2012
DOI: 10.1007/s00253-012-4578-y
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Isolation, biochemical characterization, and cloning of a bacteriocin from the poultry-associated Staphylococcus aureus strain CH-91

Abstract: Staphylococcus aureus strain CH-91, isolated from a broiler chicken with atopic dermatitis, has a highly proteolytic phenotype that is correlated with the disease. We describe the isolation and biochemical and molecular characterization of the AI-type lantibiotic BacCH91 from S. aureus CH-91 culture medium. The bacteriocin was purified using a three-stage procedure comprising precipitation with ammonium sulfate, extraction with organic solvents, and reversed-phase HPLC. The BacCH91 peptide is thermostable and … Show more

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Cited by 35 publications
(22 citation statements)
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“…Nevertheless, it is worth noting that a variety of pathogenic bacteria (e.g., Staphylococcus aureus) also produce bacteriocin effective against competing bacteria. 121 Horizontal gene transfer Horizontal gene transfer is "the non-genealogical transmission of genetic material from one organism to another." 122 It is mediated by processes such as conjugation, transformation, and transduction and is an effective mechanism which contributes to bacterial diversification and facilitates bacterial adaptation to new environments.…”
Section: Competition For Nutrient and Attachment Sitementioning
confidence: 99%
“…Nevertheless, it is worth noting that a variety of pathogenic bacteria (e.g., Staphylococcus aureus) also produce bacteriocin effective against competing bacteria. 121 Horizontal gene transfer Horizontal gene transfer is "the non-genealogical transmission of genetic material from one organism to another." 122 It is mediated by processes such as conjugation, transformation, and transduction and is an effective mechanism which contributes to bacterial diversification and facilitates bacterial adaptation to new environments.…”
Section: Competition For Nutrient and Attachment Sitementioning
confidence: 99%
“…Hominicin from S. hominis displayed potent activity against multiple strains including S. aureus ATCC 25923, MRSA ATCC 11435, and vancomycin-intermediate S. aureus CCARM 3501[97,98], at MICs of 0.06µg/mL, 0.96 µg/mL, and 3.82 µg/mL, respectively[98]. BacCh91, produced by S. aureus CH91, inhibited four test strains of S. aureus (ATCC25293, Newman, M-122 and RN4220), with an MIC of 4.0-6.0 µM[92]. Gallidermin, isolated from poultry-associated Staphylococcus gallinarum, has been shown to be bactericidal against both MRSA and methicillin-sensitive S. aureus (MSSA)[96,121].…”
mentioning
confidence: 99%
“…In addition, these hemolysins were fully soluble in 70% aqueous acetone, a volatile organic solvent which can be easily removed (Różalska, data not shown). This method was also successfully used in studies on bacteriocin produced by the strain of S. aureus CH-91 (Wladyka et al, 2013).…”
Section: Resultsmentioning
confidence: 99%