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In this study, the antimicrobial activity of some medicinal plant seeds from the Apiaceae family which are anise, cumin, and fennel was investigated. Moreover, ethanol (70%) and methanol (80%) extracts of anise, cumin, and fennel seeds and essential oils of them were performed for this aim. Escherichia coli ATCC 25922 and Salmonella Enteritidis ATCC 13076, Bacillus subtilis ATCC 6633, Streptecoccus mutans ATCC 25175 and Listeria monocytogenes ATCC7644 were utilized as test microorganisms. Then, essential oils and ethanol extracts of cumin seeds showed the highest antimicrobial effects on almost all of the test microorganisms. While methanol extracts of fennel seeds had the highest antimicrobial effects against St. mutans and B. subtilis when compared to other methanol extracts. Additionally, methanol extracts of anise seeds indicated the highest effect on E. coli. However, all types of medicinal plant seeds had antimicrobial effects on test microorganisms in various severities (7.00-20.00 mm zone diameter) except L. monocytogenes. Thus, the results support existing scientific evidence for the evaluation of these plants as natural antimicrobial agents in several industries including food and drugs.
In this study, the antimicrobial activity of some medicinal plant seeds from the Apiaceae family which are anise, cumin, and fennel was investigated. Moreover, ethanol (70%) and methanol (80%) extracts of anise, cumin, and fennel seeds and essential oils of them were performed for this aim. Escherichia coli ATCC 25922 and Salmonella Enteritidis ATCC 13076, Bacillus subtilis ATCC 6633, Streptecoccus mutans ATCC 25175 and Listeria monocytogenes ATCC7644 were utilized as test microorganisms. Then, essential oils and ethanol extracts of cumin seeds showed the highest antimicrobial effects on almost all of the test microorganisms. While methanol extracts of fennel seeds had the highest antimicrobial effects against St. mutans and B. subtilis when compared to other methanol extracts. Additionally, methanol extracts of anise seeds indicated the highest effect on E. coli. However, all types of medicinal plant seeds had antimicrobial effects on test microorganisms in various severities (7.00-20.00 mm zone diameter) except L. monocytogenes. Thus, the results support existing scientific evidence for the evaluation of these plants as natural antimicrobial agents in several industries including food and drugs.
Garden thyme (Thymus vulgaris L.) is an annual herbaceous plant of the family Lamiaceae. It can be found both in the wild and as a cultivated plant in Bulgaria. This study is aimed at assessing the garden thyme essential oil composition and antioxidant activities, which were collected from three different areas in Bulgaria. The essential oils were obtained by hydrodistillation and analyzed by gas chromatography–mass spectrometry (GC-MS). The main compounds in the garden thyme essential oils (over 2%) were as follows: thymol (42.88–53.55%), p-cymene (14.25–25.51%), γ-terpinene (6.58–15.51%), borneol (2.75–3.57%), carvacrol (2.00–3.02%), β-linalool (2.07–2.31%), cis-sabinene hydrate (4.05%), eucalyptol (1.08–3.65%), α-terpinene (1.01–3.24%), carvacrol methyl ether (1.18–3.02%), and thymol methyl ether (2.26–3.16%). The oils were mainly composed of oxygenated phenyl propanoids, and all the essential oils belonged to the chemotype thymol. Antioxidant activities were measured by DPPH (2,2-diphenyl-1-picrylhydrazyl radical) and ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] assays. All the samples exhibited antioxidant activity relative to the DPPH radical (from 143.20 mM TE/mL to 165.91 mM TE/mL) and by the ABTS method (from 121.67 mM TE/mL to 127.62 mM TE/mL). These garden thyme essential oils could be used as natural antioxidants for food and as nutraceuticals.
In this chapter, the authors present nanomaterials for biomedical application as promising technology of drug delivery, biosensors, and tissue engineering. Nanomaterials have revolutionized the field of biomedical applications because they provide sophisticated solutions for tissue engineering, biosensing, and drug administration through treating and diagnosing a variety of human ailments, including cancer, heart disease, dental conditions, and disorders of the central and peripheral nervous systems. Nanomaterials play a crucial role in achieving sustainable development goals by offering innovative solutions across various health sectors. In this chapter, the author discussion attempts to indicate the revolutionary impact of nanomaterials on biomedical research and clinical practice through an extensive discussion of recent developments and problems in the areas of nanomaterials for biomedical applications: drug delivery, biosensors, and tissue engineering.
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