Isolation and Serological Survey of Salmonella in Pigs in Japan.

Abstract

ABSTRACT. A total of 267 fecal and serum samples collected from individual pigs reared on a Salmonella-positive farm were subjected to bacteriological and serological examinations of Salmonella. Salmonella was isolated from 47 pigs (17.6%) and prevalence of antibody to lipopolysaccharide (LPS) of S. Typhimurium, which was partly common to S. O4, 12: d: -, was observed in 90 pigs (33.7%). Salmonella was isolated from 26 (28.9%) of 90 antibody-positive pigs and 21 (11.9%) of 177 antibody-negative pigs. Twenty-one of 36 pigs (58.3%) positive for S. O4, 12: d: -, five of 10 pigs (50.0%) positive for S. Havana, and none for S. Anatum had antibodies. Thus, seropositive rates were higher than isolation-positive rates, and antibody prevalence was associated with serovars of the isolates. Then, we analyzed antibody prevalence among pigs on Japanese pig farms. The antibodies to LPS of S. Typhimurium were found in 195 of 1,498 pigs (13.0%) and in at least one serum sample on 35 of 52 farms (67.3%). Our results indicate that Salmonella does not seem to be so prevalent in pigs though it is widely prevalent among pig farms.

Salmonella entericaSubclinical Infection: Bacteriological, Serological, Pulsed-Field Gel Electrophoresis, and Antimicrobial Resistance Profiles—Longitudinal Study in a Three-Site Farrow-to-Finish Farm

“…are one of the most important foodborne pathogens transmitted to humans worldwide (Asai et al, 2002;Beloeil et al, 2003;Oliveira et al, 2005). Pork is the second most important source of Salmonella spp.…”

“…prevalence in pig herds, bacteriological examinations are performed on fecal samples or tissues from clinically affected animals (Asai et al, 2002). Culturing fecal samples for Salmonella spp.…”

Detection of Salmonella spp. Isolates from Specimens due to Pork Production Chains in Hue City, Vietnam

“…Salmonella spp. are known to be the causative agents of salmonellosis in humans and animals [1,3,4], and most can cause gastroenteritis when ingested by eating contaminated foods. It is generally considered that the risk due to this pathogen is growing because of the lack of good hygienic slaughtering practices and implementation of cold chains.…”

Enrichment for Isolating Salmonella Choleraesuis and other Salmonella spp. from Pigs

“…Many Salmonella serovars other than S. Choleraesuis cause food poisoning in human. Previous studies have shown that several serovars of Salmonella are prevalent among pigs [2,4,6,[11][12][13]. Hence, Salmonella prevalence among pigs causes a significant health problem for pigs and for people.…”

“…In several recent studies of experimental infection of pigs with S. Choleraesuis, Rappaport Vassiliadis broth (RV) [19] was used as the enrichment broth [1,10,16]. Besides, Hajnatetrathionate broth (HTT) has widely been used as an enrichment broth for Salmonella in Japan [2,4,13,14]. In this report, we assess the enrichment for isolation of S. Choleraesuis and Salmonella serovars other than S. Choleraesuis from pigs.…”