Isolation and Serological Characterization of Porcine Reproductive and Respiratory Syndrome (PRRS) Viruses from Pigs with Reproductive and Respiratory Disorders in Japan.

Murakami, Yosuke; Kato, Azusa; Tsuda, Toshitaka; Morozumi, Tatsuya; Miura, Yasuo; Sugimura, Takaaki

doi:10.1292/jvms.56.891

Cited by 57 publications

(52 citation statements)

References 9 publications

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“…The indirect immunofluorescence assay (IIFA) test of PRRS virus [6] and growth agglutination (GA) test of E. rhusiopathiae [8] were carried out by methods described previously.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Attenuated Erysipelothrix rhusiopathiae Vaccine in Pigs Infected with Porcine Reproductive Respiratory Syndrome Virus.

Sakano

Shibata

Namimatsu

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. Twenty 2nd specific pathogen-free pigs were divided into 4 groups: Group A were infected with porcine reproductive and respiratory syndrome (PRRS) virus at 6 weeks of age and treated with available swine erysipelas and swine fever combined vaccine (vaccinated) at 7 weeks of age; Group B were vaccinated at 7 weeks of age and infected with PRRS virus at 8 weeks of age; Group C were vaccinated at 7 weeks of age: Group D were neither vaccinated nor infected with PRRS virus. All pigs were challenged to Erysipelothrix rhusiopathiae C42 strain at 10 weeks of age. No clinical signs appeared after vaccination of group A and B pigs, thus confirming that the safety of the vaccine was not influenced by infection with PRRS virus. None of the pigs in Groups A and C developed erysipelas after challenge exposure to E. rhusiopathiae. In contrast, fever and/or urticaria appeared transiently in all pigs of Group B after challenge exposure. At the time of challenge exposure to E. rhusiopathiae, the PRRS virus titer was high in sera of Group B, but was low in those from Group A. However, vaccination of pigs with attenuated E. rhusiopathiae was effective in dual infection with PRRS virus and E. rhusiopathiae, because the clinical signs were milder and the E. rhusiopathiae strain was less recovered from these pigs compared to pigs of group D. -KEY WORDS: Erysipelothrix rhusiopathiae, experimental infection, PRRS, swine, vaccine.

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“…The indirect immunofluorescence assay (IIFA) test of PRRS virus [6] and growth agglutination (GA) test of E. rhusiopathiae [8] were carried out by methods described previously.…”

Section: Methodsmentioning

confidence: 99%

“…Reproductive disturbance in sows and respiratory distress in growing pigs were encountered around 1987 due to the outbreaks of porcine reproductive and respiratory syndrome (PRRS), and PRRS virus was isolated in 1993 [6,12]. It has been believed that PRRS was associated with other microbial infections in growing pigs [10,12].…”

mentioning

confidence: 99%

Effect of Attenuated Erysipelothrix rhusiopathiae Vaccine in Pigs Infected with Porcine Reproductive Respiratory Syndrome Virus.

Sakano

Shibata

Namimatsu

et al. 1997

The Journal of Veterinary Medical Science

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“…Isolation of PRRS virus (PRRSV), the causal agent of the disease, was first reported in Europe in 1991 and later in North America in 1992 . Since then, the virus has been identified in most of pig-producing regions worldwide and has caused significant economic losses in swine industries (Dea et al, 1992;Paton et al, 1992;Murakami et al, 1994). PRRSV is a member of the family Arteriviridae in the order Nidovirales (Cavanagh, 1997) and an enveloped virion containing a single-stranded positive-sense RNA genome.…”

Section: Introductionmentioning

confidence: 99%

Different immunological features of two genetically distinct type 2 porcine reproductive and respiratory syndrome (PRRS) viruses

