Isolation and structural characterization of exopolysaccharide from marine Bacillus sp. and its optimization by Microbioreactor

Wagh, Vasudev S.; Said, Madhukar S.; Bennale, Jagadish S.; Dastager, Syed G.

doi:10.1016/j.carbpol.2022.119241

Cited by 15 publications

(9 citation statements)

References 53 publications

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“…The NMR spectra between the commercial standards and analyzed LASM12 EPS were qualitatively equivalent. The peaks are all attributable to hydrogen signals in a repeating fructose monomer, which are labeled (1 to 6) in Figure 4 [ 48 ]. The minor peaks in the LASM12 EPS sample, which are absent in the standard levan samples, may be attributable to other sugars or sample contaminants, for which further analysis would be needed for fuller characterization.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Levan Fructan Produced by a Gluconobacter japonicus Strain Isolated from a Sugarcane Processing Facility

Bruni,

Qi,

Terrell

et al. 2024

Microorganisms

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During raw sugarcane processing, a significant portion of lost sucrose is attributable to microbial degradation. Sucrose consumption by many bacteria is also linked to the production of exopolysaccharides (EPS) such as dextrans and fructans. These resulting EPS cause operational challenges during raw sugar manufacturing. Here, we report the characterization of EPS from a fructan-forming Gluconobacter japonicus bacterium that we previously isolated from a Louisiana sugarcane factory. The genome sequencing revealed the presence of two encoded levansucrase genes, lsrA and lsrB. One levansucrase, LsrB, was detected in the secreted protein fraction of G. japonicus LASM12 by QTOF LC-MS. The spotting assays indicated that G. japonicus produces EPS using sucrose and raffinose as substrates. The G. japonicus EPS correlated with levan fructan commercial standards by 1H-NMR, and with the characteristic carbohydrate fingerprint region for FTIR spectra, confirming that the G. japonicus EPS is levan fructan. The glycosyl composition and glycosyl linkage analysis revealed a linear β-2,6-fructofuranosyl polysaccharide with occasional (5.7%) β-2,1-fructofuranosyl branches. The gel permeation chromatography of the levan fructan EPS showed two main peaks at 4.5 kDa and 8 kDa and a very minor peak at 500 kDa. G. japonicus was identified as a producer of levan fructan. These findings will be useful for future studies aimed at evaluating the impact of levan fructans on sugar crop processing, which have been historically underestimated in industry.

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Section: Resultsmentioning

confidence: 99%

Characterization of Levan Fructan Produced by a Gluconobacter japonicus Strain Isolated from a Sugarcane Processing Facility

Bruni,

Qi,

Terrell

et al. 2024

Microorganisms

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“…A Box-Wilson central composite design, also known as a central composite design (CCD), is widely used in response surface methods to develop a second-order polynomial for the response variables without employing a full factorial design of tests [ 34 ]. According to the findings of the Plackett–Burman experiment, CCD was used to analyze and optimize the levels of the parameters, as well as to study the interaction effects among the most significant independent variables affecting cyanobacterial polysaccharide synthesis.…”

Section: Methodsmentioning

confidence: 99%

Characterization and optimization of exopolysaccharide extracted from a newly isolated halotolerant cyanobacterium, Acaryochloris Al-Azhar MNE ON864448.1 with antiviral activity

