1979
DOI: 10.1099/00221287-111-2-387
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Isolation by Differential and Zonal Centrifugation of Minicells Segregated by Escherichia coli

Abstract: 387Minicells segregated from Escherichia coli ~9 2 5 carrying a drug-resistance plasmid were separated from nucleated cells by differential centrifugation and purified by rate-zonal centrifugation in sucrose gradients. Minicells purified in this way were capable of donating the plasmid to nucleated cells. They also incorporated thymidine, uridine and methionine into macromolecules. Methods are described for purification of plasmid-containing minicells on a scale large enough to allow isolation of DNA, DNA poly… Show more

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Cited by 3 publications
(1 citation statement)
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“…For instance, the combination of antibiotics treatment with filtration or centrifugation can kill bacteria but not harm non-dividing minicells, thus achieving higher yield. [55,56] Park et al utilized the lytic proteins leading to the autolysis of parent bacteria, to have a high purity of minicells after density gradient centrifugation. [58] Most novel purification methods rely on the different responses of minicells and parent bacteria toward treatment.…”
Section: Bacterial Minicellsmentioning
confidence: 99%
“…For instance, the combination of antibiotics treatment with filtration or centrifugation can kill bacteria but not harm non-dividing minicells, thus achieving higher yield. [55,56] Park et al utilized the lytic proteins leading to the autolysis of parent bacteria, to have a high purity of minicells after density gradient centrifugation. [58] Most novel purification methods rely on the different responses of minicells and parent bacteria toward treatment.…”
Section: Bacterial Minicellsmentioning
confidence: 99%