Isolation and toxicity of Bacillus thuringiensis from potato-growing areas in Bolivia

Hernández, Carmen Sara; Andrew, René; Bel, Yolanda; Ferré, Juan

doi:10.1016/j.jip.2004.10.006

Cited by 41 publications

(28 citation statements)

References 26 publications

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“…Some of our new B. thuringiensis isolates from both soil and insects produced high mean percentage mortality in C. quinquefasciatus, A. gemmatalis or both, confirming the reports of various authors that both soil and insects are good sources for new entomopathogenic B. thuringiensis strains (Hansen et al, 1997;Schnepf et al, 1998;Kim, 2000;Cavados et al, 2001;Quesada-Moraga et al, 2004;Hajaij et al, 2005;Hernandez et al, 2005).…”

Section: Discussionsupporting

confidence: 88%

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Bacillus thuringiensis isolates entomopathogenic for Culex quinquefasciatus (Diptera: Culicidae) and Anticarsia gemmatalis (Lepidoptera: Noctuidae)

et al. 2010

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Samples of the Bacillus thuringiensis (Bt) were collected from soil and insects. Eight isolates were selected from rural soil, 15 from urban soil and 11 from insects. These were evaluated for entomopathogenicity against larvae of Anticarsia gemmatalis and Culex quinquefasciatus. The pathogenicity tests showed that a higher percentage of isolates were active against A. gemmatalis (60%) compared to C. quinquefasciatus (31%). Probit analysis (LC 50 ) indicated that against A. gemmatalis four of the isolates presented values similar to the reference strain against A. gemmatalis, while against C. quinquefasciatus one isolate showed an LC 50 similar to the reference strain (IPS-82). SDS-PAGE characterisation of two isolates showed a 27 kDa protein fraction related to the Bt subspecies israelensis cytolytic toxin (cyt) gene. One 130 kDa protein, possibly related to the Bt crystal inclusions (cry1) gene, was identified in the other two isolates, which were more toxic for lepidoptera; another isolate presented a protein of 100 kDa. Some new local Bt isolates had similar LC50 probit values to the reference strains.Keywords: Anticarsia gemmatalis, Bacillus thuringiensis, Cry proteins, Culex quinquefasciatus, isolation. Isolados de

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Section: Discussionsupporting

confidence: 88%

“…The 36% of B. thuringiensis recovered from insects was similar to the 40% reported by , but less than the 60% obtained by Hernandez et al (2005).…”

Section: Discussionsupporting

confidence: 74%

Bacillus thuringiensis isolates entomopathogenic for Culex quinquefasciatus (Diptera: Culicidae) and Anticarsia gemmatalis (Lepidoptera: Noctuidae)

et al. 2010

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“…The results showed that all 5 isolates exhibited strong toxicity to C. pipiens and C. maculatus while, 2 isolates (YD10 and YH18) had larvicidal against S. littoralis. These results confirmed the results of microscopic examination of isolates no YD10 and YH18 which showed bipyramidal parasporal inclusions and exhibited strong toxicity to Lepidoptera [22,32,40,45].…”

Section: Discussionsupporting

confidence: 86%

Occurrence of Bacillus thuringiensis and their phages in Yemen soil

El–Didamony

2013

VirusDis.

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Bacillus thuringiensis (Bt) isolates were found in all samples of soil in nine Governorates of Yemen. From 384 isolates of Bacillus recovered from these soil samples after acetate selection, 104 isolates (27.1 %) were Bt. Five isolates of Bt were selected and insecticidal activity was tested against Culex pipiens, Callosobruchus maculatus and Spodoptera littoralis. The Bt isolate YH18 gave toxicity to all tested insects larvae. This study extended to isolate phages active against the selected Bt isolates. Five phages were isolated and classified into two groups of tailed phages. Four phages with long non-contractile tails and hexagonal heads (Siphoviridae) and one phage with very short tail and isometric head (Podoviridae). Susceptibility of selected Bt to infect by these phages was studied by spot-test technique. Also the Bt isolate no YH18 was resistant to all tested phages. This is the first report illustrates the diversity and the abundance of Bt and Bt phage in Yemen soil.

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“…Colonies were then classified into different groups based on their crystal shape (LopezPazos et al, 2007). Potential Bt candidates were sub-cultured and purified on nutrient agar plates and then stored as stock culture in sterile liquid Nutrient Broth containing 50% glycerol at −20°C (Hernandez et al, 2005).…”

Section: Phase Contrast Microscopymentioning

confidence: 99%

Isolation and Characterization of Native Isolates of Bacillus thuringiensis from Vidarbha Region

Padole¹,

Moharil²,

Ingle³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Isolation and toxicity of Bacillus thuringiensis from potato-growing areas in Bolivia

Cited by 41 publications

References 26 publications

Bacillus thuringiensis isolates entomopathogenic for Culex quinquefasciatus (Diptera: Culicidae) and Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Bacillus thuringiensis isolates entomopathogenic for Culex quinquefasciatus (Diptera: Culicidae) and Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Occurrence of Bacillus thuringiensis and their phages in Yemen soil

Isolation and Characterization of Native Isolates of Bacillus thuringiensis from Vidarbha Region

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