Saad,

Sidkey,

El-Fakharany

2024

Microb Cell Fact

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Several antiviral agents lost their efficacy due to their severe side effects and virus mutations. This study aimed to identify and optimize the conditions for exopolysaccharide (EPS) production from a newly isolated cyanobacterium, Acaryochloris Al-Azhar MNE ON864448.1, besides exploring its antiviral activity. The cyanobacterial EPS was purified through DEAE-52 cellulose column with a final yield of 83.75%. Different analysis instruments were applied for EPS identification, including Fourier-transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), and gas chromatographic-mass spectrometry (GC–MS). Plackett–Burman’s design demonstrated that working volume (X1), EDTA (X2), inoculum size (X3), CaCl2 (X4), and NaCl (X5) are the most important variables influencing EPS production. Central composite design (CCD) exhibited maximum EPS yield (9.27 mg/mL) at a working volume of 300 mL in a 1 L volumetric flask, EDTA 0.002 g/L, inoculum size 7%, CaCl2 0.046 g/L, and NaCl 20 g/L were applied. EPS showed potent antiviral activities at different stages of herpes simplex virus type-1 and 2 (HSV-1, HSV-2), adenovirus (ADV) and coxsackievirus (A16) infections. The highest half-maximal inhibitory concentration (IC50) (6.477 µg/mL) was recorded during HSV-1 internalization mechanism, while the lowest IC50 (0.005669 µg/mL) was recorded during coxsackievirus neutralization mechanism.

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“…In a similar study, Wagh et al reported that Bacillus sp. SGD-03 produced 2.16 g L −1 EPS in nutrient broth containing 2% glucose (Wagh et al, 2022). Furthermore, Bacillus licheniformis isolated from the thermal fluid sample can secrete 165 mg L −1 EPS in MD 162 minimal medium containing 0.6% glucose (Caccamo et al, 2020).…”

Section: Optimization Of Eps Yieldmentioning

confidence: 99%

“…EPS secreted by marine Bacillus sp. exhibited a fibrous network structure with a relatively smooth surface (Wagh et al, 2022). EPS produced by probiotic Bacillus licheniformis AG-06 showed uneven smooth surfaces with a network-like structure (Vinothkanna et al, 2021).…”

Section: Microstructure Of Bpsmentioning

confidence: 99%

The structure, characterization and dual-activity of exopolysaccharide produced by Bacillus enclensis AP-4 from deep-sea sediments

Li²,

Zhang³

et al. 2022

Front. Mar. Sci.

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In recent years, the exopolysaccharide (EPS) produced by deep-sea bacteria has attracted the interest of various researchers. In the present study, we have explored the properties and structure of a novel exopolysaccharide (called BPS) produced by Bacillus enclensis AP-4 from deep-sea sediments. The maximum yield of BPS was 4.23 ± 0.17 g L−1 in a 2216E modified medium. 1H NMR studies of the purified BPS displayed α and β-configuration sugar residues, including mannose, glucosamine, glucose, galactose, and xylose in a molar ratio of 1.00: 0.09: 0.04: 0.09: 0.07. BPS showed a molecular weight of 23,434 Da and was abundant in hydroxyl and amino residues. In addition, BPS exhibited a rod-like structure with a rough surface and was dominated by C, N, and O elements. The exopolysaccharide demonstrated remarkable thermal stability, high degradation temperature, and excellent emulsification capacity compared to most reported exopolysaccharides. Moreover, BPS displayed better quenching activities against the four radicals, which provided favorable protection for the strain. Finally, the freezing experiment investigated the cryoprotective effect of BPS on E. coli and S. aureus. BPS effectively improved the cell survival ratio and maintained the activity of Na+/K+-ATPase, which facilitates culture preservation. To the best of our knowledge, our work is the first report suggesting that marine exopolysaccharide has dual-activity. This work presents the foundation for the analysis of the structure and properties of exopolysaccharides produced by deep-sea bacteria.

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Isolation and structural characterization of exopolysaccharide from marine Bacillus sp. and its optimization by Microbioreactor

Cited by 15 publications

References 53 publications

Characterization of Levan Fructan Produced by a Gluconobacter japonicus Strain Isolated from a Sugarcane Processing Facility

Characterization of Levan Fructan Produced by a Gluconobacter japonicus Strain Isolated from a Sugarcane Processing Facility

Characterization and optimization of exopolysaccharide extracted from a newly isolated halotolerant cyanobacterium, Acaryochloris Al-Azhar MNE ON864448.1 with antiviral activity

The structure, characterization and dual-activity of exopolysaccharide produced by Bacillus enclensis AP-4 from deep-sea sediments

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