Isolation, characterization, and comparative genomic analysis of vB_PlaP_SV21, new bacteriophage of Paenibacillus larvae

Bozdeveci, Arif; Akpınar, Rahşan; Karaoğlu, Şengül Alpay

doi:10.1016/j.virusres.2021.198571

Cited by 8 publications

(6 citation statements)

References 67 publications

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“…The comparison between the whole genomes of the newly identified and the previously described P. larvae phages ( Oliveira et al, 2013 ; Beims et al, 2015 , 2020 ; Carson et al, 2015 ; Tsourkas et al, 2015 ; Abraham et al, 2016 ; Merrill et al, 2018 ; Walker et al, 2018 ; Yost et al, 2018 ; Ribeiro et al, 2019 ) proposes changes in some of the existent clusters (Vegas and Harrison; Ribeiro et al, 2019 ) and introduces new ones (more 18 clusters and 10 singletons). Before this study, four clusters (Fern, Harrison, Vegas, Halcyone) and two singletons, (API480 and Lily) had been reported ( Ribeiro et al, 2019 ) and recently, Bozdeveci et al (2021) introduced a new phage similar to API480, proposing a new cluster. This new data brings new insights into the diversity of P. larvae phages and supports the importance of a permanent feed and update of the available phage genomic data.…”

Section: Discussionmentioning

confidence: 99%

“…Temperate phages can remain in a dormant state within their host without triggering the lytic cycle, while at the same time having a considerable impact on the host genome variability and evolution, modulating the host fitness and virulence ( Brussow et al, 2004 ; Harrison and Brockhurst, 2017 ). To our knowledge, there has been no attempts to explore the role of temperate phages in the ecology and evolution of P. larvae despite studies reporting a total of 51 P. larvae prophages in the last 8 years ( Oliveira et al, 2013 ; Beims et al, 2015 , 2020 ; Carson et al, 2015 ; Tsourkas et al, 2015 ; Abraham et al, 2016 ; Merrill et al, 2018 ; Walker et al, 2018 ; Yost et al, 2018 ; Ribeiro et al, 2019 ; Bozdeveci et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…Besides genomics, the present analysis also contributes to widening the taxonomic diversity of P. larvae phages. So far, most of the reported phages are siphoviruses ( Oliveira et al, 2013 ; Stamereilers et al, 2018 ; Beims et al, 2020 ), with the exception of two podoviruses ( Ribeiro et al, 2019 ; Bozdeveci et al, 2021 ). The present analysis suggests the inclusion of at least 15 myoviruses in the collection.…”

Section: Discussionmentioning

confidence: 99%

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Analysis of intact prophages in genomes of Paenibacillus larvae: An important pathogen for bees

et al. 2022

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Paenibacillus larvae is the etiological agent of American Foulbrood (AFB), a highly contagious and worldwide spread bacterial disease that affects honeybee brood. In this study, all complete P. larvae genomes available on the NCBI database were analyzed in order to detect presence of prophages using the PHASTER software. A total of 55 intact prophages were identified in 11 P. larvae genomes (5.0 ± 2.3 per genome) and were further investigated for the presence of genes encoding relevant traits related to P. larvae. A closer look at the prophage genomes revealed the presence of several putative genes such as metabolic and antimicrobial resistance genes, toxins or bacteriocins, potentially influencing host performance. Some of the coding DNA sequences (CDS) were present in all ERIC-genotypes, while others were only found in a specific genotype. While CDS encoding toxins and antitoxins such as HicB and MazE were found in prophages of all bacterial genotypes, others, from the same category, were provided by prophages particularly to ERIC I (enhancin-like toxin), ERIC II (antitoxin SocA) and ERIC V strains (subunit of Panton-Valentine leukocidin system (PVL) LukF-PV). This is the first in-depth analysis of P. larvae prophages. It provides better knowledge on their impact in the evolution of virulence and fitness of P. larvae, by discovering new features assigned by the viruses.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Analysis of intact prophages in genomes of Paenibacillus larvae: An important pathogen for bees

et al. 2022

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“…In the identification of the causative agent of AFB, molecular techniques are applied. Polymerase chain reaction (PCR), real-time polymerase chain reaction (real-time PCR), and pulsed-field gel electrophoresis (PFGE) are the most commonly used in laboratories [ 2 , 4 , 16 , 22 , 25 , 30 , 46 , 47 , 49 , 57 , 58 ]. Real-time PCR analysis of the 16S rDNA gene of P. larvae represents an alternative, rapid diagnostic tool.…”

