Isolation, cell culture and immunocytochemical characterization of oviduct epithelial cells of the cow

Joshi, M. S.

doi:10.1530/jrf.0.0830249

Cited by 52 publications

(37 citation statements)

References 23 publications

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“…We have described a method to isolate a large number of bovine oviduct epithelial cells uncontaminated by stromal cells using collagenase, and to prepare cell cultures with cytoarchitecture closely resembling those of the intact oviduct epithelial cells. The cultured epithelial cells contained cytokeratin and also elaborated the secretory proteins as demonstrated by immunocytology and immunoblot analyses (Joshi, 1988). It is interesting to note that the culture ciliated epithelial cells which attached to the plastic maintained their cilia only for 4-5 days while unattached ciliated cells continued their ciliary activity in the culture medium for a longer time.…”

Section: Discussionmentioning

confidence: 87%

“…The monolayer of bovine oviduct epithelial cells on plain plastic demonstrated inadequate differentiation with very few secretory vesicles. The secretory vesicles failed to migrate to apical plasmalemma (Joshi, 1988).…”

Section: Discussionmentioning

confidence: 97%

“…The influence of oviduct secretions on capacitation of spermatozoa (McNutt and Killian, 1991) on development of early embryo (Archibong et al, 1989;Bavister, 1988;Bongso et al, 1989a;Ellington et al, 1990;Eyestone et al, 1987;Gandolfi and Moor, 1987;Goldberg et al, 1991;White et al, 1989) has been well documented. Oviduct secretory cells have been shown to synthesize and secrete specific proteins in vivo and in vitro (Boice et al, 1990a;Buhi et al, 1989;Joshi, 1988; and that the synthesis and release is influenced by steroid hormones (Erickson-Lawrence et al, 1989;Joshi, 1991;Malayer et al, 1988; and during co-culture with spermatozoa (Ellington et al, 1993). Transfer of the oviductal antigen to oocyte (Boice et al, 1990b;Gandolfi et al, 1991;Kan et al, 1989;Kapur and Johnson, 1985;Leveille et al, 1987;Oikawa et al, 1988) and to spermatozoa (Lippes and Wagh, 1989) has been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

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Isolation, cell culture, and characterization of oviduct epithelial cells of the cow

Joshi

1995

Microscopy Res & Technique

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This report describes an easy method of isolation and cell culture of the epithelial cells of cow oviduct. Incubation of cow oviduct with 0.1 mg/ml collagenase in the lumen for 90 minutes helped to dislodge large numbers of ciliated and secretory epithelial cells. The isolated cells, when seeded on plastic, proliferated very quickly and became confluent in 8-10 days in 35 mm Petri dishes. The isolated ciliated cells which attached to the plastic dish lost their cilia after 4-5 days in culture. The cultured epithelial cells were keretin positive. The isolated bovine oviduct epithelial cells, when cultured on plastic precoated with 10 mg/ml matrigel, organized themselves into hollow tubes or spheres with microvilli directed towards the lumen. The epithelial cells seeded on 2 mg/ml matrigel became subconfluent in 15-20 days after seeding. The histoarchitecture of the secretory cells growing in vitro on matrigel resembled that of intact oviduct secretory epithelial cells. Occasional ciliated cells containing large number of mitochondria were observed in the monolayer cultured on 2 mg/ml matrigel substratum but possessed few cilia. The oviduct epithelial cells cultured on 2 mg/ml matrigel incorporated 35S-methionine linearly into protein up to 8 hours in the presence of estradiol or progesterone. The fluorograph of the newly synthesized proteins indicated the presence of an additional 60 kd protein in the cell extract of epithelial cells incubated with estradiol.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Isolation, cell culture, and characterization of oviduct epithelial cells of the cow

Joshi

1995

Microscopy Res & Technique

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“…In in vitro primary culture conditions, OECs display epithelial cell morphology -appearing as densely packed polygonal cells. In the first days of primary culture, ciliated cells are present, disappearing in secondary culture [17]- [19]. Secretory cells are located along the entire length of the fallopian tube in vivo.…”

