16s rDNA sequencing identified as Bacillus subtilis NH-25.

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Isolation and Enzymatic Characterization of Fungal Strains from Grapevines with Grapevine Trunk Diseases Symptoms in Central Mexico

Argüelles-Moyao,

Ángeles-Argáiz,

Garibay-Orijel

et al. 2024

Curr Microbiol

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Isolation and Screening of Amylase Producing Fungi

Cited by 7 publications

References 5 publications

The potential use of Lentinus edodes to manage and control water hyacinth in Zimbabwe

The potential use of Lentinus edodes to manage and control water hyacinth in Zimbabwe

Molecular and Biochemical Characterization of a Salt Tolerant α-Amylase Producing Isolate NH-25

Isolation and Enzymatic Characterization of Fungal Strains from Grapevines with Grapevine Trunk Diseases Symptoms in Central Mexico

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