Isolation and structure of the pectin lyase D-encoding gene from Aspergillus niger

Nucleotide Sequence of the Pectate Lyase Gene from Alkali-tolerantBacillussp. YA-14

“…2 ) Several genes of these pectolytic enzymes have been cloned and sequenced from Erwinia carotovora,3 -5) Erwinia chrysanthemi, 6 -8) and Aspergillus niger. 9) But there has been relatively few reports on the members of Bacillaceae family producing pectate lyase. Previously we reported the cloning of the pe IK gene from alkali·tolerent Bacillus sp.…”

The Refined Three-Dimensional Structure of Pectate Lyase E from Erwinia chrysanthemi at 2.2 A Resolution

“…Moreover, a distantly related pectate lyase from Bacillus subtilis has also been shown to have a similar fold (Pickersgill et al, 1994), suggesting that a11 proteins with certain sequence signatures are likely to have a similar structure. This group includes a11 extracellular pectate lyases, with the exception of Fusariunr solani pectate lyase (GonzalesCandelas and Kolattukudy, 1992), as well as funga1 pectin lyases (Gysler et al, 1990;Kuster-Van Someren et al, 1992), and pollen and style plant proteins (Wing et al, 1989;Budelier et al, 1990;Rafner et al, 1991;Rogers et al, 1992). The structural similarities may or may not extend to functional similarities, depending on the sequence similarities in the active site regions.…”

Molecular cloning and nucleotide sequence of the gene encoding phosphate‐inducible pectin lyase of Bacillus subtilis

“…So far, seven PNL genes (three from Aspergillus niger [19][20][21], two from E. carotovora [22,23], one from Glomerella cingulata [24] and one from P. marginalis [25]) have been cloned and sequenced. Comparison of the amino acid sequence of PPase-R with those of the above seven PNLs revealed low homologies with the four eukaryotic genes (Aspergillus and Glomerella) but higher homologies with the three other prokaryotic genes ( Fig.…”