Isolation and Screening of Antibiotics Producing Streptomyces spp from the Soil Collected around the Root of Alnus nepalensis from Godawari

Abstract

Actinomycetes are considered as the most invaluable prokaryotes whose genome mining show a great number of putative secondary metabolite biosynthesis pathways as well as gene clusters ranging from 20 to 50 per genome. The genus Streptomyces has been explored for its ability to produce 60% antibiotics worldwide. Alnus nepalensis (Alder) has been found to harbor diverse Eubacteria in its rhizosphere. To evaluate the antibiotic production potential from Actinomycetes, we collected soil samples from rhizosphere (5-7 cm deep) of Alder tree. Primary screening was done by cross-streak method against Multidrug Resistant (MDR) such as Methicillin resistant Staphylococcus auereus (MRSA), Vancomycin resistant Enterococcus feacalis (VRE), Imepenem resistant Acinetobacterbaumannii, Vancomycin resistant Klebsiella pneumonia and Imepenem resistant E. coli as well as Non-MDRs (E. coli, Bacillus subtilis, Klebsiella pneumoniae, S. aeureu and Enterococcus feacalis). Extraction of antibiotics was done using rota-vapour from extract obtained by solid-substrate fermentation technique followed by solvent extraction. Secondary screening was done using well diffusion assay against MDRs. Among total of 40 isolates of Actinomycetes recovered, 14 showed remarkable zone of inhibition (ZOI) to various MDRs. NASA 303 showed 26 mm of ZOI against VRE, NASA 101 had ZOI of 34 mm against MRSA, NASA 319 had 33.7 mm ZOI against Imepenem resistant E. coli, NASA 306 had 36 mm of ZOI against Vancomycin resistant Klebsiella pneumoniae, and NASA 108 showed ZOI of 29.6 mm against Imepenem resistant E. faecalis. This investigation revealed that the Actinomycetes found in Rhizosphere of Alder tree had MDR killing potent antibiotics, which needs to be further explored.

Isolation, Characterization, and Screening of Antimicrobial-Producing Actinomycetes from Soil Samples

“…Researchers have done many studies on isolation and screening of antimicrobial-producing actinomycetes. It has propounded that most of the novel antibiotics have been found by screening of isolates from soil [6]. However, due to the emergence of multidrug-resistant pathogens, antimicrobial resistant is on the rise, which is pernicious to the health of the large population of community.…”

Fermentation and Extraction of Antibacterial Metabolite Using Streptomyces spp. Isolated from Taplejung, Nepal

“…There is an urgent need to investigate alternative treatment options while there are still a few antibiotics left. New resistance mechanisms emerge and spread globally threatening the treatment of common infectious diseases, resulting in more deaths and disabilities (Baniya et al, 2018).…”

“…Researchers have done numerous studies on the isolation and screening of antimicrobial-producing actinomycetes. It has been propounded that most of the novel antibiotics was found with screening of isolates from soil (Baniya et al, 2018).…”

Characteristics Changes on Applications of Antibiotics and Current Approaches to Enhance Productivity with Soil Microbiome

“…However, these traditional methods of isolation and screening helped in discovery of novel antibiotics on soil microflora. 16 Nevertheless, due to mutation of microbiota various multidrug-resistant pathogens started to emerge which is insidious to the human population health. Therefore, it has been regarded as a perilous problem for therapy of infectious malady.…”