Isolation and serological survey of chlamydiosis in feral pigeons and crows in Hokkaido.

Chiba, Nobuyuki; Arikawa, Jiro; Takashima, Ikuo; Hashimoto, Nobuo

doi:10.1292/jvms1939.46.243

Cited by 19 publications

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“…The pre valence of chlamydial infection in the flocks va r i e d markedly ( Table 2). Pigeon lofts in this study contained a significant number of birds shedding C psittaci (10% to 57% per flock), in agreement with overseas surveys of pigeon flocks, 31,32 in which prevalences as high as 60% were commonly recorded. Psittacine birds in this study also provided a large proportion of positive results with pet shops and private aviaries having prevalences of 5% to 42%.…”

Section: Bacteria Tested Included Salmonella Ty P H I M U R I U M Esupporting

confidence: 89%

Methods of detection of Chlamydia psittaci in domesticated and wild birds

McElnea¹,

Cross

1999

Aust Veterinary J

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Section: Bacteria Tested Included Salmonella Ty P H I M U R I U M Esupporting

confidence: 89%

Methods of detection of Chlamydia psittaci in domesticated and wild birds

McElnea¹,

Cross

1999

Aust Veterinary J

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“…C. psittaci strain P-1041, which was isolated from a feral pigeon (7), was used in this study. The organisms were propagated in HeLa 229 cells and stored at -80 C until use.…”

Section: Methodsmentioning

confidence: 99%

Neutralization of Chlamydia psittaci with Monoclonal Antibodies

Ando

Takashima

Hashimoto

1993

Microbiology and Immunology

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Neutralization of Chlamydia (C.) psittaci avian strain P-1041 was examined in vitro using monoclonal antibodies (MAbs). Of the 10 MAbs used, 6 were found to exhibit neutralizing capability. These include 3 against major outer membrane protein (MOMP), 1 against lipopolysaccharide (LPS) and 2 against other protein molecules [90 kilodalton (kDa) and 90/50 kDa,]. Most neutralizing MAbs were dependent on complement for efficient neutralization, while a strainspecific MAb (2B5) against the 90 kDa protein displayed a different requirement for complement and neutralized the infectivity of the P-1041 at high concentrations without complement. By competitive inhibition enzyme-linked immunosorbent assay (competitive inhibition ELISA), all 3 neutralizing anti-MOMP MAbs were demonstrated to recognize different epitopes found in very close proximity to each other on the outer membrane.

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“…and Cryptococcus. In Japan, S. Typhimurium [5,14,15], C. psittaci [3,6,8] and Mycobacterium spp. [10,11] have been isolated from feral pigeons and the frequency of Salmonella spp.…”

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Bacteriological Survey of Feces from Feral Pigenons in Japan

Tanaka

Miyazawa

Watarai

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. Some public areas in Japan such as parks and gardens can be highly contaminated with pigeon feces. We examined levels of four bacterial contaminations in fecal samples from feral pigeons in 7 prefectures. We isolated Salmonella Typhimurium and S. Cerro from 17 (3.9%) of 436 samples, as well as Mycobacterium spp. including M. avium-intracellulare complex from 29 (19.0%) of 153 samples. The polymerase chain reaction detected Chlamydia psittaci and C. pecorum in 106 (22.9%) of 463 samples, but E. coli O-157 was not isolated from any of the samples. Our results indicate that pigeon feces are a source of several zoonotic agents for birds, animals and humans. KEY WORDS: feces, pigeon, salmonella.J. Vet. Med. Sci. 67(9): 951-953, 2005 Pigeons (Columba livia) are widely distributed in urban and rural areas of Japan, and come into close contact with humans in parks, temples, shrines, public gardens and railroad stations. A recent increase in the number of pigeons has raised public health concerns [7]. Pigeons are potential reservoirs for several pathogenic microorganisms, including Chlamydia spp., Salmonella spp. and Cryptococcus. In Japan, S. Typhimurium [5,14,15], C. psittaci [3,6,8] and Mycobacterium spp. [10,11] have been isolated from feral pigeons and the frequency of Salmonella spp. and Chlamydia spp. is particularly high [1,2,12]. The presence of pigeon feces in public parks and railroad stations has contributed to the spread of infectious agents in the environment. We therefore extensively surveyed four pathogens (Salmonella spp., Escherichia coli 0-157, Mycobacterium spp. and Chlamydia spp.) in pigeon feces collected from public areas in Japan from 2003 to 2004.Fecal samples (fresh or dry composite) were collected from 38 public areas in 7 prefectures (Hokkaido, Tokyo, Kanagawa, Gifu, Aichi, Osaka and Hiroshima) in Japan. Dry samples were placed in polyethylene bags, and fresh samples were placed in 15-ml tubes containing 1 ml of phosphate-buffered saline.To isolate Salmonella spp., about 0.5 to 3 g of fecal samples were cultured in Hajna tetrathionate broth (Eiken, Tokyo, Japan) and then plated on DHL agar (Eiken) and on CHROMagar Salmonella (CHROMagar Microbiology, Paris, France). Salmonella-like isolates on DHL or CHROMagar plates were examined using TSI (Eiken) and LIM (Eiken) media and confirmed by slide and tube agglutination with commercial antisera (Denka Seiken, Tokyo, Japan). To isolate E. coli O-157, about 2 to 3 g of feces was cultured in mEC broth (Kyokutou, Tokyo, Japan) with novobiocin, and examined using a PATH-STIK KIT (Celsis Lumac, B. V. Landgraaf, The Netherlands). We finally confirmed the presence of E. coli O-157 by slide agglutination with a specific antibody (Denka Seiken). To isolate Mycobacterium spp. about 1 to 3 g of fecal samples were mixed with 0.1% acriflavine and 4% NaOH, and inoculated on Ogawa slant medium (Nissui, Tokyo, Japan). Colonies on the slants were visualized by Ziehl-Neelsen staining, and Mycobacterium spp. were identified using the DNA-DNA hybridization ...

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Isolation and serological survey of chlamydiosis in feral pigeons and crows in Hokkaido.

Cited by 19 publications

References 0 publications

Methods of detection of Chlamydia psittaci in domesticated and wild birds

Methods of detection of Chlamydia psittaci in domesticated and wild birds

Neutralization of Chlamydia psittaci with Monoclonal Antibodies

Bacteriological Survey of Feces from Feral Pigenons in Japan

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