Isolation and temporal endospermal expression of γ-kafirin gene of grain sorghum (Sorghum bicolor L. moench) var. M 35-1 for introgression analysis of transgene

Genetic Basis of Protein Digestibility in Grain Sorghum

“…The predicted amino acid sequences of the two genes have a low sequence identity of 28%, but the 50-kDa g-kafirin shares 86% amino acid sequence identity with the 50-kDa sugarcane (Saccharum officinarum L.) g-canein (Laidlaw et al, 2010). With maize and pearl millet (Pennisetum glaucum L.) g-prolamins, sorghum g-kafirin shares a nucleotide sequence identity of 85% and amino acid sequence identity of ?78% (Bansal et al, 2008). With maize and pearl millet (Pennisetum glaucum L.) g-prolamins, sorghum g-kafirin shares a nucleotide sequence identity of 85% and amino acid sequence identity of ?78% (Bansal et al, 2008).…”

“…None of the g-kafirin variants were found to affect protein digestibility over the other (Cremer et al, 2014a). g-Kafirin gene is expressed in developing sorghum seeds starting at 7 d after pollination, with transcript accumulation reaching maximum at 21 d after pollination (Bansal et al, 2008). g-Kafirin gene is expressed in developing sorghum seeds starting at 7 d after pollination, with transcript accumulation reaching maximum at 21 d after pollination (Bansal et al, 2008).…”

“…An additional g-kafirin variant was recently identified, which has been shown to be associated with increased lysine content in Mexican food-grade white sorghum cultivars without a noticeable effect on grain protein content and protein digestibility (Chiquito-Almanza et al, 2016). g-Kafirin gene is expressed in developing sorghum seeds starting at 7 d after pollination, with transcript accumulation reaching maximum at 21 d after pollination (Bansal et al, 2008). This expression pattern is consistent with that of the corresponding protein in maize, g-zein, which accumulates and initiates protein body formation early during seed development (Holding, 2014).…”

“…This agrees with the detection of the high-molecular-weight g-kafirin protein homologous to other grass g-prolamins in protein extracts of mature sorghum grain (Cremer et al, 2014a). With maize and pearl millet (Pennisetum glaucum L.) g-prolamins, sorghum g-kafirin shares a nucleotide sequence identity of 85% and amino acid sequence identity of ?78% (Bansal et al, 2008). Laidlaw et al (2010) reported that the 27-kDa g-kafirin locus has five alleles in the S. bicolor germplasm, the majority of which are silent mutations without any bearing on amino acid sequence, leaving only two g-kafirin protein variants in cultivated sorghum and one unique allele in sorghum wild relative Sorghum propinquum (Kunth) Hitchc.…”

Ileal amino acids digestibility of sorghum in weaned piglets and growing pigs

“…Sorghum (S) ranks fifth among the cereals produced worldwide (Bansal et al, 2008). A characteristic of S is its huge variability in chemical composition and consequently in its nutritive value (Mossé et al, 1988).…”