Isolation and sequence determination of human brain natriuretic peptide in human atrium

Kambayashi, Yoshikazu; Nakao, Kazuwa; Mukoyama, Masashi; Saito, Yoshihiko; Ogawa, Yoshihiro; Shiono, Satoshi; Inouye, Ken; Yoshida, Nobuo; Imura, Hiroo

doi:10.1016/0014-5793(90)80043-i

Cited by 218 publications

(85 citation statements)

References 20 publications

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“…21 More recently, we have isolated and sequenced human BNP with 32 amino acids in the human heart. 22 These results indicate that the distribution and the processing of BNP show a pronounced species difference. It is likely, therefore, that the physiological and pathophysiological significance of the dual mechanism of ANP and BNP, especially BNP, must await further investigation based on these findings.…”

Section: Discussionmentioning

confidence: 86%

Rat brain natriuretic peptide. Isolation from rat heart and tissue distribution.

et al. 1990

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We have isolated a cardiac natriuretic peptide of 5,000 d from atrial tissues from 500 rats and determined its amino acid sequence. The 5,000 d atrial natriuretic factor was elucidated to be a 45 amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptideand its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence (51-95) of the rat brain natriuretic peptide precursor deduced from the complementary DNA (cDNA) sequence. The cardiac natriuretic peptide with a molecular weight of 5,000, or rat brain natriuretic peptide, was identified as the major storage form and as the sole secretory form derived from the brain natriuretic peptide precursor in the rat heart The rat brain natriuretic peptide level in the atrium was 3.68±0.61 /ig/g, which represents about 4% of that of atrial natriuretic factor. Rat brain natriuretic peptide was also detected in the ventricle. The ratio of brain natriuretic peptide to atrial natriuretic peptide in the ventricle was approximately 30% and much higher than that in the atrium. Rat brain natriuretic peptide, however, was not detectable in the brain. We conclude that the 5,000 d cardiac natriuretic peptide is rat brain natriuretic peptide with 45 amino acids derived from the brain natriuretic peptide precursor and is secreted from the rat heart as a novel cardiac hormone. (Hypertension 1990;15:774-778) B y using radioimmunoassay (RIA) for the ring structure of isoatriaJ natriuretic peptide (iso-ANP), which is the highly conserved sequence of natriuretic peptides, and radioreceptor assay for atrial natriuretic peptides (ANP), we have already demonstrated the presence of a considerable amount of a novel cardiac natriuretic peptide with a molecular weight of 5,000 in the rat atrium, which is distinct from iso-ANP and rat atrial natriuretic factor-(99-126) [rat ANP-(99-126)] isolated previously.

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Section: Discussionmentioning

confidence: 86%

Rat brain natriuretic peptide. Isolation from rat heart and tissue distribution.

et al. 1990

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“…The processing of proBNP forms an N-terminal proBNP (NT-proBNP) fragment with amino acid residues 1-76 and a C-terminal region active BNP with amino acid residues 77-108 (16 ). Because of proteolysis of proBNP, BNP and NT-proBNP are produced in a stoichiometric ratio of 1:1.…”

Section: Bnpmentioning

confidence: 99%

“…Of interest, in that study, glycosylation had no impact. In addition, assays specific to the amino terminus (amino acids [11][12][13][14][15][16][17] are less susceptible to BNP cleavage by neprilysin than epitopes specific for amino acids 14 -21. Currently it is not clear how BNP and NT-proBNP concentrations are affected by treatment with LCZ696 in different conditions.…”

Section: Analytics Of Natriuretic Peptide Assaysmentioning

confidence: 99%

Natriuretic Peptides and Analytical Barriers

Vasile

Jaffe

2017

Clinical Chemistry

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BACKGROUND:The natriuretic peptide system is an endocrine, autocrine and paracrine system that plays an important role in the maintenance of cardiovascular homeostasis. Biomarkers based on these peptides are important diagnostic and prognostic tools for myocardial function.CONTENT: Although natriuretic peptides were discovered more than 2 decades ago, their intricate and complex biology is associated with important questions not yet elucidated. The diversity of circulating forms of natriuretic peptides, the distinct expression of these forms in particular patients, and the heterogeneity of heart failure forms, along with specific assay-related and preanalytic issues, cause assays to be poorly harmonized.

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“…Brain natriuretic peptide (BNP) was first identified in porcine brain by Sudoh et al 6 in 1988, and later isolated by Kambayashi et al 7 from the human atrium in 1990. It belongs to the group of natriuretic peptides.…”

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confidence: 99%

Ventricular natriuretic peptide (BNP) in heart transplantation: BNP correlation with endomyocardial biopsy, laboratory and hemodynamic measures

et al. 2003

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A prospective study of 81 heart transplant (HT) patients was carried out in order to evaluate the evolution of brain natriuretic peptide (BNP) levels in HT patients and compare them with the degree of rejection as determined by endomyocardial biopsy. All patients were subjected to endomyocardial biopsy (532), and determination of BNP and creatinine levels as well as hemodynamic parameters. A control group of 36 volunteers was included. BNP values were significantly greater in HT patients than in healthy volunteers. In the first 3 months, BNP levels in patients with treatable rejection were significantly greater than in patients without graft rejection, although evident overlapping was observed in both distributions and discriminatory potential was low. After the third month, BNP values were similar in patients with and without rejection. Creatinine levels were observed to increase over time after transplantation, but no correlation was observed between the creatinine and BNP levels. A significant positive correlation was observed between BNP and right ventricle and pulmonary arterial pressures.

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Isolation and sequence determination of human brain natriuretic peptide in human atrium

Cited by 218 publications

References 20 publications

Rat brain natriuretic peptide. Isolation from rat heart and tissue distribution.

Rat brain natriuretic peptide. Isolation from rat heart and tissue distribution.

Natriuretic Peptides and Analytical Barriers

Ventricular natriuretic peptide (BNP) in heart transplantation: BNP correlation with endomyocardial biopsy, laboratory and hemodynamic measures

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