Isolation and study of a ubiquitously expressed tomato pectin methylesterase regulatory region

Tiznado-Hernández, Martín-Ernesto; Gaffé, Joël; Handa, Avtar K.

doi:10.2225/vol7-issue1-fulltext-1

Cited by 2 publications

(2 citation statements)

References 42 publications

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“…The pH was constantly adjusted to 7.5 with 0.025 mol L −1 NaOH and the pH changes causing the reaction to equilibrate at 7.0 were recorded for 10 min. A unit of PME activity was defined as the mmoles of H + produced per minute per milligram of protein 17…”

Section: Methodsmentioning

confidence: 99%

“…A unit of PME activity was defined as the mmoles of H + produced per minute per milligram of protein. 17 β-Galactosidase was extracted from 20 g of fresh-cut papaya tissue and was in accordance with a previously published technique. 16 The protein content of each extract was determined by the Bradford method.…”

Section: Extraction and Assay Of Texture-related Enzymesmentioning

confidence: 99%

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Effect of chitosan coating in preventing deterioration and preserving the quality of fresh‐cut papaya ‘Maradol’

et al. 2008

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BACKGROUND: Chitosan can form antimicrobial, semi-permeable barriers that limit gas exchange and reduces water loss in fruits. Consumer interest in fresh-cut papaya fruit is leading to increasing demand because of its sensorial and antioxidant properties. However, papaya is a highly perishable product that is prone to loss of weight, loss of firmness and microbial attack. The aim of this study was to evaluate the effect of chitosan coatings on the overall quality of fresh-cut papaya. Chitosan coatings of low (LMWC), medium (MMWC) and high (HMWC) molecular weights, at concentrations of 0.01 and 0.02 g mL −1 , were applied to fresh-cut papaya cubes. The treated cubes were stored at 5• C and changes in quality were evaluated.

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Section: Methodsmentioning

confidence: 99%

Section: Extraction and Assay Of Texture-related Enzymesmentioning

confidence: 99%

Effect of chitosan coating in preventing deterioration and preserving the quality of fresh‐cut papaya ‘Maradol’

et al. 2008

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Simultaneous growth and emission measurements demonstrate an interactive control of methanol release by leaf expansion and stomata

Hüve

Christ

Kleist

et al. 2007

Journal of Experimental Botany

189

198

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Emission from plants is a major source of atmospheric methanol. Growing tissues contribute most to plant-generated methanol in the atmosphere, but there is still controversy over biological and physico-chemical controls of methanol emission. Methanol as a water-soluble compound is thought to be strongly controlled by gas-phase diffusion (stomatal conductance), but growth rate can follow a different diurnal rhythm from that of stomatal conductance, and the extent to which the emission control is shared between diffusion and growth is unclear. Growth and methanol emissions from Gossypium hirsutum, Populus deltoides, and Fagus sylvatica were measured simultaneously. Methanol emission from growing leaves was several-fold higher than that from adult leaves. A pronounced diurnal rhythm of methanol emission was observed; however, this diurnal rhythm was not predominantly determined by the diurnal rhythm of leaf growth. Large methanol emission peaks in the morning when the stomata opened were observed in all species and were explained by release of methanol that had accumulated in the intercellular air space and leaf liquid pool at night in leaves with closed stomata. Cumulative daily methanol emissions were strongly correlated with the total daily leaf growth, but the diurnal rhythm of methanol emission was modified by growth rate and stomatal conductance in a complex manner. While in G. hirsutum and in F. sylvatica maxima in methanol emission and growth coincided, maximum growth rates of P. deltoides were observed at night, while maximum methanol emissions occurred in the morning. This interspecific variation was explained by differences in the share of emission control by growth processes, by stomatal conductance, and methanol solubilization in tissue water.

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Isolation and study of a ubiquitously expressed tomato pectin methylesterase regulatory region

Cited by 2 publications

References 42 publications

Effect of chitosan coating in preventing deterioration and preserving the quality of fresh‐cut papaya ‘Maradol’

Effect of chitosan coating in preventing deterioration and preserving the quality of fresh‐cut papaya ‘Maradol’

Simultaneous growth and emission measurements demonstrate an interactive control of methanol release by leaf expansion and stomata

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