Section: Differential Diagnostics Of the Diseasementioning

confidence: 99%

American Foulbrood—Old and Always New Challenge

Matović¹,

Žarković²,

Debeljak³

et al. 2023

Veterinary Sciences

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American foulbrood (AFB) is exclusively an infectious disease of honey bee larvae (Apis mellifera) and their subspecies that is spread easily and rapidly and is often present in apiaries. Due to the resistance and pathogenicity of the bacterial causative agent of the disease, which has considerable epizootiological and economic significance for beekeeping, AFB was classified as a highly dangerous, infectious animal disease by the World Organization for Animal Health (WOAH). Considering the severity of the infection, a frequent occurrence, rapid and easy spread, epizooty and enzooty are common. We tried to present an overview of the latest information related to AFB through several chapters. In addition to the latest data on the etiology of the causative agent, the most important elements of the clinical signs of the disease are also listed. Along with an overview of classic microbiological and the latest molecular methods of diagnosis, we also discuss AFB treatment from its differential diagnostic aspect. We hope that through demonstrating the mentioned preventive measures and measures of good beekeeping practice, the review will contribute to the preservation of the health of bees and therefore the overall biodiversity of the planet.

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“…[19] First, most phages can be divided into three tail phage families based on their unique morphology, namely Myoviridae (contractile tail), Podoviridae (short tail), and Siphoviridae (long and noncontractile tail). [21] Additionally, phages can be categorized into four groups according to the types of genetic material in the capsid core, i.e., single-stranded DNA (ssDNA) phages, Morphology Siphoviridae Phage vB_AbaS_TCUP2199 [23] Myoviridae Phage vB_PlaM_Pd22F [24] Podoviridae Phage HH109 [25] Lifecycle Lytic phage Phage T7 [26] Temperate phage Phage 𝜆 [27] Nucleic acid dsRNA Phage phiZ98 [28] ssRNA Phage MS2 [29] ssDNA Vibriophage vB_VpaM_PG19 [30] dsDNA Phage vB_SalP_TR2 [31] Host range Narrow-host-range phage Bacteriophage of Marinobacter D1S9 [32] Broad-host-range phage R9Y-phage [33] double-stranded DNA (dsDNA) phages, single-stranded RNA (ss-RNA) phages, and double-stranded RNA (dsRNA) phages. [22] Moreover, more and more categorizations of phages are discovered, and new genome-based families are found.…”

Section: Properties Of Phagesmentioning

confidence: 99%

Recent Progress in Phage‐Based Nanoplatforms for Tumor Therapy

Li,

Peng,

Feng

et al. 2023

Small

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Nanodrug delivery systems have demonstrated a great potential for tumor therapy with the development of nanotechnology. Nonetheless, traditional drug delivery systems are faced with issues such as complex synthetic procedures, low reproducibility, nonspecific distribution, impenetrability of biological barrier, systemic toxicity, etc. In recent years, phage‐based nanoplatforms have attracted increasing attention in tumor treatment for their regular structure, fantastic carrying property, high transduction efficiency and biosafety. Notably, therapeutic or targeting peptides can be expressed on the surface of the phages through phage display technology, enabling the phage vectors to possess multifunctions. As a result, the drug delivery efficiency on tumor will be vastly improved, thereby enhancing the therapeutic efficacy while reducing the side effects on normal tissues. Moreover, phages can overcome the hindrance of biofilm barrier to elicit antitumor effects, which exhibit great advantages compared with traditional synthetic drug delivery systems. Herein, this review not only summarizes the structure and biology of the phages, but also presents their potential as prominent nanoplatforms against tumor in different pathways to inspire the development of effective nanomedicine.

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Isolation, characterization, and comparative genomic analysis of vB_PlaP_SV21, new bacteriophage of Paenibacillus larvae

Cited by 8 publications

References 67 publications

Analysis of intact prophages in genomes of Paenibacillus larvae: An important pathogen for bees

Analysis of intact prophages in genomes of Paenibacillus larvae: An important pathogen for bees

American Foulbrood—Old and Always New Challenge

Recent Progress in Phage‐Based Nanoplatforms for Tumor Therapy

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