Isolation and structural properties of aerial mycelium differentiation-inhibitory substances against Streptomyces scabiei causing potato common scab

Abstract

The inhibitor of aerial mycelium differentiation in Streptomyces scabiei, a pathogen of potato common scab, was searched. An actinomycete isolated from the field was found to produce inhibitors and two active substances were isolated as single peaks with HPLC. The molecular weights of inhibitors A and B were 1156.5 and 1140.5, respectively. Numbers of functional groups in inhibitor A were estimated by derivatization reactions. Inhibitor A seems to be a non-peptidic or non-saccharic substance because enzymatic treatments with pepsin, trypsin or glycosidases did not affect the LC-MS profiles or inhibitory activity.

Structural Determination of Hypnosin, a Spore Germination Inhibitor of Phytopathogenic Streptomyces sp. Causing Root Tumor in Melon (Cucumis sp.)

“…A ring nitrogen or nitrogenous group adjacent to the carboxyl group may have some contribution to activity. Dipicolinic acid (20) showed relatively strong activity, but its regioisomers, quinolinic acid (21) and cinchomeronic acid (22), had 1 / 10 and 1 / 25 the activity of 20.…”

“…In Streptomyces spp., inducers of differentiation of aerial mycelia from substrate mycelia and subsequent sporulation and their mode of action are well-studied ,, . Inhibitors of aerial mycelial differentiation have also been reported , . Hypnosin is the second germination inhibitor observed in Streptomyces spp.…”