Isolation and sequence of the <i>MIG1</i> homologue from the yeast <i>Candida utilis</i>

Delfín, Julieta; Perdomo, Walmer; Garcı́a, Bianca; Menéndez, Javier

doi:10.1002/yea.705

Cited by 6 publications

(4 citation statements)

References 22 publications

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“…Finally, the entire sequence of SoMig1p was compared with those from complete Mig1p and CreA sequences of different organisms (Table 1). C. utilis Mig1p (Delfin et al ., 2001) is the most similar to SoMig1p, with 24% identity. This similarity is consistent with the short phylogenetic distance existing between these two organisms (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

Molecular and functional analysis of a MIG1 homologue from the yeast Schwanniomyces occidentalis

Carmona

Barrado

Jiménez

et al. 2002

Yeast

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A putative glucose repressor MIG1-homologue (SoMIG1) was isolated from the amylolytic yeast Schwanniomyces occidentalis. Degenerate primers were designed from the conserved zinc finger regions of Mig1 and CreA proteins from different organisms. PCR using these primers and S. occidentalis genomic DNA as template yielded a single 128 bp product. This fragment was used as a DNA probe to screen a S. occidentalis genomic library. Analysis of the positive clones led to the isolation by PCR of a DNA fragment, which contained an open reading frame (ORF) that would encode a 458 amino acid polypeptide. The DNA binding and effector domains of this putative protein showed an identity of 71% and 15%, respectively, to those of the Mig1 protein from Saccharomyces cerevisiae. The SoMIG1 gene complemented a mig1 mutant of this yeast, which suggests that in S. occidentalis SoMIG1 is a glucose repressor.

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Section: Resultsmentioning

confidence: 99%

Molecular and functional analysis of a MIG1 homologue from the yeast Schwanniomyces occidentalis

Carmona

Barrado

Jiménez

et al. 2002

Yeast

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“…According to the literature, transcriptional regulators of maltase genes from other yeasts can function in S. cerevisiae . For example, the Mig1 repressor homologues from Candida utilis [27], K. lactis [28] and Schwanniomyces occidentalis [29] complement the Mig1 deficiency in S. cerevisiae , and the MAL activator of C. albicans (CASUC1) can substitute the function of MAL63 activator protein in S. cerevisiae [30]. We inspected the GenBank data resulting from the partial genomic sequencing project of H. polymorpha [31] in order to find nucleotide sequences that might encode homologues of S. cerevisiae MAL activator and Mig1 repressor proteins.…”

Section: Resultsmentioning

confidence: 99%

Section: Regulation Of the S Cerevisiae Maltase Gene Mal62 In Hp201hmentioning

confidence: 99%

Regulation of the maltase gene promoter in and

Alamäe

Pärn

Viigand

et al. 2003

FEMS Yeast Research

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Hansenula polymorpha is an exception among methylotrophic yeasts because it can grow on the disaccharides maltose and sucrose. We disrupted the maltase gene (HPMAL1) in H. polymorpha 201 using homologous recombination. Resulting disruptants HP201HPMAL1Delta failed to grow on maltose and sucrose, showing that maltase is essential for the growth of H. polymorpha on both disaccharides. Expression of HPMAL1 in HP201HPMAL1Delta from the truncated variants of the promoter enabled us to define the 5'-upstream region as sufficient for the induction of maltase by disaccharides and its repression by glucose. Expression of the Saccharomyces cerevisiae maltase gene MAL62 was induced by maltose and sucrose, and repressed by glucose if expressed in HP201HPMAL1Delta from its own promoter. Similarly, the HPMAL1 promoter was recognized and correctly regulated by the carbon source in a S. cerevisiae maltase-negative mutant 100-1B. Therefore we suggest that the transcriptional regulators of S. cerevisiae MAL genes (MAL activator and Mig1 repressor) can affect the expression of the H. polymorpha maltase gene, and that homologues of these proteins may exist in H. polymorpha. Using the HPMAL1 gene as a reporter in a H. polymorpha maltase disruption mutant it was shown that the strength of the HPMAL1 promoter if induced by sucrose is quite comparable to the strength of the H. polymorpha alcohol oxidase promoter under conditions of methanol induction, revealing the biotechnological potential of the HPMAL1 promoter.

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“…Repression of MLS1 occurs through a Mig1 site ( Caspary et al 1997 ). It has been shown that a MIG1 gene deletion in Saccharomyces cerevisiae can be rescued by MIG1 from Candida utilis ( Delfin et al 2001 ) and K. lactis ( Cassart et al 1995 ), indicating that MIG1 has conserved its general function as well.…”

Section: Introductionmentioning

confidence: 99%

DivergentMLS1Promoters Lie on a Fitness Plateau for Gene Expression

2016

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Qualitative patterns of gene activation and repression are often conserved despite an abundance of quantitative variation in expression levels within and between species. A major challenge to interpreting patterns of expression divergence is knowing which changes in gene expression affect fitness. To characterize the fitness effects of gene expression divergence, we placed orthologous promoters from eight yeast species upstream of malate synthase (MLS1) in Saccharomyces cerevisiae. As expected, we found these promoters varied in their expression level under activated and repressed conditions as well as in their dynamic response following loss of glucose repression. Despite these differences, only a single promoter driving near basal levels of expression caused a detectable loss of fitness. We conclude that the MLS1 promoter lies on a fitness plateau whereby even large changes in gene expression can be tolerated without a substantial loss of fitness.

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Isolation and sequence of the MIG1 homologue from the yeast Candida utilis

Cited by 6 publications

References 22 publications

Molecular and functional analysis of a MIG1 homologue from the yeast Schwanniomyces occidentalis

Molecular and functional analysis of a MIG1 homologue from the yeast Schwanniomyces occidentalis

Regulation of the maltase gene promoter in and

DivergentMLS1Promoters Lie on a Fitness Plateau for Gene Expression

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