Shabir¹,

Khatun²,

Kim³

2014

Korean Journal of Veterinary Service

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Although it has been generally accepted that porcine reproductive and respiratory syndrome virus (PRRSV) induces weak and delayed protective immunity after infection, it is unclear that the same immunological features can be applicable to all PRRS viruses because huge genetic variation exists even among the same genotypes of PRRSV (Type 1 and 2). In the current study, two genetically distinct type 2 PRRSV strains (VR-2332 and JA142) which showed approximately 90% nucleotide homology based on ORF5 sequences were characterized by both in vitro and in vivo assessments to determine the immunological features of the viruses. For in vitro assessment, porcine alveolar macrophages (PAM) were infected with the viruses at 10 −3 multiplicity of infection (MOI) and then supernatants and cells were collected separately at 36 hrs post infection to determine the relative expression levels of IL-1α, IL-12, TNF-α and INF-α/β by quantitative RT-PCR. In addition, five PRRSV-free pigs were inoculated with either of JA142 or VR2332 for in vivo assessment. Serum samples were collected every week until 6 weeks post challenge. The serum samples were analyzed for the levels of viremia, PRRSV nucleocapsid-specific antibody and virus neutralizing antibody. Based on those assessments, the two viruses showed different patterns of cytokine expression in PAM and immune responses in pigs after infection. These results indicate that genetically distinct PRRSV strains have different immunological features, which might be criteria for virus classification and selection of candidate virus strains for vaccine development in the future.

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“…This has caused serious economic loss to pig farming industries due to the decreased growth rate of weaning piglets and the reproduction abnormalities [18,19]. In Japan, PRRS has been reported since 1993 [15,20] and the reported data show a high prevalence rate [2]. In many commercial farms in the world, some endemic respiratory diseases cause a mixed infection called porcine respiratory disease complex (PRDC), and PRRS is one of the most basic parts of PRDC [4,21].…”

mentioning

confidence: 99%

Evaluation of Enzyme-Linked Immunosorbent Assay (ELISA)and Immunofluorescent Antibody (IFA) Test for the Detection of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Antibody in Pigs from Conventional Farms.

Yahara¹,

Ohkubo²,

Kariwa

et al. 2002

J. Vet. Med. Sci.

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ABSTRACT. To evaluate the immunofluorescent antibody (IFA) test and enzyme-linked immunosorbent assay (ELISA) for detecting the porcine reproductive and respiratory syndrome virus (PRRSV) antibody, conventional pigs in PRRSV-positive and -negative commerci al farms were examined. Antibody development patterns in ELISA and IFA tests were compared in 3 week old piglets experimentally infected with the PRRSV. The virus was detected from 2 days post infection (PI) and then the antibody titers and S/P ratios rose by both methods. A total of 208 serum samples were collected from 4 PRRSV-negative farms and 210 samples from PRRSV-positive farms, and were tested for the PRRSV antibody by IFA and ELISA. The titer of 64 should be set as the cut-off point in IFA for field sera. Similarly, the cut-off S/P ratio should be set at 0.4 in ELISA. A high degree of correlation was observed between antibody titers by the two methods in these 418 samples, with a correlation coefficient of 0.84. The coincidence rate between the two tests was 84.7% (354/418). In non-coincident cases, ELISA was able to detect the antibody with a low titer in the serum samples which were negative in IFA but from PRRSV positive farms. ELISA was more sensitive than IFA to detect PRRSV infected animals or farms.

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Isolation and Serological Characterization of Porcine Reproductive and Respiratory Syndrome (PRRS) Viruses from Pigs with Reproductive and Respiratory Disorders in Japan.

Cited by 57 publications

References 9 publications

Effect of Attenuated Erysipelothrix rhusiopathiae Vaccine in Pigs Infected with Porcine Reproductive Respiratory Syndrome Virus.

Effect of Attenuated Erysipelothrix rhusiopathiae Vaccine in Pigs Infected with Porcine Reproductive Respiratory Syndrome Virus.

Different immunological features of two genetically distinct type 2 porcine reproductive and respiratory syndrome (PRRS) viruses

Evaluation of Enzyme-Linked Immunosorbent Assay (ELISA)and Immunofluorescent Antibody (IFA) Test for the Detection of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Antibody in Pigs from Conventional Farms.

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