Section: Discussionmentioning

confidence: 99%

Amino acids metabolism and degradation is regulated during porcine oviductal epithelial cells (OECs) primary culture in vitro – a signaling pathways activation approach

Kranc

Jankowski

Budna

et al. 2018

Medical Journal of Cell Biology

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The ovary is part of the reproductive system, possessing very important functions in the reproduction process (ovum and embryo transfer, providing a suitable environment for sperm capacitation, etc.). There are two types of cells in the fallopian tubes: alveolar and secretive cells. These study shows the metabolic processes in pig oviductal epithelial cells associated with the activation of signaling pathways of amino acids metabolism and degradation during long-term in vitro culture. Oviductal epithelial cells from 45 colonies in the anestrous phase of the estrous cycle have been utilized in this study. RNA extract from the OEC primary cultures was pooled after 24h, 7days, 15 days and 30 days from the beginning of culture and the transcriptome investigated by Affymetrix® Porcine Gene 1.1 ST. From the whole transcript that consisted of 2009 different genes, 1537 were upregulated and 995 were downregulated after 7 days of culture, 1471 were upregulated and 1061 were downregulated after 15 days of culture and 1329 were upregulated and 1203 were downregulated after 30 days of culture. The results of these studies provide, for the first time, information on the activation of metabolic pathways of amino acids such as valine, leucine, isoleucine, cysteine, and methionine in the investigated tissue. They also indicate genes that may be OECs-specific genetic markers that are expressed or upregulated during long-term in vitro culture.Running title: regulation of amino acid signaling pathways

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“…Oviductins have been identified and characterized in many rodents like hamsters (Leveille et al, 1987;Oikawa et al, 1988;Robitaille et al, 19881, mice (Kapur andJohnson, 1985, 1986), and rabbits (Oli-0 1995 WILEY-LISS. INC phant and Ross, 1982;Oliphant et al, 1984), as well as in several mammalian species such as sheep (Gandolfi et al, 1989), baboons (Fazleabas and Verhage, 1986;Verhage et al, 19901, pigs (Buhi et al, 1989, 19901, cows (Joshi, 1988;Ma-layer et al, 1988), and humans (Wagh and Lippes, 1989). Several immunocytochemical studies also demonstrated the association of oviductins with the zona pellucida of postovulatory oocytes in the hamster (Araki et al, 1987;Kan et al, 1988Kan et al, , 1989Abe and Oikawa, 1990) and the mouse (Kapur and Johnson, 1985,1986.…”

mentioning

confidence: 97%

Stage‐specific immunolabeling for oviductin in the secretory granules of the oviductal epithelium of the golden hamster during the estrous cycle

Roux

Kan

1995

Anat. Rec.

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Together, these quantitative results indicate that expression of oviductin in the secretory granules of the hamster oviductal secretory cells is stage specific. Maximum labeling for the antigen coincides with the time of ovulation suggesting an important role for the oviductal epithelium in contributing its secretory product to the zona pellucida of oocytes freshly released from the ovary. Since the oviduct is the site of sperm-egg interaction and where fertilization and early embryo development take place, the maximal production of oviductin at the time of ovulation may facilitate some of these crucial steps during the intricate process of reproduction.

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Isolation, cell culture and immunocytochemical characterization of oviduct epithelial cells of the cow

Cited by 52 publications

References 23 publications

Isolation, cell culture, and characterization of oviduct epithelial cells of the cow

Isolation, cell culture, and characterization of oviduct epithelial cells of the cow

Amino acids metabolism and degradation is regulated during porcine oviductal epithelial cells (OECs) primary culture in vitro – a signaling pathways activation approach

Stage‐specific immunolabeling for oviductin in the secretory granules of the oviductal epithelium of the golden hamster during the estrous cycle

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