Isolation and sequence of a partial vitellogenin cDNA from the cockroach,Blattella germanica (L.) (Dictyoptera, Blattellidae), and characterization of the vitellogenin gene expression

Piulachs, María‐Dolors; Comas, Daniel; Bellés, Xavier

doi:10.1002/(sici)1520-6327(1998)38:3<137::aid-arch4>3.0.co;2-p

Cited by 30 publications

(18 citation statements)

References 23 publications

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“…In control females, vitellogenin production starts on day 1 of adult life (Martín et al, 1998), just when JH production shows rates well above those of previous stages, practically doubling the maximal rates observed in penultimate larval instar (Fig. 1B).…”

Section: Discussionmentioning

confidence: 99%

“…Experiments in vivo (Comas et al, 1999) and in vitro (Comas et al, 2001) have revealed that JH induces vitellogenin gene transcription. In addition, the first signs of vitellogenin mRNA in a normal reproductive cycle appear on day 1 of adult life (Martín et al, 1998), just when JH production begins to reach rates far above those of previous stages (Fig. 1B).…”

Section: Juvenile Hormone Production During the Last Two Larval Instamentioning

confidence: 99%

“…Kunkel (1981) was able to induce vitellogenin production (measured in terms of protein by immunoelectrophoresis) in larvae of Blattella germanica treated with JH III, and concluded that the fat body of the German cockroach could be a useful model to examine the development of a JH response. We have cloned the vitellogenin cDNA in B. germanica (Martín et al, 1998;Comas et al, 2000), which has allowed us to reexplore the subject by measuring vitellogenin expression in terms of mRNA. With these tools, we have reassessed whether the production of vitellogenin exclusively in the adult derives from a metamorphic event involving a change in the adult fat body to perform a new function or a change in the adult corpora allata allowing the production of the high amounts of JH necessary to induce the expression of the vitellogenin gene.…”

Section: Introductionmentioning

confidence: 99%

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Quantity does matter. Juvenile hormone and the onset of vitellogenesis in the German cockroach

Cruz

Martin

Pascual

et al. 2003

Insect Biochemistry and Molecular Biology

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Section: Discussionmentioning

confidence: 99%

Section: Juvenile Hormone Production During the Last Two Larval Instamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantity does matter. Juvenile hormone and the onset of vitellogenesis in the German cockroach

Cruz

Martin

Pascual

et al. 2003

Insect Biochemistry and Molecular Biology

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“…In most of those studies, Vg synthesis was measured by immunological methods and pulse-chase techniques, and was used as an index for hormonal activity. Recently, a Vg cDNA clone for the German cockroach, Blattela germanica, was obtained and utilized for monitoring Vg gene expression (Martin et al, 1998;Comas et al, 1999). The sensitivity was quite high compared to the conventional methods and the analyses at molecular level provided a reliable means to analyze the hormonal regulation on gene expression (Martin et al, 1998;Comas et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Cloning of vitellogenin cDNA of the American cockroach,Periplaneta americana (Dictyoptera), and its structural and expression analyses

Tufail

Lee

Hatakeyama

et al. 2000

Arch. Insect Biochem. Physiol.

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A cDNA expression library constructed from poly (A)(+) RNA prepared from vitellogenic female fat body cells of the American cockroach, Periplaneta americana (Dictyoptera) was screened using a polyclonal antiserum against the 100-kD polypeptide(s) from the egg extract. A partial Vg cDNA clone was obtained and sequenced. The 5' end portion of the cDNA was then obtained by the RACE method, cloned, and sequenced. The combined complete Vg cDNA was 5,854 bp long and contained a single ORF encoding 1,896 amino acids. The entire deduced amino acid sequence was aligned confidently with those of the known insect Vgs. A GL/ICG motif, a number of cysteines at conserved locations following this motif, and a DGXR motif upstream of the GL/ICG motif were present near the C-terminal. The chemically determined N-terminal amino acid sequence of the 170-kD polypeptide from the egg extract completely matched the deduced sequence starting from just after one of the consensus (RXXR) cleavage sites, indicating the occurrence of post-translational cleavage in the fat body cells. The Vg gene begins to be expressed in the 2-day-old adult female fat body cells but is never expressed in ovaries or in male fat body cells. Hemolymph Vg was first detected by immunoblotting in 4-day-old adult females, 2 days after the beginning of gene expression. Western blot analysis of major yolk polypeptides in nine cockroach species belonging to the two superfamilies, Blattoidea and Blaberoidea, using the antisera against P. americana major yolk polypeptides showed that the similarities in Vn antigenicity are basically limited to within a superfamily.

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“…Invertebrate Vtgs lack the PV domain, although some possess similar intervening serine repeats. 48 These insect polyserines are much shorter and share no homology with vertebrate PV. 49 Typically, PV consists of long chains of serine residues interrupted by short stretches of basic amino acids.…”

Section: Vitellogenin Proteins -Interspecific Diversity In Molecular mentioning

confidence: 84%

Vitellogenesis and Vitellogenin Uptake into Oocytes

Ding¹

2005

Hormones and Their Receptors in Fish Reproduction

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The yolk proteins found in the oocyte of oviparous animals are synthesized by the liver as a large lipophosphoglycoprotein precursor, vitellogenin (Vtg), which represents a vital, sole source of nutrient for the developing larvae. Vitellogenesis underlies early development of oviparous animals. The Vtg gene is extremely sensitive to estrogen and other steroid hormones. Thus, vitellogenesis is a useful model for studying hormonal regulation of eukaryotic gene expression. The Oreochromis aureus vitellogenin, OaVtg gene spans 9 kb and contains 34 exons. Although the OaVtg promoter has a nonconsensus TATA, this promoter is capable of driving basal transcription. Two imperfect estrogen response elements, ERE p (proximal) and ERE d (distal), located in the promoter at Ϫ532 and Ϫ1352 elicit a significant increase in estrogen-dependent reporter gene activity. Competition gel mobility shift assays have demonstrated that ERE p and a novel ERE exon2 (located within exon 2) exhibit specific binding of estrogen receptor. Thus, nonconsensus OaVtg EREs contribute to the estrogen-dependent regulation of the OaVtg gene in vivo. During estrogen-induced vitellogenesis, the massive rate of transcription and translation of Vtg followed by its secretion into the blood makes this a major circulating protein that is taken up by membrane-bound vitellogenin receptors (VtgR) found on oocytes. The VtgR, which belongs to the low density lipoprotein receptor family, interacts with the N-terminal region of Vtg Hormones and Their Receptors in Fish Reproduction Downloaded from www.worldscientific.com by UNIVERSITY OF BIRMINGHAM LIBRARY -INFORMATION SERVICES on 03/20/15. For personal use only. Vitellogenesis and Vitellogenin Receptor 255via electrostatic attraction. Once in the oocytes, Vtg is cleaved into yolk proteins (lipovitellin and phosvitin), which are stored as nutrients for the developing embryo.

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Isolation and sequence of a partial vitellogenin cDNA from the cockroach,Blattella germanica (L.) (Dictyoptera, Blattellidae), and characterization of the vitellogenin gene expression

Cited by 30 publications

References 23 publications

Quantity does matter. Juvenile hormone and the onset of vitellogenesis in the German cockroach

Quantity does matter. Juvenile hormone and the onset of vitellogenesis in the German cockroach

Cloning of vitellogenin cDNA of the American cockroach,Periplaneta americana (Dictyoptera), and its structural and expression analyses

Vitellogenesis and Vitellogenin Uptake into Oocytes

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