Isolation and some properties of the nucleoprotein complex <i>of Propionibacterium freudenreichii </i>subsp.<i>shermanii</i>

Vorobjeva, Lena I.; Zinchenko, Anatoly; Khodjaev, E.Yu.; Ponomareva, G. M.; Гордеева, Е. А.

doi:10.1051/lait:1999115

Cited by 6 publications

(4 citation statements)

References 2 publications

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“…This reinforces their potentialities as probiotics. The characterisation of a cytoplasmic protein, which exhibits reactivating effects on both prokaryotic and eukaryotic organisms [29,30], might further highlight the beneficial role of these bacteria in human alimentation [27].…”

Section: Introductionmentioning

confidence: 99%

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Jan

Leverrier

Proudy

et al. 2002

Lait

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-Propionibacteria, which are largely used as Swiss-type cheese starters, also constitute promising human probiotics because of their beneficial effects on intestinal bifidobacteria and bacterial enzyme activities. To be efficient, they must survive acid stress imposed within the stomach and the surfactant effects of bile salts. We thus investigated the ability of Propionibacterium freudenreichii (strain Propiofidus ® SI41) to adapt to, and survive these stresses. In an in vivo study, 7 volunteers received Propiofidus ® SI41 in 3 different modes. The probiotic was supplied during 3 periods (i) in classical capsules at a low dose (5 × 10 9 CFU·d -1 ), (ii) in the same capsules at a higher dose (5 × 10 10 CFU·d -1 ), (iii) in acid-resistant capsules at the low dose. In the first case, propionibacteria were recovered above 10 5 CFU·g -1 in only 8 out of 14 faecal samples. In the two following cases, they were enumerated in all faecal samples between 10 5 and 10 7 CFU·g -1 . The recovery of live propionibacteria in faecal samples seemed to be related to a higher content in short chain fatty acids. This study, which clearly showed the role of gastric protection in the survival of propionibacteria, led us to investigate stress adaptation in this strain. Acid tolerance response was evidenced: transient exposure to pH 5 afforded protection towards acid challenge at pH 2. Moreover, we showed that pre-exposure to a moderate bile salts pre-treatment at 0.2 g·L -1 sharply increased its survival against a subsequent lethal challenge (1 g·L -1 ). Important changes in cellular protein synthesis were observed during adaptation using two-dimensional electrophoresis. Global protein synthesis decreased during adaptation treatments. In contrast, specific proteins were overexpressed during acid adaptation and others during bile salts adaptation. Among the up-regulated polypeptides, a carboxyl carrier protein (BCCP) and the recombinase RecR were identified during both stress responses while the universal chaperonins GroEL and GroES, as well as the replicase RepB, were only up-regulated during acid adaptation. These results bring new insights on the tolerance of P. freudenreichii to acid and bile salts, which should be taken into consideration for the development of probiotic preparations. Résumé -Survie et effets bénéfiques des bactéries propioniques dans l'intestin humain : étu-des in vivo et in vitro. Les propionibactéries, qui sont largement utilisées comme levain d'affinage des fromages à pâte pressée cuite, constituent également des probiotiques humains prometteurs du fait de leurs effets bénéfiques sur la flore bifide et les activités enzymatiques bactériennes, au niveau de l'intestin. Pour être efficaces, elles doivent survivre au stress acide subi dans l'estomac et à l'action détergente des sels biliaires. Nous avons donc étudié la capacité de Propionibacterium freudenreichii (souche Propiofidus ® SI41) à s'adapter et à survivre à ces stress. Dans une étude in vivo, 7 volontaires ont reçu Propiofidus ® SI41 selon 3 mo...

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Section: Introductionmentioning

confidence: 99%

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Jan

Leverrier

Proudy

et al. 2002

Lait

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“…Other extracellular proteins that confer a protective effect have been reported for E. coli and P. freudenreichii (Nikolaev 1996; Vorobjeva et al . 1999).…”

Section: Discussionmentioning

confidence: 92%

“…Rowbury (2001) has described the detailed physiological characteristics of a two‐component extracellular regulatory system in Escherichia coli where an extracellular compound produced by growing cells can be converted to an induction compound by stress factors, leading to a tolerance response. Nikolaev (1996) has also described extracellular protectants in E. coli , while Vorobjeva et al . (1999) have isolated an extracellular nucleoprotein from Propionibacterium freudenreichii subsp.…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel stress resistance mechanism in Campylobacter jejuni

2003

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Aim: To study stress resistance mechanisms in Campylobacter spp. Methods and Results: Campylobacter strains were grown to the appropriate phase in Brucella broth. The cells were diluted into either cell-free spent medium (obtained by filtration of a grown culture) or a freshly prepared medium and the pH reduced to 4AE5, a lethal pH value. At suitable time intervals survivors were enumerated on Campylobacter blood free selective agar base. The cell-free spent medium from mid-exponential and stationary phase had a protective effect on acid and thermal stress in Campylobacter jejuni CI 120, a natural isolate. The protective effect of the extracellular compound was not significantly inactivated by boiling, but was inactivated by proteinase. Conclusions: The present study suggests that a protein (or proteins) accumulated by C. jejuni CI 120 during growth may play an active role in the induction of stress responses and that this protein is heat stable. Significance and Impact of the Study: The results indicate that C. jejuni CI 120, a natural isolate, has the ability to use extracellular signalling mechanisms to induce tolerance to stress factors. This is a major advancement in the understanding of the physiological basis for survival of C. jejuni in the environment.

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“…Propionibacteria are also known for their high production of vitamin B 12 and this has led to the development of commercially interesting production processes [72]. Since some Propionibacterium sp.…”

Section: Introductionmentioning

confidence: 99%

Production of tetrapyrrole compounds and vitamin B12 using genetically engineering of Propionibacterium freudenreichii. An overview

Murooka

Piao

Kiatpapan

et al. 2005

Lait

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-Propionibacterium freudenreichii is a commercially important bacterium that is used in the production of cheeses, cobalamin (vitamin B 12 ) and propionic acid. Metabolic engineering using genetically improved strains will make the fermentation process more economical and also enhance the quality of the products. Host-vector systems and expression vectors using strong promoters from P. freudenreichii were developed in propionibacteria. By using these expression vectors and amplification of various genes, productions of 5-aminolevulinic acid, tetrapyrrole compounds and vitamin B 12 were reported. Here, we review the advancement of genetic engineering in P. freudenreichii in recent years, covering the molecular aspects of the formation of tetrapyrrole compounds and vitamin B12.

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Isolation and some properties of the nucleoprotein complex of Propionibacterium freudenreichii subsp.shermanii

Cited by 6 publications

References 2 publications

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Identification of a novel stress resistance mechanism in Campylobacter jejuni

Production of tetrapyrrole compounds and vitamin B12 using genetically engineering of Propionibacterium freudenreichii. An overview

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