Isolation and13C-NMR Studies on Cathovaline, an Alkaloid from the Leaves ofCatharanthus roseus

Ali, İrshad; Chaudhry, M. Q.

doi:10.1055/s-2007-969546

Planta Med

1985

DOI: 10.1055/s-2007-969546

|View full text |Cite

Isolation and¹³C-NMR Studies on Cathovaline, an Alkaloid from the Leaves ofCatharanthus roseus

İrshad Ali¹,

M. Q. Chaudhry²

Abstract: Studies on the alkaloidal constituents of the leaves of CATHARANTHUS ROSEUS have resulted in the isolation of cathovaline. (13)C-NMR studies have been carried out on the alkaloid using ADEPT and APT pulse sequences. Cathovaline has not been previously reported from this plant but has been isolated from the roots of CATHARANTHUS LANCEUS (Boj. ex. A. De) pich (1) and CATHARANTHUS OVALIS Markgraf, sp. nov. (2).

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2010

2021

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Tonoplast and Peroxisome Targeting of γ-tocopherolN-methyltransferase Homologs Involved in the Synthesis of Monoterpene Indole Alkaloids

Κουδούνας

Guirimand²,

Rojas

et al. 2021

Plant and Cell Physiology

View full text Add to dashboard Cite

Many plant species from the Apocynaceae, Loganiaceae and Rubiaceae families evolved a specialized metabolism leading to the synthesis of a broad palette of monoterpene indole alkaloids (MIAs). These compounds are believed to constitute a cornerstone of the plant chemical arsenal but above all several MIAs display pharmacological properties that have been exploited for decades by humans to treat various diseases. It is established that MIAs are produced in planta due to complex biosynthetic pathways engaging a multitude of specialized enzymes but also a complex tissue and subcellular organization. In this context, N-methyltransferases (NMTs) represent an important family of enzymes indispensable for MIA biosynthesis but their characterization has always remained challenging. In particular, little is known about the subcellular localization of NMTs in MIA-producing plants. Here, we performed an extensive analysis on the subcellular localization of NMTs from four distinct medicinal plants but also experimentally validated that two putative NMTs from Catharanthus roseus exhibit N-methyltransferase activity. Apart from providing unprecedented data regarding the targeting of these enzymes in planta, our results point out an additional layer of complexity to the subcellular organization of the MIA biosynthetic pathway by introducing tonoplast and peroxisome as new actors of the final steps of MIA biosynthesis.

show abstract

Tonoplast and Peroxisome Targeting of γ-tocopherolN-methyltransferase Homologs Involved in the Synthesis of Monoterpene Indole Alkaloids

Κουδούνας

Guirimand²,

Rojas

et al. 2021

Plant and Cell Physiology

View full text Add to dashboard Cite

show abstract

Isolation and characterization of antineoplastic alkaloids from Catharanthus roseus l. Don. cultivated in Egypt

Shams¹,

Nazif²,

Azim³

et al. 2010

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus roseus L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid chromatographic column on silica gel : aluminium oxide (1:1) mixed bed vacuum liquid chromatography (VLC), Charcoal column, and finally purified by centrifugally accelerated radial chromatography (Chromatotrone).

show abstract

Real time sequence characterized amplified region (RT-SCAR) marker: Development and its application for authentication and quantification of Catharanthus roseus L. Don.

Chaudhary¹

2012

J. Med. Plants Res.

View full text Add to dashboard Cite

The objective of the present study was to develop a Real Time-Sequence Characterized Amplified Region (RT-SCAR) of Catharanthus roseus, an effective molecular marker for authentication and quantification. Here, we made a comparative study of SCAR and RT-SCAR marker. These newer RT-SCAR methods are useful for the correct identification and quantification of C. roseus. In particular, this technique is very attractive and can be used as a novel tool for quantification of C. roseus when the SCAR results cannot be quantified and the RAPD results are not consistent. RAPD analysis of collected samples from different geographical locations in India was carried out with 25 random primers. The RAPD polymorphic band was cloned, sequenced and from the sequence information, primers pair for SCAR was developed. The same primers was used for RT-SCAR marker by using SYBR green fluorescence-based detection in Real Time PCR. Utilizing this newly specific RT-SCAR marker, we detected the presence and quantity of C. roseus in all the analyzed several samples, by aiding 10% its substitute and adulterants at time of DNA isolation. No difference was found in pure sample and 10% adulterant sample with the help of SCAR but in the case of RT-SCAR difference was found in the DNA sample. This analytical strategy could be used further for large scale quantification of C. roseus's market samples.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Isolation and¹³C-NMR Studies on Cathovaline, an Alkaloid from the Leaves ofCatharanthus roseus

Cited by 3 publications

References 7 publications

Tonoplast and Peroxisome Targeting of γ-tocopherolN-methyltransferase Homologs Involved in the Synthesis of Monoterpene Indole Alkaloids

Tonoplast and Peroxisome Targeting of γ-tocopherolN-methyltransferase Homologs Involved in the Synthesis of Monoterpene Indole Alkaloids

Isolation and characterization of antineoplastic alkaloids from <i>Catharanthus roseus</i> l. Don. cultivated in Egypt

Real time sequence characterized amplified region (RT-SCAR) marker: Development and its application for authentication and quantification of Catharanthus roseus L. Don.

Contact Info

Product

Resources

About