Isolation and Structure Elucidation of a New Diterpene Glycoside from Stevia rebaudiana Bertoni

Abstract

A new minor diterpene glycoside has been isolated from a commercial extract of the leaves of Stevia rebaudiana and its structure was identified as 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-[2-O-(2-O-β-D-glucopyranosyl)-β-D-glucopyranosyl-3-O-b-D-glucopyranosyl-β-D-glucopyranosyl] ester (1) based on extensive NMR (1D and 2D) and mass spectroscopic data, and hydrolysis studies.

Employment of In Vitro Technique to Propagate Stevia (Stevia rebaudiana) Plant

“…Furthermore, stevia is famous with its medicinal value for example; it is an important source for the treatment of diabetes, as its intake does not negatively affect diabetics and without having a negative effect on the concentration of sugar in the blood, so it have potential application in human diet as sucrose substitute [3][4][5]. Therefore, the stevia plant is known as the sweet leaf plant, as it is unique in its concentrated sugar content whereas represents a raw material for the production of sugar stevioside which is known as a super-sweet glycosides [6,7]. Although this compound has low calories, it is 300 times sweeter than food sugar [8,9].…”

Direct Shoot Bud Proliferation Protocol from Stevia rebaudiana Leaf Culture for Healthy Biomass Production

“…Candyleaf Stevia rebaudiana Bertoni (Asteraceae family), a medicinal and valuable plant containing sweetener compounds in their leaves, that are found to be 100-300 times sweeter than sucrose in its leaves (Ahmad et al, 2011). Stevia has been used as a natural sweetener in a broad range of products in the food and soft drink industry for decades since its leaves contain stevioside and rebaudioside (Chaturvedula & Meneni, 2018). These compounds are being used for the treatment of diabetes because they are non-toxic, lowcalorie, and non-mutagenic (Aman et al, 2013).…”