Isolation and screening of potential actinobacteria for rapid composting of rice straw

Kausar, H.; Meon, Sariah; Saud, Halimi Mohd; Alam, Md. Zahangir; Ismail, Mohd Razi

doi:10.1007/s10532-010-9407-3

Cited by 70 publications

(35 citation statements)

References 33 publications

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“…Enrichment of Micromonospora under thermophilic conditions in this study is consistent with these prior observations. Moreover, several Micromonospora species have been shown to be capable of deconstructing rice straw in liquid culture and compost systems [48]–[50]. These data support the possibility that enrichment of Micromonospora species in this study under thermophilic conditions corresponds to these species taking a more active role in rice straw deconstruction compared to mesophilic conditions.…”

Section: Discussionsupporting

confidence: 84%

Discovery of Microorganisms and Enzymes Involved in High-Solids Decomposition of Rice Straw Using Metagenomic Analyses

et al. 2013

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High-solids incubations were performed to enrich for microbial communities and enzymes that decompose rice straw under mesophilic (35°C) and thermophilic (55°C) conditions. Thermophilic enrichments yielded a community that was 7.5 times more metabolically active on rice straw than mesophilic enrichments. Extracted xylanase and endoglucanse activities were also 2.6 and 13.4 times greater, respectively, for thermophilic enrichments. Metagenome sequencing was performed on enriched communities to determine community composition and mine for genes encoding lignocellulolytic enzymes. Proteobacteria were found to dominate the mesophilic community while Actinobacteria were most abundant in the thermophilic community. Analysis of protein family representation in each metagenome indicated that cellobiohydrolases containing carbohydrate binding module 2 (CBM2) were significantly overrepresented in the thermophilic community. Micromonospora, a member of Actinobacteria, primarily housed these genes in the thermophilic community. In light of these findings, Micromonospora and other closely related Actinobacteria genera appear to be promising sources of thermophilic lignocellulolytic enzymes for rice straw deconstruction under high-solids conditions. Furthermore, these discoveries warrant future research to determine if exoglucanases with CBM2 represent thermostable enzymes tolerant to the process conditions expected to be encountered during industrial biofuel production.

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Section: Discussionsupporting

confidence: 84%

Discovery of Microorganisms and Enzymes Involved in High-Solids Decomposition of Rice Straw Using Metagenomic Analyses

et al. 2013

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“…They have incredible metabolic versatility, and their spores are highly resistant to desiccation stress. Although Micromonospora is not among the genera that are usually mentioned among the microorganisms that break down hydrocarbons, in previous studies, they were well documented as efficient lignocellulose (Kausar et al 2011), pesticide (Fuentes et al 2010) and hydrocarbon (Arafa 2003) degraders.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study

et al. 2011

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This article presents a study of the efficiency and degradation pattern of samples of petroleum sludge and polluted sandy soil from an oil refinery. A bacterial consortium, consisting of strains from the genera Pseudomonas, Achromobacter, Bacillus and Micromonospora, was isolated from a petroleum sludge sample and characterized. The addition of nitrogen and phosphorus nutrients and a chemical surfactant to both the samples and bioaugmentation to the soil sample were applied under laboratory conditions. The extent of biodegradation was monitored by the gravimetric method and analysis of the residual oil by gas chromatography. Over a 12-week experiment, the achieved degree of TPH (total petroleum hydrocarbon) degradation amounted to 82-88% in the petroleum sludge and 86-91% in the polluted soil. Gas chromatography-mass spectrometry was utilized to determine the biodegradability and degradation rates of n-alkanes, isoprenoids, steranes, diasteranes and terpanes. Complete degradation of the n-alkanes and isoprenoids fractions occurred in both the samples. In addition, the intensities of the peaks corresponding to tricyclic terpenes and homohopanes were decreased, while significant changes were also observed in the distribution of diasteranes and steranes.

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“…The cultures were harvested by centrifuging at 10,000 rpm for 10 min. Pellets were suspended in NB and bacterial suspension was adjusted to 10 8 cfu•mL -1 (Kausar et al, 2011) for plant inoculation.…”

Section: Inoculums Preparationmentioning

confidence: 99%

Molecular Characterization of Stress Tolerant Plant Growth Promoting Rhizobacteria (PGPR) for Growth Enhancement of Rice

Habib¹,

Kausar²,

Saud³

et al. 2015

IJAB

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The study was undertaken to characterize plant growth promoting rhizobacteria (PGPR) for growth enhancement and stress tolerant traits and their efficacy on early establishment of rice seedling. In vitro growth promoting traits revealed that out of 30 PGPR isolates, 18 fixed nitrogen, 17 solubilized tri-calcium phosphate, 29 and 17 produced IAA with or without addition of L-tryptophane. In case of stress tolerant activities, PGPR isolates tolerated pH ranging from 5 to 10, Nacl from 1 to 6% and polyethylene glycol (PEG) from 10 to 40%, respectively. They showed antagonistic activity against Pyricularia oryzae with PIRG values ranging from 7−68%. After two-stage of screening, isolates UPMR7 and UPMR17 were identified based on 16s rRNA gene sequences and matched to the genus Bacillus and Citrobacter with 97−98% similarity. UPMR 7 and UPMR 17 were further evaluated on early growth promotion of rice variety MR219. Results revealed that PGPR inoculation had significant effects on plant growth compared to non-inoculated plants. Thus, it could be suggested that the isolates UPMR7 and UPMR17 have the potential to be used as biofertilizer and bioenhancer in sustainable rice cultivation.

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Isolation and screening of potential actinobacteria for rapid composting of rice straw

Cited by 70 publications

References 33 publications

Discovery of Microorganisms and Enzymes Involved in High-Solids Decomposition of Rice Straw Using Metagenomic Analyses

Discovery of Microorganisms and Enzymes Involved in High-Solids Decomposition of Rice Straw Using Metagenomic Analyses

Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study

Molecular Characterization of Stress Tolerant Plant Growth Promoting Rhizobacteria (PGPR) for Growth Enhancement of Rice

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